Detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in Hungary
The drinking water distribution system of a hospital was investigated using standard cultivation techniques, taxon-specific PCRs targeting pathogenic bacteria, denaturing gradient gel electrophoresis, cloning and sequencing. The results obtained verify the higher sensitivity of PCR compared to culti...
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| Veröffentlicht in: | Clinical microbiology and infection 2010-01, Vol.16 (1), p.89-92 |
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| creator | ldi, T. Felfö Heéger, Z. Vargha, M. Márialigeti, K. |
| description | The drinking water distribution system of a hospital was investigated using standard cultivation techniques, taxon-specific PCRs targeting pathogenic bacteria, denaturing gradient gel electrophoresis, cloning and sequencing. The results obtained verify the higher sensitivity of PCR compared to cultivation for detecting Legionella and Pseudomonas aeruginosa. Moreover, several other opportunistic pathogenic bacteria, such as Escherichia albertii, Acinetobacter lwoffi and Corynebacterium tuberculostrearicum, were detected, emphasizing that drinking water systems, especially those with stagnant water sections, could be the source of nosocomial infections. |
| doi_str_mv | 10.1111/j.1469-0691.2009.02795.x |
| format | Article |
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Felfö</creatorcontrib><creatorcontrib>Heéger, Z.</creatorcontrib><creatorcontrib>Vargha, M.</creatorcontrib><creatorcontrib>Márialigeti, K.</creatorcontrib><title>Detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in Hungary</title><title>Clinical microbiology and infection</title><addtitle>Clin Microbiol Infect</addtitle><description>The drinking water distribution system of a hospital was investigated using standard cultivation techniques, taxon-specific PCRs targeting pathogenic bacteria, denaturing gradient gel electrophoresis, cloning and sequencing. The results obtained verify the higher sensitivity of PCR compared to cultivation for detecting Legionella and Pseudomonas aeruginosa. 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| ispartof | Clinical microbiology and infection, 2010-01, Vol.16 (1), p.89-92 |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Acinetobacter lwoffi Bacteria Bacteria - genetics Bacteria - isolation & purification Bacteria - pathogenicity Cloning and sequencing Corynebacterium Cross Infection - microbiology Cross Infection - prevention & control Drinking water Escherichia Hospitals Humans Hungary Legionella PCR detection Phylogeny Polymerase Chain Reaction Pseudomonas aeruginosa Sensitivity and Specificity Water Microbiology Water Supply - analysis Water treatment waterborne nosocomial infections |
| title | Detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in Hungary |
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