Molecular cloning and characterization of a mouse spermatogenesis-related ring finger gene znf230
Complete cDNA of mouse gene znf230 was cloned by rapid amplification of cDNA ends (RACE). This cDNA is 982 base pairs (bp) in length and encodes a 230 amino acids (aa) protein with a ring finger domain at its C-terminus. Ninety-one and 98% nucleotide (nt) and aa sequence identity are observed with i...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2003-06, Vol.306 (2), p.347-353 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Qiu, Weimin Zhang, Sizhong Xiao, Cuiying Xu, Wenmin Ma, Yongxin Liu, Yunqiang Wu, Qiaqing |
| description | Complete cDNA of mouse gene
znf230 was cloned by rapid amplification of cDNA ends (RACE). This cDNA is 982 base pairs (bp) in length and encodes a 230 amino acids (aa) protein with a ring finger domain at its C-terminus. Ninety-one and 98% nucleotide (nt) and aa sequence identity are observed with its human homolog. Revealed by Northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR), this cDNA is only detected in testicular tissue, whereas the longer transcripts of 2.4 and 4.4
kb are ubiquitously expressed. The expression of
znf230 in testis is developmentally regulated and first detected at day 6 postnatal (pn). It reaches adult level between day 14 and 21 pn during which round spermatids appear in seminiferous tubule. The protein of
znf230 exhibits DNA binding activity and its ring finger domain may function as an activator module in transcription. Therefore, it is postulated that znf230 may function as a testis specific transcription factor during mouse spermatogenesis. |
| doi_str_mv | 10.1016/S0006-291X(03)00970-7 |
| format | Article |
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znf230 was cloned by rapid amplification of cDNA ends (RACE). This cDNA is 982 base pairs (bp) in length and encodes a 230 amino acids (aa) protein with a ring finger domain at its C-terminus. Ninety-one and 98% nucleotide (nt) and aa sequence identity are observed with its human homolog. Revealed by Northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR), this cDNA is only detected in testicular tissue, whereas the longer transcripts of 2.4 and 4.4
kb are ubiquitously expressed. The expression of
znf230 in testis is developmentally regulated and first detected at day 6 postnatal (pn). It reaches adult level between day 14 and 21 pn during which round spermatids appear in seminiferous tubule. The protein of
znf230 exhibits DNA binding activity and its ring finger domain may function as an activator module in transcription. Therefore, it is postulated that znf230 may function as a testis specific transcription factor during mouse spermatogenesis.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(03)00970-7</identifier><identifier>PMID: 12804569</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Blotting, Northern ; Blotting, Southern ; Blotting, Western ; Cloning, Molecular ; DNA, Complementary - metabolism ; DNA-Binding Proteins - chemistry ; DNA-Binding Proteins - genetics ; Gene Expression Regulation ; Gene Expression Regulation, Developmental ; Humans ; Male ; Mice ; Molecular Sequence Data ; Open Reading Frames ; Protein Binding ; Protein Biosynthesis ; Protein Structure, Tertiary ; RACE ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Seminiferous Tubules - metabolism ; Sequence Homology, Amino Acid ; Southwestern blot ; Spermatogenesis ; Testis - metabolism ; Time Factors ; Tissue Distribution ; Transcription Factors ; Transcription, Genetic ; Transcriptional regulation ; Two-Hybrid System Techniques ; Yeast one-hybridization ; znf230 gene</subject><ispartof>Biochemical and biophysical research communications, 2003-06, Vol.306 (2), p.347-353</ispartof><rights>2003 Elsevier Science (USA)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-a992928ef51bcd3b0df7cd270ae1ff60ea081b4feeef759cf4e37f37a5c0c5e33</citedby><cites>FETCH-LOGICAL-c458t-a992928ef51bcd3b0df7cd270ae1ff60ea081b4feeef759cf4e37f37a5c0c5e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(03)00970-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12804569$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qiu, Weimin</creatorcontrib><creatorcontrib>Zhang, Sizhong</creatorcontrib><creatorcontrib>Xiao, Cuiying</creatorcontrib><creatorcontrib>Xu, Wenmin</creatorcontrib><creatorcontrib>Ma, Yongxin</creatorcontrib><creatorcontrib>Liu, Yunqiang</creatorcontrib><creatorcontrib>Wu, Qiaqing</creatorcontrib><title>Molecular cloning and characterization of a mouse spermatogenesis-related ring finger gene znf230</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Complete cDNA of mouse gene
znf230 was cloned by rapid amplification of cDNA ends (RACE). This cDNA is 982 base pairs (bp) in length and encodes a 230 amino acids (aa) protein with a ring finger domain at its C-terminus. Ninety-one and 98% nucleotide (nt) and aa sequence identity are observed with its human homolog. Revealed by Northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR), this cDNA is only detected in testicular tissue, whereas the longer transcripts of 2.4 and 4.4
kb are ubiquitously expressed. The expression of
znf230 in testis is developmentally regulated and first detected at day 6 postnatal (pn). It reaches adult level between day 14 and 21 pn during which round spermatids appear in seminiferous tubule. The protein of
