Immunological Study of Allogeneic Mesenchymal Stem Cells during Bone Formation
Autologous mesenchymal stem cells (MSCs) are limited in their clinical application because tissue-engineered bone cannot be pre-fabricated. Allogeneic MSCs are readily available but carry the risk of transplant rejection. It is not yet clear whether allogeneic MSCs can induce a rejection response du...
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| Veröffentlicht in: | Journal of international medical research 2009-11, Vol.37 (6), p.1750-1759 |
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| container_title | Journal of international medical research |
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| creator | Guo, SQ Xu, JZ Zou, QM Jiang, DM |
| description | Autologous mesenchymal stem cells (MSCs) are limited in their clinical application because tissue-engineered bone cannot be pre-fabricated. Allogeneic MSCs are readily available but carry the risk of transplant rejection. It is not yet clear whether allogeneic MSCs can induce a rejection response during bone formation. In this study, two strains of genetically unmatched mini-pigs were used as experimental animals to study the immunological changes in MSCs in vitro and in vivo when generating bone. Mini-pig MSCs showed low immunogenicity during osteogenesis both in vitro and in vivo, indicating that allogeneic MSCs had little or no immunogenicity in osteosis. In conclusion, allogeneic MSCs are an important source of seed cells for the tissue engineering of bone. This favours the clinical application of pre-constructed tissue-engineered bone. |
| doi_str_mv | 10.1177/147323000903700611 |
| format | Article |
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Allogeneic MSCs are readily available but carry the risk of transplant rejection. It is not yet clear whether allogeneic MSCs can induce a rejection response during bone formation. In this study, two strains of genetically unmatched mini-pigs were used as experimental animals to study the immunological changes in MSCs in vitro and in vivo when generating bone. Mini-pig MSCs showed low immunogenicity during osteogenesis both in vitro and in vivo, indicating that allogeneic MSCs had little or no immunogenicity in osteosis. In conclusion, allogeneic MSCs are an important source of seed cells for the tissue engineering of bone. This favours the clinical application of pre-constructed tissue-engineered bone.</description><identifier>ISSN: 0300-0605</identifier><identifier>EISSN: 1473-2300</identifier><identifier>DOI: 10.1177/147323000903700611</identifier><identifier>PMID: 20146873</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Bone and Bones - diagnostic imaging ; Bone Marrow Cells - cytology ; CD4-Positive T-Lymphocytes - cytology ; CD8-Positive T-Lymphocytes - cytology ; Cells, Cultured ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Histocompatibility Antigens Class I - metabolism ; Histocompatibility Antigens Class II - metabolism ; Lymphocyte Culture Test, Mixed ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - immunology ; Osteogenesis - immunology ; Radiography ; Swine ; Swine, Miniature ; Transplantation, Homologous</subject><ispartof>Journal of international medical research, 2009-11, Vol.37 (6), p.1750-1759</ispartof><rights>2009 SAGE Publications</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-a08fee1da931c39a6326abd052bd5b830cbdcc928ed80fe20ec146ad57cad0013</citedby><cites>FETCH-LOGICAL-c342t-a08fee1da931c39a6326abd052bd5b830cbdcc928ed80fe20ec146ad57cad0013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/147323000903700611$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/147323000903700611$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21966,27853,27924,27925,44945,45333</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1177/147323000903700611?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20146873$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, SQ</creatorcontrib><creatorcontrib>Xu, JZ</creatorcontrib><creatorcontrib>Zou, QM</creatorcontrib><creatorcontrib>Jiang, DM</creatorcontrib><title>Immunological Study of Allogeneic Mesenchymal Stem Cells during Bone Formation</title><title>Journal of international medical research</title><addtitle>J Int Med Res</addtitle><description>Autologous mesenchymal stem cells (MSCs) are limited in their clinical application because tissue-engineered bone cannot be pre-fabricated. Allogeneic MSCs are readily available but carry the risk of transplant rejection. It is not yet clear whether allogeneic MSCs can induce a rejection response during bone formation. In this study, two strains of genetically unmatched mini-pigs were used as experimental animals to study the immunological changes in MSCs in vitro and in vivo when generating bone. Mini-pig MSCs showed low immunogenicity during osteogenesis both in vitro and in vivo, indicating that allogeneic MSCs had little or no immunogenicity in osteosis. In conclusion, allogeneic MSCs are an important source of seed cells for the tissue engineering of bone. This favours the clinical application of pre-constructed tissue-engineered bone.