In Vivo Fluorescent Detection of Fe-S Clusters Coordinated by Human GRX2

A major challenge to studying Fe-S cluster biosynthesis in higher eukaryotes is the lack of simple tools for imaging metallocluster binding to proteins. We describe the first fluorescent approach for in vivo detection of 2Fe2S clusters that is based upon the complementation of Venus fluorescent prot...

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Veröffentlicht in:	Chemistry & biology 2009-12, Vol.16 (12), p.1299-1308
Hauptverfasser:	Hoff, Kevin G., Culler, Stephanie J., Nguyen, Peter Q., McGuire, Ryan M., Silberg, Jonathan J., Smolke, Christina D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites Cell Line CHEMBIO Circular Dichroism Dimerization Glutaredoxins - genetics Glutaredoxins - metabolism Humans Iron - chemistry Luminescent Proteins - genetics Luminescent Proteins - metabolism Microscopy, Fluorescence Mutation PROTEINS Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism RNA Interference SIGNALING Sulfur - chemistry
Online-Zugang:	Volltext
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