Bovine NALP5, NALP8, and NALP9 Genes: Assignment to a QTL Region and the Expression in Adult Tissues, Oocytes, and Preimplantation Embryos
A 3204-bp full-length cDNA of bovine NALP9 was cloned and its genomic organization was analyzed. The 2988-bp open reading frame covers 9 exons and encodes a deduced protein of 996 amino acids containing Pyrin, Nacht and leucine-rich repeat domains like the human NALP gene family members. Mapping wit...
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| Veröffentlicht in: | Biology of reproduction 2006-03, Vol.74 (3), p.577-584 |
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| creator | Ponsuksili, Siriluck Brunner, Ronald M Goldammer, Tom Kühn, Christa Walz, Christina Chomdej, Siriwadee Tesfaye, Dawit Schellander, Karl Wimmers, Klaus Schwerin, Manfred |
| description | A 3204-bp full-length cDNA of bovine NALP9 was cloned and its genomic organization was analyzed. The 2988-bp open reading frame covers 9 exons and encodes a deduced protein of 996 amino acids containing Pyrin, Nacht and leucine-rich repeat domains like the human NALP gene family members. Mapping with the WGRH5000 panel and fluorescence in situ hybridization assigned NALP9 in close vicinity to BM2078 (LOD score 25.71; distance 0.0 cR5000) on bovine chromosome 18, BTA18q25-q26, within a previously identified QTL region for reproductive traits flanked by the bovine marker BM2078 and TGLA227. BAC contig analysis revealed that NALP9, NALP8, and NALP5 map in this QTL region. Temporospatial expression of these members of the NALP gene family was monitored. Among the adult tissues examined, transcripts of NALP8 and NALP9 were detected exclusively in testis and ovary, whereas transcripts of the NALP5 gene are limited to the ovary. The transcripts of NALP9, NALP8, and NALP5 were detected in oocytes before and after in vitro maturation and with a gradual decline from 2-cell to 8-cell stage, suggesting no reactivation at the time of bovine maternal to embryonic transition. Assignment to a QTL region for reproductive traits and preferential expression of NALP9, NALP8, and NALP5 in oocyte, germinal lineage, and gonad cells may suggest their functional relevance to reproduction and possible contribution to phenotypic variation. |
| doi_str_mv | 10.1095/biolreprod.105.045096 |
| format | Article |
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The 2988-bp open reading frame covers 9 exons and encodes a deduced protein of 996 amino acids containing Pyrin, Nacht and leucine-rich repeat domains like the human NALP gene family members. Mapping with the WGRH5000 panel and fluorescence in situ hybridization assigned NALP9 in close vicinity to BM2078 (LOD score 25.71; distance 0.0 cR5000) on bovine chromosome 18, BTA18q25-q26, within a previously identified QTL region for reproductive traits flanked by the bovine marker BM2078 and TGLA227. BAC contig analysis revealed that NALP9, NALP8, and NALP5 map in this QTL region. Temporospatial expression of these members of the NALP gene family was monitored. Among the adult tissues examined, transcripts of NALP8 and NALP9 were detected exclusively in testis and ovary, whereas transcripts of the NALP5 gene are limited to the ovary. The transcripts of NALP9, NALP8, and NALP5 were detected in oocytes before and after in vitro maturation and with a gradual decline from 2-cell to 8-cell stage, suggesting no reactivation at the time of bovine maternal to embryonic transition. Assignment to a QTL region for reproductive traits and preferential expression of NALP9, NALP8, and NALP5 in oocyte, germinal lineage, and gonad cells may suggest their functional relevance to reproduction and possible contribution to phenotypic variation.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.105.045096</identifier><identifier>PMID: 16339045</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction, Inc</publisher><subject>Age Factors ; Amino Acid Sequence ; Animals ; Apoptosis Regulatory Proteins - genetics ; Autoantigens - genetics ; Biological and medical sciences ; Blastocyst - physiology ; Cattle ; Contents ; early development ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental - physiology ; Male ; Mammalian female genital system ; Molecular Sequence Data ; Morphology. Physiology ; Multigene Family - physiology ; oocyte development ; Oocytes - physiology ; ovary ; Ovary - physiology ; Phenotype ; Pregnancy ; Quantitative Trait Loci ; Reproduction - physiology ; RNA, Messenger - metabolism ; Sequence Homology, Amino Acid ; testis ; Testis - physiology ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2006-03, Vol.74 (3), p.577-584</ispartof><rights>Society for the Study of