Development of in situ hybridization assay that differentiates between two genotypes of Porcine circovirus-2 in formalin-fixed, paraffin-embedded tissues
The aim of the current study was to develop a nonradioactive in situ hybridization assay that can differentiate between genotypes 2a and 2b of Porcine circovirus-2 (PCV-2) in formalin-fixed, paraffin-embedded lymph node tissues from pigs with postweaning multisystemic wasting syndrome. Two different...
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| Veröffentlicht in: | Journal of veterinary diagnostic investigation 2010-03, Vol.22 (2), p.231-233 |
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| container_title | Journal of veterinary diagnostic investigation |
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| creator | Kim, Duyeol Ha, Yooncheol Oh, Yeonsu Han, Kiwon Noh, Seung Ho Kim, Cheolwoo Kim, Sung-Hoon Chae, Chanhee |
| description | The aim of the current study was to develop a nonradioactive in situ hybridization assay that can differentiate between genotypes 2a and 2b of Porcine circovirus-2 (PCV-2) in formalin-fixed, paraffin-embedded lymph node tissues from pigs with postweaning multisystemic wasting syndrome. Two different digoxigenin-labeled oligonucleotide probes were designed from the PCV-2 open reading frame 2 sequences. The PCV-2a–specific probe did not hybridize with formalin-fixed, paraffin-embedded lymph nodes from naturally PCV-2b–infected pigs and vice versa. Both PCV-2a–specific and PCV-2b–specific probes gave consistent negative signals in lymph nodes from naturally PCV-1–infected pigs. The in situ hybridization assay described in the present study represents a diagnostic tool that can differentiate between the 2 genotypes of PCV-2. |
| doi_str_mv | 10.1177/104063871002200209 |
| format | Article |
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Two different digoxigenin-labeled oligonucleotide probes were designed from the PCV-2 open reading frame 2 sequences. The PCV-2a–specific probe did not hybridize with formalin-fixed, paraffin-embedded lymph nodes from naturally PCV-2b–infected pigs and vice versa. Both PCV-2a–specific and PCV-2b–specific probes gave consistent negative signals in lymph nodes from naturally PCV-1–infected pigs. The in situ hybridization assay described in the present study represents a diagnostic tool that can differentiate between the 2 genotypes of PCV-2.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/104063871002200209</identifier><identifier>PMID: 20224081</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Circovirus - classification ; Circovirus - genetics ; Circovirus - isolation & purification ; diagnostic techniques ; disease diagnosis ; Formaldehyde - chemistry ; formalin-fixed, paraffin-embedded tissues ; Genotype ; In Situ Hybridization - methods ; In Situ Hybridization - veterinary ; lymph nodes ; molecular sequence data ; nucleic acid hybridization ; nucleotide sequences ; open reading frames ; Paraffin Embedding - veterinary ; pathotypes ; Porcine circovirus ; Porcine Postweaning Multisystemic Wasting Syndrome - virology ; postweaning multisystemic wasting syndrome ; strain differences ; Swine ; swine diseases ; Tissue Fixation - veterinary ; vertebrate viruses</subject><ispartof>Journal of veterinary diagnostic investigation, 2010-03, Vol.22 (2), p.231-233</ispartof><rights>2010 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-c1c4908fe5356f4c043535c107435db090d0762ba10f3b51bcd726d47ff69263</citedby><cites>FETCH-LOGICAL-c410t-c1c4908fe5356f4c043535c107435db090d0762ba10f3b51bcd726d47ff69263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/104063871002200209$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/104063871002200209$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20224081$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Duyeol</creatorcontrib><creatorcontrib>Ha, Yooncheol</creatorcontrib><creatorcontrib>Oh, Yeonsu</creatorcontrib><creatorcontrib>Han, Kiwon</creatorcontrib><creatorcontrib>Noh, Seung