Antioxidant enzymatically modified isoquercitrin suppresses the development of liver preneoplastic lesions in rats induced by β-naphthoflavone
Abstract To investigate the modifying effect of enzymatically modified isoquercitrin (EMIQ) on hepatocellular tumor promotion induced by β-naphthoflavone (BNF) treatment, male rats were administered a single intraperitoneal injection of N -diethylnitrosamine (DEN) and were fed a diet containing BNF...
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| Veröffentlicht in: | Toxicology (Amsterdam) 2010-02, Vol.268 (3), p.213-218 |
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| creator | Shimada, Yuko Dewa, Yasuaki Ichimura, Ryohei Suzuki, Terumasa Mizukami, Sayaka Hayashi, Shim-mo Shibutani, Makoto Mitsumori, Kunitoshi |
| description | Abstract To investigate the modifying effect of enzymatically modified isoquercitrin (EMIQ) on hepatocellular tumor promotion induced by β-naphthoflavone (BNF) treatment, male rats were administered a single intraperitoneal injection of N -diethylnitrosamine (DEN) and were fed a diet containing BNF (0.5%) for 6 weeks with or without EMIQ (0.2%) in the drinking water after DEN initiation. One week after the commencement of the administration of BNF, rats were subjected to a two-thirds partial hepatectomy. The number and area of GST-P positive foci, the number of COX2-positive cells and the area of elastica-van Gieson (EVG)-positive connective tissue fibers promoted by BNF were significantly suppressed by the administration of the antioxidant EMIQ. Real-time RT-PCR analysis revealed that EMIQ treatment decreased mRNA expression levels of Gstm1 , Serpine1 , Cox2 and Nfkbia and increased mRNA expression levels of Yc2 compared with those in the DEN–BNF group. These results suggest that co-administration of EMIQ suppresses the hepatocellular tumor-promoting activity of BNF in rats through the anti-inflammatory effects of EMIQ and restores the cellular redox balance altered by BNF. |
| doi_str_mv | 10.1016/j.tox.2009.12.019 |
| format | Article |
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One week after the commencement of the administration of BNF, rats were subjected to a two-thirds partial hepatectomy. The number and area of GST-P positive foci, the number of COX2-positive cells and the area of elastica-van Gieson (EVG)-positive connective tissue fibers promoted by BNF were significantly suppressed by the administration of the antioxidant EMIQ. Real-time RT-PCR analysis revealed that EMIQ treatment decreased mRNA expression levels of Gstm1 , Serpine1 , Cox2 and Nfkbia and increased mRNA expression levels of Yc2 compared with those in the DEN–BNF group. These results suggest that co-administration of EMIQ suppresses the hepatocellular tumor-promoting activity of BNF in rats through the anti-inflammatory effects of EMIQ and restores the cellular redox balance altered by BNF.</description><identifier>ISSN: 0300-483X</identifier><identifier>EISSN: 1879-3185</identifier><identifier>DOI: 10.1016/j.tox.2009.12.019</identifier><identifier>PMID: 20045035</identifier><identifier>CODEN: TXICDD</identifier><language>eng</language><publisher>Kidlington: Elsevier Ireland Ltd</publisher><subject>Animals ; Anticarcinogenic Agents ; Antioxidants - chemistry ; Antioxidants - pharmacology ; beta-Naphthoflavone - antagonists & inhibitors ; beta-Naphthoflavone - toxicity ; Biological and medical sciences ; Connective Tissue - drug effects ; Connective Tissue - metabolism ; Cyclooxygenase 2 - metabolism ; Emergency ; Enzymatically modified isoquercitrin ; Glutathione Peroxidase - metabolism ; Hepatocellular tumor promotion ; Immunohistochemistry ; Inflammation ; Liver Neoplasms, Experimental - chemically induced ; Liver Neoplasms, Experimental - pathology ; Male ; Medical sciences ; Oxidative stress ; Precancerous Conditions - pathology ; Precancerous Conditions - prevention & control ; Quercetin - analogs & derivatives ; Quercetin - chemistry ; Quercetin - pharmacology ; Rats ; Rats, Inbred F344 ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Toxicology ; β-Naphthoflavone</subject><ispartof>Toxicology (Amsterdam), 2010-02, Vol.268 (3), p.213-218</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2009 Elsevier Ireland Ltd</rights><rights>2015 INIST-CNRS</rights><rights>(c) 2009 Elsevier Ireland Ltd. 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One week after the commencement of the administration of BNF, rats were subjected to a two-thirds partial hepatectomy. The number and area of GST-P positive foci, the number of COX2-positive cells and the area of elastica-van Gieson (EVG)-positive connective tissue fibers promoted by BNF were significantly suppressed by the administration of the antioxidant EMIQ. Real-time RT-PCR analysis revealed that EMIQ treatment decreased mRNA expression levels of Gstm1 , Serpine1 , Cox2 and Nfkbia and increased mRNA expression levels of Yc2 compared with those in the DEN–BNF group. These results suggest that co-administration of EMIQ suppresses the hepatocellular tumor-promoting activity of BNF in rats through the anti-inflammatory effects of EMIQ and restores the cellular redox balance altered by BNF.