Reproduction</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b533t-7e4d51b8efad23d80591431fe854e4d0064a435d72ded37b7e72814646601b103</citedby><cites>FETCH-LOGICAL-b533t-7e4d51b8efad23d80591431fe854e4d0064a435d72ded37b7e72814646601b103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1095/biolreprod.105.045096$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,776,780,26957,27903,27904,52341</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17556478$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16339045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ponsuksili, Siriluck</creatorcontrib><creatorcontrib>Brunner, Ronald M</creatorcontrib><creatorcontrib>Goldammer, Tom</creatorcontrib><creatorcontrib>Kühn, Christa</creatorcontrib><creatorcontrib>Walz, Christina</creatorcontrib><creatorcontrib>Chomdej, Siriwadee</creatorcontrib><creatorcontrib>Tesfaye, Dawit</creatorcontrib><creatorcontrib>Schellander, Karl</creatorcontrib><creatorcontrib>Wimmers, Klaus</creatorcontrib><creatorcontrib>Schwerin, Manfred</creatorcontrib><title>Bovine NALP5, NALP8, and NALP9 Genes: Assignment to a QTL Region and the Expression in Adult Tissues, Oocytes, and Preimplantation Embryos</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>A 3204-bp full-length cDNA of bovine NALP9 was cloned and its genomic organization was analyzed. The 2988-bp open reading frame covers 9 exons and encodes a deduced protein of 996 amino acids containing Pyrin, Nacht and leucine-rich repeat domains like the human NALP gene family members. Mapping with the WGRH5000 panel and fluorescence in situ hybridization assigned NALP9 in close vicinity to BM2078 (LOD score 25.71; distance 0.0 cR5000) on bovine chromosome 18, BTA18q25-q26, within a previously identified QTL region for reproductive traits flanked by the bovine marker BM2078 and TGLA227. BAC contig analysis revealed that NALP9, NALP8, and NALP5 map in this QTL region. Temporospatial expression of these members of the NALP gene family was monitored. Among the adult tissues examined, transcripts of NALP8 and NALP9 were detected exclusively in testis and ovary, whereas transcripts of the NALP5 gene are limited to the ovary. The transcripts of NALP9, NALP8, and NALP5 were detected in oocytes before and after in vitro maturation and with a gradual decline from 2-cell to 8-cell stage, suggesting no reactivation at the time of bovine maternal to embryonic transition. Assignment to a QTL region for reproductive traits and preferential expression of NALP9, NALP8, and NALP5 in oocyte, germinal lineage, and gonad cells may suggest their functional relevance to reproduction and possible contribution to phenotypic variation.</description><subject>Age Factors</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Apoptosis Regulatory Proteins - genetics</subject><subject>Autoantigens - genetics</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - physiology</subject><subject>Cattle</subject><subject>Contents</subject><subject>early development</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Male</subject><subject>Mammalian female genital system</subject><subject>Molecular Sequence Data</subject><subject>Morphology. Physiology</subject><subject>Multigene Family - physiology</subject><subject>oocyte development</subject><subject>Oocytes - physiology</subject><subject>ovary</subject><subject>Ovary - physiology</subject><subject>Phenotype</subject><subject>Pregnancy</subject><subject>Quantitative Trait Loci</subject><subject>Reproduction - physiology</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>testis</subject><subject>Testis - physiology</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-O0zAQxiMEYsvCIwC-AJd2seN_CbeyKgtSxS7QPVtOMmmNkrhrp5S-Ak_NpKnUG-I0Y_s342_mS5KXjF4xmsv3hfNNgG3wFZ7lFRWS5upRMmEyzWc6VdnjZEIpVTPOFb9InsX4k1ImeMqfJhdMcZ5jyST589H_ch2Qr_PlnZweQzYltquOaU5uoIP4gcxjdOuuha4nvSeWfFstyXdYO98d2X4DZPF7GwAxvHIdmVe7picrF-MO4pTc-vLQD8lA3wVw7baxXW_7AV-0RTj4-Dx5UtsmwotTvEzuPy1W159ny9ubL9fz5ayQnPczDaKSrMigtlXKq4zKHMdiNWRS4BOOLKzgstJpBRXXhQadZkwooRRlBaP8Mnk39sXlPaC63rQultCgIPC7aDTnGjecKSTf_pNkmjGlZY6gHMEy-BgD1GYbXGvDwTBqBrvM2S48SzPahXWvTh_sihaqc9XJHwTenAAbS9vUwXali2dOS6mEzs7cxq03exfAxNY2DbblZr_fa2G4kVoj93rkauuNXQfsdf8jpYxTVKWEGDrxkUDFvoP_HOQvYpXFVQ</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Ponsuksili, Siriluck</creator><creator>Brunner, Ronald M</creator><creator>Goldammer, Tom</creator><creator>Kühn, Christa</creator><creator>Walz, Christina</creator><creator>Chomdej, Siriwadee</creator><creator>Tesfaye, Dawit</creator><creator>Schellander, Karl</creator><creator>Wimmers, Klaus</creator><creator>Schwerin, Manfred</creator><general>Society for the Study of Reproduction, Inc</general><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060301</creationdate><title>Bovine NALP5, NALP8, and NALP9 Genes: Assignment to a QTL Region and the Expression in Adult Tissues, Oocytes, and Preimplantation Embryos</title><author>Ponsuksili, Siriluck ; Brunner, Ronald M ; Goldammer, Tom ; Kühn, Christa ; Walz, Christina ; Chomdej, Siriwadee ; Tesfaye, Dawit ; Schellander, Karl ; Wimmers, Klaus ; Schwerin, Manfred</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b533t-7e4d51b8efad23d80591431fe854e4d0064a435d72ded37b7e72814646601b103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Age Factors</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Apoptosis Regulatory Proteins - genetics</topic><topic>Autoantigens - genetics</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - physiology</topic><topic>Cattle</topic><topic>Contents</topic><topic>early development</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Male</topic><topic>Mammalian female genital system</topic><topic>Molecular Sequence Data</topic><topic>Morphology. Physiology</topic><topic>Multigene Family - physiology</topic><topic>oocyte development</topic><topic>Oocytes - physiology</topic><topic>ovary</topic><topic>Ovary - physiology</topic><topic>Phenotype</topic><topic>Pregnancy</topic><topic>Quantitative Trait Loci</topic><topic>Reproduction - physiology</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>testis</topic><topic>Testis - physiology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ponsuksili, Siriluck</creatorcontrib><creatorcontrib>Brunner, Ronald M</creatorcontrib><creatorcontrib>Goldammer, Tom</creatorcontrib><creatorcontrib>Kühn, Christa</creatorcontrib><creatorcontrib>Walz, Christina</creatorcontrib><creatorcontrib>Chomdej, Siriwadee</creatorcontrib><creatorcontrib>Tesfaye, Dawit</creatorcontrib><creatorcontrib>Schellander, Karl</creatorcontrib><creatorcontrib>Wimmers, Klaus</creatorcontrib><creatorcontrib>Schwerin, Manfred</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ponsuksili, Siriluck</au><au>Brunner, Ronald M</au><au>Goldammer, Tom</au><au>Kühn, Christa</au><au>Walz, Christina</au><au>Chomdej, Siriwadee</au><au>Tesfaye, Dawit</au><au>Schellander, Karl</au><au>Wimmers, Klaus</au><au>Schwerin, Manfred</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine NALP5, NALP8, and NALP9 Genes: Assignment to a QTL Region and the Expression in Adult Tissues, Oocytes, and Preimplantation Embryos</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2006-03-01</date><risdate>2006</risdate><volume>74</volume><issue>3</issue><spage>577</spage><epage>584</epage><pages>577-584</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>A 3204-bp full-length cDNA of bovine NALP9 was cloned and its genomic organization was analyzed. The 2988-bp open reading frame covers 9 exons and encodes a deduced protein of 996 amino acids containing Pyrin, Nacht and leucine-rich repeat domains like the human NALP gene family members. Mapping with the WGRH5000 panel and fluorescence in situ hybridization assigned NALP9 in close vicinity to BM2078 (LOD score 25.71; distance 0.0 cR5000) on bovine chromosome 18, BTA18q25-q26, within a previously identified QTL region for reproductive traits flanked by the bovine marker BM2078 and TGLA227. BAC contig analysis revealed that NALP9, NALP8, and NALP5 map in this QTL region. Temporospatial expression of these members of the NALP gene family was monitored. Among the adult tissues examined, transcripts of NALP8 and NALP9 were detected exclusively in testis and ovary, whereas transcripts of the NALP5 gene are limited to the ovary. The transcripts of NALP9, NALP8, and NALP5 were detected in oocytes before and after in vitro maturation and with a gradual decline from 2-cell to 8-cell stage, suggesting no reactivation at the time of bovine maternal to embryonic transition. Assignment to a QTL region for reproductive traits and preferential expression of NALP9, NALP8, and NALP5 in oocyte, germinal lineage, and gonad cells may suggest their functional relevance to reproduction and possible contribution to phenotypic variation.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction, Inc</pub><pmid>16339045</pmid><doi>10.1095/biolreprod.105.045096</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2006-03, Vol.74 (3), p.577-584 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; BioOne Complete |
| subjects | Age Factors Amino Acid Sequence Animals Apoptosis Regulatory Proteins - genetics Autoantigens - genetics Biological and medical sciences Blastocyst - physiology Cattle Contents early development Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental - physiology Male Mammalian female genital system Molecular Sequence Data Morphology. Physiology Multigene Family - physiology oocyte development Oocytes - physiology ovary Ovary - physiology Phenotype Pregnancy Quantitative Trait Loci Reproduction - physiology RNA, Messenger - metabolism Sequence Homology, Amino Acid testis Testis - physiology Vertebrates: reproduction |
| title | Bovine NALP5, NALP8, and NALP9 Genes: Assignment to a QTL Region and the Expression in Adult Tissues, Oocytes, and Preimplantation Embryos |
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