Ho</creatorcontrib><creatorcontrib>Kim, Cheolwoo</creatorcontrib><creatorcontrib>Kim, Sung-Hoon</creatorcontrib><creatorcontrib>Chae, Chanhee</creatorcontrib><title>Development of in situ hybridization assay that differentiates between two genotypes of Porcine circovirus-2 in formalin-fixed, paraffin-embedded tissues</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>The aim of the current study was to develop a nonradioactive in situ hybridization assay that can differentiate between genotypes 2a and 2b of Porcine circovirus-2 (PCV-2) in formalin-fixed, paraffin-embedded lymph node tissues from pigs with postweaning multisystemic wasting syndrome. Two different digoxigenin-labeled oligonucleotide probes were designed from the PCV-2 open reading frame 2 sequences. The PCV-2a–specific probe did not hybridize with formalin-fixed, paraffin-embedded lymph nodes from naturally PCV-2b–infected pigs and vice versa. Both PCV-2a–specific and PCV-2b–specific probes gave consistent negative signals in lymph nodes from naturally PCV-1–infected pigs. The in situ hybridization assay described in the present study represents a diagnostic tool that can differentiate between the 2 genotypes of PCV-2.</description><subject>Animals</subject><subject>Circovirus - classification</subject><subject>Circovirus - genetics</subject><subject>Circovirus - isolation & purification</subject><subject>diagnostic techniques</subject><subject>disease diagnosis</subject><subject>Formaldehyde - chemistry</subject><subject>formalin-fixed, paraffin-embedded tissues</subject><subject>Genotype</subject><subject>In Situ Hybridization - methods</subject><subject>In Situ Hybridization - veterinary</subject><subject>lymph nodes</subject><subject>molecular sequence data</subject><subject>nucleic acid hybridization</subject><subject>nucleotide sequences</subject><subject>open reading frames</subject><subject>Paraffin Embedding - veterinary</subject><subject>pathotypes</subject><subject>Porcine circovirus</subject><subject>Porcine Postweaning Multisystemic Wasting Syndrome - virology</subject><subject>postweaning multisystemic wasting syndrome</subject><subject>strain differences</subject><subject>Swine</subject><subject>swine diseases</subject><subject>Tissue Fixation - veterinary</subject><subject>vertebrate viruses</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhSMEoqXwAizAOzaEXv8knixRKT9SJZAoa8uxr6euEjvYTtvhTXhbPExhg8TC8rH1nXNtnaZ5TuENpVKeUhDQ842kAIzVBcOD5pgOgrdi4P3DqivQ7omj5knO1wAd6yR93ByxahCwocfNz3d4g1NcZgyFREd8INmXlVztxuSt_6GLj4HonPWOlCtdiPXOYaq01wUzGbHcIgZSbiPZYohlt9TbGvQlJuMDEuOTiTc-rbll-3QX06wnH1rn79C-JotO2rl6xnlEa9GS4nNeMT9tHjk9ZXx2v580l-_PL88-thefP3w6e3vRGkGhtIYaMcDGYce73gkDgldlKMgq7AgDWJA9GzUFx8eOjsZK1lshnesH1vOT5tUhdknxex1b1OyzwWnSAeOaleRcAge6qSQ7kCbFnBM6tSQ_67RTFNS-EPVvIdX04j5-HWe0fy1_GqjA6QHIeovqOq4p1N_-P_LlweF0VHqbfFbfvjKgvx_ZM9HzXwFTnsw</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Kim, Duyeol</creator><creator>Ha, Yooncheol</creator><creator>Oh, Yeonsu</creator><creator>Han, Kiwon</creator><creator>Noh, Seung Ho</creator><creator>Kim, Cheolwoo</creator><creator>Kim, Sung-Hoon</creator><creator>Chae, Chanhee</creator><general>SAGE Publications</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100301</creationdate><title>Development of in situ hybridization assay that differentiates between two genotypes of Porcine circovirus-2 in formalin-fixed, paraffin-embedded tissues</title><author>Kim, Duyeol ; Ha, Yooncheol ; Oh, Yeonsu ; Han, Kiwon ; Noh, Seung Ho ; Kim, Cheolwoo ; Kim, Sung-Hoon ; Chae, Chanhee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-c1c4908fe5356f4c043535c107435db090d0762ba10f3b51bcd726d47ff69263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Circovirus - classification</topic><topic>Circovirus - genetics</topic><topic>Circovirus - isolation & purification</topic><topic>diagnostic techniques</topic><topic>disease diagnosis</topic><topic>Formaldehyde - chemistry</topic><topic>formalin-fixed, paraffin-embedded tissues</topic><topic>Genotype</topic><topic>In Situ Hybridization - methods</topic><topic>In Situ Hybridization - veterinary</topic><topic>lymph nodes</topic><topic>molecular sequence data</topic><topic>nucleic acid hybridization</topic><topic>nucleotide sequences</topic><topic>open reading frames</topic><topic>Paraffin Embedding - veterinary</topic><topic>pathotypes</topic><topic>Porcine circovirus</topic><topic>Porcine Postweaning Multisystemic Wasting Syndrome - virology</topic><topic>postweaning multisystemic wasting syndrome</topic><topic>strain differences</topic><topic>Swine</topic><topic>swine diseases</topic><topic>Tissue Fixation - veterinary</topic><topic>vertebrate viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Duyeol</creatorcontrib><creatorcontrib>Ha, Yooncheol</creatorcontrib><creatorcontrib>Oh, Yeonsu</creatorcontrib><creatorcontrib>Han, Kiwon</creatorcontrib><creatorcontrib>Noh, Seung Ho</creatorcontrib><creatorcontrib>Kim, Cheolwoo</creatorcontrib><creatorcontrib>Kim, Sung-Hoon</creatorcontrib><creatorcontrib>Chae, Chanhee</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Duyeol</au><au>Ha, Yooncheol</au><au>Oh, Yeonsu</au><au>Han, Kiwon</au><au>Noh, Seung Ho</au><au>Kim, Cheolwoo</au><au>Kim, Sung-Hoon</au><au>Chae, Chanhee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of in situ hybridization assay that differentiates between two genotypes of Porcine circovirus-2 in formalin-fixed, paraffin-embedded tissues</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2010-03-01</date><risdate>2010</risdate><volume>22</volume><issue>2</issue><spage>231</spage><epage>233</epage><pages>231-233</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>The aim of the current study was to develop a nonradioactive in situ hybridization assay that can differentiate between genotypes 2a and 2b of Porcine circovirus-2 (PCV-2) in formalin-fixed, paraffin-embedded lymph node tissues from pigs with postweaning multisystemic wasting syndrome. Two different digoxigenin-labeled oligonucleotide probes were designed from the PCV-2 open reading frame 2 sequences. The PCV-2a–specific probe did not hybridize with formalin-fixed, paraffin-embedded lymph nodes from naturally PCV-2b–infected pigs and vice versa. Both PCV-2a–specific and PCV-2b–specific probes gave consistent negative signals in lymph nodes from naturally PCV-1–infected pigs. The in situ hybridization assay described in the present study represents a diagnostic tool that can differentiate between the 2 genotypes of PCV-2.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>20224081</pmid><doi>10.1177/104063871002200209</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; SAGE Complete; Alma/SFX Local Collection |
| subjects | Animals Circovirus - classification Circovirus - genetics Circovirus - isolation & purification diagnostic techniques disease diagnosis Formaldehyde - chemistry formalin-fixed, paraffin-embedded tissues Genotype In Situ Hybridization - methods In Situ Hybridization - veterinary lymph nodes molecular sequence data nucleic acid hybridization nucleotide sequences open reading frames Paraffin Embedding - veterinary pathotypes Porcine circovirus Porcine Postweaning Multisystemic Wasting Syndrome - virology postweaning multisystemic wasting syndrome strain differences Swine swine diseases Tissue Fixation - veterinary vertebrate viruses |
| title | Development of in situ hybridization assay that differentiates between two genotypes of Porcine circovirus-2 in formalin-fixed, paraffin-embedded tissues |
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