</description><subject>Animals</subject><subject>Anticarcinogenic Agents</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - pharmacology</subject><subject>beta-Naphthoflavone - antagonists & inhibitors</subject><subject>beta-Naphthoflavone - toxicity</subject><subject>Biological and medical sciences</subject><subject>Connective Tissue - drug effects</subject><subject>Connective Tissue - metabolism</subject><subject>Cyclooxygenase 2 - metabolism</subject><subject>Emergency</subject><subject>Enzymatically modified isoquercitrin</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Hepatocellular tumor promotion</subject><subject>Immunohistochemistry</subject><subject>Inflammation</subject><subject>Liver Neoplasms, Experimental - chemically induced</subject><subject>Liver Neoplasms, Experimental - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Oxidative stress</subject><subject>Precancerous Conditions - pathology</subject><subject>Precancerous Conditions - prevention & control</subject><subject>Quercetin - analogs & derivatives</subject><subject>Quercetin - chemistry</subject><subject>Quercetin - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Toxicology</subject><subject>β-Naphthoflavone</subject><issn>0300-483X</issn><issn>1879-3185</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9ks-q1DAUxoso3vHqA7iRbMRVx5OmTacIwuXiP7jgQgV3IU1OmYxpUpN0uPUlfBcfxGcyZUYFF67O5vd9nPN9pygeU9hSoPz5YZv87bYC6La02gLt7hQbumu7ktFdc7fYAAMo6x37fFE8iPEAABWr-f3iIkvqBlizKb5fuWT8rdHSJYLu2zLKZJS0diGj12YwqImJ_uuMQZkUjCNxnqaAMWIkaY9E4xGtn0bMej8Qa44YSAYc-snKmM2IxWi8iySLg0zr1LPKvv1Cfv4onZz2ae8HK4_e4cPi3iBtxEfneVl8ev3q4_Xb8ub9m3fXVzelqlmbyqHuVS8167Fv8sG65brFBnjHdD-ArNtaIzQdKtXwrlJKsa7mcoBO80oqzthl8ezkO4X1uJjEaKJCa2VefI6iZawF3kCdSXoiVfAxBhzEFMwowyIoiLUGcRC5BrHWIGglcg1Z8-TsPvcj6j-K37ln4OkZkDGnPQTplIl_uapZnVejFycOcxZHg0FEZdDl9ExAlYT25r9rvPxHraxxa71fcMF48HNwOWRBRcwC8WH9l_VdoAPgNeXsF-_EwI4</recordid><startdate>20100209</startdate><enddate>20100209</enddate><creator>Shimada, Yuko</creator><creator>Dewa, Yasuaki</creator><creator>Ichimura, Ryohei</creator><creator>Suzuki, Terumasa</creator><creator>Mizukami, Sayaka</creator><creator>Hayashi, Shim-mo</creator><creator>Shibutani, Makoto</creator><creator>Mitsumori, Kunitoshi</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100209</creationdate><title>Antioxidant enzymatically modified isoquercitrin suppresses the development of liver preneoplastic lesions in rats induced by β-naphthoflavone</title><author>Shimada, Yuko ; 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One week after the commencement of the administration of BNF, rats were subjected to a two-thirds partial hepatectomy. The number and area of GST-P positive foci, the number of COX2-positive cells and the area of elastica-van Gieson (EVG)-positive connective tissue fibers promoted by BNF were significantly suppressed by the administration of the antioxidant EMIQ. Real-time RT-PCR analysis revealed that EMIQ treatment decreased mRNA expression levels of Gstm1 , Serpine1 , Cox2 and Nfkbia and increased mRNA expression levels of Yc2 compared with those in the DEN–BNF group. These results suggest that co-administration of EMIQ suppresses the hepatocellular tumor-promoting activity of BNF in rats through the anti-inflammatory effects of EMIQ and restores the cellular redox balance altered by BNF.</abstract><cop>Kidlington</cop><pub>Elsevier Ireland Ltd</pub><pmid>20045035</pmid><doi>10.1016/j.tox.2009.12.019</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Anticarcinogenic Agents Antioxidants - chemistry Antioxidants - pharmacology beta-Naphthoflavone - antagonists & inhibitors beta-Naphthoflavone - toxicity Biological and medical sciences Connective Tissue - drug effects Connective Tissue - metabolism Cyclooxygenase 2 - metabolism Emergency Enzymatically modified isoquercitrin Glutathione Peroxidase - metabolism Hepatocellular tumor promotion Immunohistochemistry Inflammation Liver Neoplasms, Experimental - chemically induced Liver Neoplasms, Experimental - pathology Male Medical sciences Oxidative stress Precancerous Conditions - pathology Precancerous Conditions - prevention & control Quercetin - analogs & derivatives Quercetin - chemistry Quercetin - pharmacology Rats Rats, Inbred F344 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RNA, Messenger - genetics Toxicology β-Naphthoflavone |
| title | Antioxidant enzymatically modified isoquercitrin suppresses the development of liver preneoplastic lesions in rats induced by β-naphthoflavone |
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