Raman tweezers and their application to the study of singly trapped eukaryotic cells
In this review the recent emergence of Raman tweezers as an analytical technique for single eukaryotic cell analysis is described. The Raman tweezer technique combines Raman spectroscopy as a diagnostic tool with optical tweezers by which means single cells can be trapped and manipulated in a laser...
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| Veröffentlicht in: | Integrative biology (Cambridge) 2009-01, Vol.1 (1), p.43-52 |
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| creator | Snook, Richard D Harvey, Timothy J Correia Faria, Elsa Gardner, Peter |
| description | In this review the recent emergence of Raman tweezers as an analytical technique for single eukaryotic cell analysis is described. The Raman tweezer technique combines Raman spectroscopy as a diagnostic tool with optical tweezers by which means single cells can be trapped and manipulated in a laser beam using a high numerical aperture imaging microscope. Necessary instrumental requirements to facilitate Raman tweezer experiments are discussed together with practical considerations such as the potential for photodamage of cells subjected to trapping and Raman excitation. Specific applications of Raman tweezers to the analysis of cancer cells, erythrocytes and lymphocytes, micro-organisms and sub-cellular components e.g.chromosomes and mitochondria are then discussed followed by a summary of the future potential of the technique for single cell analysis. |
| doi_str_mv | 10.1039/b815253e |
| format | Article |
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| ispartof | Integrative biology (Cambridge), 2009-01, Vol.1 (1), p.43-52 |
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| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Cell Separation - instrumentation Cell Separation - methods Cell Separation - trends Equipment Design Eukaryotic Cells - cytology Eukaryotic Cells - physiology Humans Optical Tweezers Spectrum Analysis, Raman - instrumentation Spectrum Analysis, Raman - methods |
| title | Raman tweezers and their application to the study of singly trapped eukaryotic cells |
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