Nuclear Targeting of Gold Nanoparticles in Cancer Cells Induces DNA Damage, Causing Cytokinesis Arrest and Apoptosis
By properly conjugating gold nanoparticles with specific peptides, we were successful in selectively transporting them to the nuclei of cancer cells. Confocal microscopy images of DNA double-strand breaks showed that localization of gold nanoparticles at the nucleus of a cancer cell damages the DNA....
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| Veröffentlicht in: | Journal of the American Chemical Society 2010-02, Vol.132 (5), p.1517-1519 |
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| container_issue | 5 |
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| container_title | Journal of the American Chemical Society |
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| creator | Kang, Bin Mackey, Megan A El-Sayed, Mostafa A |
| description | By properly conjugating gold nanoparticles with specific peptides, we were successful in selectively transporting them to the nuclei of cancer cells. Confocal microscopy images of DNA double-strand breaks showed that localization of gold nanoparticles at the nucleus of a cancer cell damages the DNA. Gold nanoparticle dark-field imaging of live cells in real time revealed that the nuclear targeting of gold nanoparticles specifically induces cytokinesis arrest in cancer cells, where binucleate cell formation occurs after mitosis takes place. Flow cytometry results indicated that the failure to complete cell division led to programmed cell death (apoptosis) in cancer cells. These results show that gold nanoparticles localized at the nuclei of cancer cells have important implications in understanding the interaction between nanomaterials and living systems. |
| doi_str_mv | 10.1021/ja9102698 |
| format | Article |
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These results show that gold nanoparticles localized at the nuclei of cancer cells have important implications in understanding the interaction between nanomaterials and living systems.</description><subject>Amino Acid Sequence</subject><subject>Apoptosis - drug effects</subject><subject>Carcinoma, Squamous Cell - drug therapy</subject><subject>Cell Line, Tumor</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - metabolism</subject><subject>Cytokinesis - drug effects</subject><subject>DNA Damage - drug effects</subject><subject>Gold - analysis</subject><subject>Humans</subject><subject>Metal Nanoparticles - analysis</subject><subject>Mouth Neoplasms - drug therapy</subject><subject>Oligopeptides - administration & dosage</subject><subject>Oligopeptides - therapeutic use</subject><subject>Peptides - administration & dosage</subject><subject>Peptides - therapeutic use</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkEtLxDAUhYMoOj4W_gHJRkSwmkeTtsuhPmEYN7out83t0LGT1KRd-O_NMOrK1eEePg7nHkLOObvlTPC7NRRRdZHvkRlXgiWKC71PZowxkWS5lkfkOIR1PFOR80NyJBjLlRTpjIzLqekRPH0Dv8KxsyvqWvrkekOXYN0AfuwiEGhnaQm2QU9L7PtAX6yZmujfL-f0HjawwpsITGGbUH6N7qOzGLpA595jGClYQ-eDG0YXzVNy0EIf8OxHT8j748Nb-ZwsXp9eyvkiAZmzMamlQUxbVtQia4RgeY1tnbZaqczkheGcG41aS2YAUNU1mkyIJgXVSqVancoTcrXLHbz7nGKNatOFJtYHi24KVSZlFjcpdCSvd2TjXQge22rw3Qb8V8VZtd24-ts4shc_qVO9QfNH_o4agcsdAE2o1m7yNj75T9A3TOGCvA</recordid><startdate>20100210</startdate><enddate>20100210</enddate><creator>Kang, Bin</creator><creator>Mackey, Megan A</creator><creator>El-Sayed, Mostafa A</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100210</creationdate><title>Nuclear Targeting of Gold Nanoparticles in Cancer Cells Induces DNA Damage, Causing Cytokinesis Arrest and Apoptosis</title><author>Kang, Bin ; Mackey, Megan A ; El-Sayed, Mostafa A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a380t-b3dee4f09b27c2208befb4f6557d89d111d6e6630daae5bbed722c4a5f355f643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino Acid Sequence</topic><topic>Apoptosis - drug effects</topic><topic>Carcinoma, Squamous Cell - drug therapy</topic><topic>Cell Line, Tumor</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - metabolism</topic><topic>Cytokinesis - drug effects</topic><topic>DNA Damage - drug effects</topic><topic>Gold - analysis</topic><topic>Humans</topic><topic>Metal Nanoparticles - analysis</topic><topic>Mouth Neoplasms - drug therapy</topic><topic>Oligopeptides - administration & dosage</topic><topic>Oligopeptides - therapeutic use</topic><topic>Peptides - administration & dosage</topic><topic>Peptides - therapeutic use</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kang, Bin</creatorcontrib><creatorcontrib>Mackey, Megan A</creatorcontrib><creatorcontrib>El-Sayed, Mostafa A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kang, Bin</au><au>Mackey, Megan A</au><au>El-Sayed, Mostafa A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear Targeting of Gold Nanoparticles in Cancer Cells Induces DNA Damage, Causing Cytokinesis Arrest and Apoptosis</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. 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| source | MEDLINE; American Chemical Society Journals |
| subjects | Amino Acid Sequence Apoptosis - drug effects Carcinoma, Squamous Cell - drug therapy Cell Line, Tumor Cell Nucleus - drug effects Cell Nucleus - metabolism Cytokinesis - drug effects DNA Damage - drug effects Gold - analysis Humans Metal Nanoparticles - analysis Mouth Neoplasms - drug therapy Oligopeptides - administration & dosage Oligopeptides - therapeutic use Peptides - administration & dosage Peptides - therapeutic use |
| title | Nuclear Targeting of Gold Nanoparticles in Cancer Cells Induces DNA Damage, Causing Cytokinesis Arrest and Apoptosis |
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