znf230 exhibits DNA binding activity and its ring finger domain may function as an activator module in transcription. Therefore, it is postulated that znf230 may function as a testis specific transcription factor during mouse spermatogenesis.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>Blotting, Southern</subject><subject>Blotting, Western</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - metabolism</subject><subject>DNA-Binding Proteins - chemistry</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Gene Expression Regulation</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Humans</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>Protein Binding</subject><subject>Protein Biosynthesis</subject><subject>Protein Structure, Tertiary</subject><subject>RACE</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Seminiferous Tubules - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>Southwestern blot</subject><subject>Spermatogenesis</subject><subject>Testis - metabolism</subject><subject>Time Factors</subject><subject>Tissue Distribution</subject><subject>Transcription Factors</subject><subject>Transcription, Genetic</subject><subject>Transcriptional regulation</subject><subject>Two-Hybrid System Techniques</subject><subject>Yeast one-hybridization</subject><subject>znf230 gene</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1LxDAQhoMo7vrxE5ScRA_VSdM2zUlk8QsUDyp4C9l0opG2WZNW0F9v6y563MvMYZ53ZnhfQg4YnDJgxdkjABRJKtnLMfATACkgERtkykBCkjLINsn0D5mQnRjfARjLCrlNJiwtIcsLOSX63tdo-loHamrfuvaV6rai5k0HbToM7lt3zrfUW6pp4_uINC4wNLrzr9hidDEJWOsOKxpGsR0KBjrO6HdrUw57ZMvqOuL-qu-S56vLp9lNcvdwfTu7uEtMlpddoqVMZVqizdncVHwOlRWmSgVoZNYWgBpKNs8sIlqRS2Mz5MJyoXMDJkfOd8nRcu8i-I8eY6caFw3WtW5x-FsJzgWDolwLslKUJWdiAPMlaIKPMaBVi-AaHb4UAzWGoH5DUKPDCrj6DUGNusPVgX7eYPWvWrk-AOdLAAc_Ph0GFY3D1mDlAppOVd6tOfED8NuYyw</recordid><startdate>20030627</startdate><enddate>20030627</enddate><creator>Qiu, Weimin</creator><creator>Zhang, Sizhong</creator><creator>Xiao, Cuiying</creator><creator>Xu, Wenmin</creator><creator>Ma, Yongxin</creator><creator>Liu, Yunqiang</creator><creator>Wu, Qiaqing</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030627</creationdate><title>Molecular cloning and characterization of a mouse spermatogenesis-related ring finger gene znf230</title><author>Qiu, Weimin ; Zhang, Sizhong ; Xiao, Cuiying ; Xu, Wenmin ; Ma, Yongxin ; Liu, Yunqiang ; Wu, Qiaqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-a992928ef51bcd3b0df7cd270ae1ff60ea081b4feeef759cf4e37f37a5c0c5e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>Blotting, Southern</topic><topic>Blotting, Western</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - metabolism</topic><topic>DNA-Binding Proteins - chemistry</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Gene Expression Regulation</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Humans</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Open Reading Frames</topic><topic>Protein Binding</topic><topic>Protein Biosynthesis</topic><topic>Protein Structure, Tertiary</topic><topic>RACE</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Seminiferous Tubules - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>Southwestern blot</topic><topic>Spermatogenesis</topic><topic>Testis - metabolism</topic><topic>Time Factors</topic><topic>Tissue Distribution</topic><topic>Transcription Factors</topic><topic>Transcription, Genetic</topic><topic>Transcriptional regulation</topic><topic>Two-Hybrid System Techniques</topic><topic>Yeast one-hybridization</topic><topic>znf230 gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qiu, Weimin</creatorcontrib><creatorcontrib>Zhang, Sizhong</creatorcontrib><creatorcontrib>Xiao, Cuiying</creatorcontrib><creatorcontrib>Xu, Wenmin</creatorcontrib><creatorcontrib>Ma, Yongxin</creatorcontrib><creatorcontrib>Liu, Yunqiang</creatorcontrib><creatorcontrib>Wu, Qiaqing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiu, Weimin</au><au>Zhang, Sizhong</au><au>Xiao, Cuiying</au><au>Xu, Wenmin</au><au>Ma, Yongxin</au><au>Liu, Yunqiang</au><au>Wu, Qiaqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and characterization of a mouse spermatogenesis-related ring finger gene znf230</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2003-06-27</date><risdate>2003</risdate><volume>306</volume><issue>2</issue><spage>347</spage><epage>353</epage><pages>347-353</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Complete cDNA of mouse gene
znf230 was cloned by rapid amplification of cDNA ends (RACE). This cDNA is 982 base pairs (bp) in length and encodes a 230 amino acids (aa) protein with a ring finger domain at its C-terminus. Ninety-one and 98% nucleotide (nt) and aa sequence identity are observed with its human homolog. Revealed by Northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR), this cDNA is only detected in testicular tissue, whereas the longer transcripts of 2.4 and 4.4
kb are ubiquitously expressed. The expression of
znf230 in testis is developmentally regulated and first detected at day 6 postnatal (pn). It reaches adult level between day 14 and 21 pn during which round spermatids appear in seminiferous tubule. The protein of
znf230 exhibits DNA binding activity and its ring finger domain may function as an activator module in transcription. Therefore, it is postulated that znf230 may function as a testis specific transcription factor during mouse spermatogenesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12804569</pmid><doi>10.1016/S0006-291X(03)00970-7</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Southern Blotting, Western Cloning, Molecular DNA, Complementary - metabolism DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics Gene Expression Regulation Gene Expression Regulation, Developmental Humans Male Mice Molecular Sequence Data Open Reading Frames Protein Binding Protein Biosynthesis Protein Structure, Tertiary RACE Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Seminiferous Tubules - metabolism Sequence Homology, Amino Acid Southwestern blot Spermatogenesis Testis - metabolism Time Factors Tissue Distribution Transcription Factors Transcription, Genetic Transcriptional regulation Two-Hybrid System Techniques Yeast one-hybridization znf230 gene |
| title | Molecular cloning and characterization of a mouse spermatogenesis-related ring finger gene znf230 |
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