</description><subject>Animals</subject><subject>Bone and Bones - diagnostic imaging</subject><subject>Bone Marrow Cells - cytology</subject><subject>CD4-Positive T-Lymphocytes - cytology</subject><subject>CD8-Positive T-Lymphocytes - cytology</subject><subject>Cells, Cultured</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Flow Cytometry</subject><subject>Histocompatibility Antigens Class I - metabolism</subject><subject>Histocompatibility Antigens Class II - metabolism</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - immunology</subject><subject>Osteogenesis - immunology</subject><subject>Radiography</subject><subject>Swine</subject><subject>Swine, Miniature</subject><subject>Transplantation, Homologous</subject><issn>0300-0605</issn><issn>1473-2300</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UD1PwzAUtBCIlsIfYEDemEKf7XyOpaJQiY8BmCPHfimpErvYydB_j0sLCxLTSffuTveOkEsGN4xl2ZTFmeACAAoQGUDK2BEZ78hoxx6TMQSIIIVkRM68XwPEPE34KRlxYHGaZ2JMnpddNxjb2lWjZEtf-0Fvqa3prA0UGmwUfUKPRn1su-87dnSObeupHlxjVvTWGqQL6zrZN9ack5Nath4vDjgh74u7t_lD9Phyv5zPHiMlYt5HEvIakWlZCKZEIVPBU1lpSHilkyoXoCqtVMFz1DnUyAFVKCx1kimpAZiYkOt97sbZzwF9X3aNV6GXNGgHX2ZCpHkMRRGUfK9UznrvsC43rumk25YMyt2M5d8Zg-nqED9UHepfy89uQTDdC7xcYbm2gzPh3f8ivwAuNHqs</recordid><startdate>20091101</startdate><enddate>20091101</enddate><creator>Guo, SQ</creator><creator>Xu, JZ</creator><creator>Zou, QM</creator><creator>Jiang, DM</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20091101</creationdate><title>Immunological Study of Allogeneic Mesenchymal Stem Cells during Bone Formation</title><author>Guo, SQ ; Xu, JZ ; Zou, QM ; Jiang, DM</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-a08fee1da931c39a6326abd052bd5b830cbdcc928ed80fe20ec146ad57cad0013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Bone and Bones - diagnostic imaging</topic><topic>Bone Marrow Cells - cytology</topic><topic>CD4-Positive T-Lymphocytes - cytology</topic><topic>CD8-Positive T-Lymphocytes - cytology</topic><topic>Cells, Cultured</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Flow Cytometry</topic><topic>Histocompatibility Antigens Class I - metabolism</topic><topic>Histocompatibility Antigens Class II - metabolism</topic><topic>Lymphocyte Culture Test, Mixed</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - immunology</topic><topic>Osteogenesis - immunology</topic><topic>Radiography</topic><topic>Swine</topic><topic>Swine, Miniature</topic><topic>Transplantation, Homologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, SQ</creatorcontrib><creatorcontrib>Xu, JZ</creatorcontrib><creatorcontrib>Zou, QM</creatorcontrib><creatorcontrib>Jiang, DM</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of international medical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Guo, SQ</au><au>Xu, JZ</au><au>Zou, QM</au><au>Jiang, DM</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunological Study of Allogeneic Mesenchymal Stem Cells during Bone Formation</atitle><jtitle>Journal of international medical research</jtitle><addtitle>J Int Med Res</addtitle><date>2009-11-01</date><risdate>2009</risdate><volume>37</volume><issue>6</issue><spage>1750</spage><epage>1759</epage><pages>1750-1759</pages><issn>0300-0605</issn><eissn>1473-2300</eissn><abstract>Autologous mesenchymal stem cells (MSCs) are limited in their clinical application because tissue-engineered bone cannot be pre-fabricated. Allogeneic MSCs are readily available but carry the risk of transplant rejection. It is not yet clear whether allogeneic MSCs can induce a rejection response during bone formation. In this study, two strains of genetically unmatched mini-pigs were used as experimental animals to study the immunological changes in MSCs in vitro and in vivo when generating bone. Mini-pig MSCs showed low immunogenicity during osteogenesis both in vitro and in vivo, indicating that allogeneic MSCs had little or no immunogenicity in osteosis. In conclusion, allogeneic MSCs are an important source of seed cells for the tissue engineering of bone. This favours the clinical application of pre-constructed tissue-engineered bone.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>20146873</pmid><doi>10.1177/147323000903700611</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Bone and Bones - diagnostic imaging Bone Marrow Cells - cytology CD4-Positive T-Lymphocytes - cytology CD8-Positive T-Lymphocytes - cytology Cells, Cultured Enzyme-Linked Immunosorbent Assay Flow Cytometry Histocompatibility Antigens Class I - metabolism Histocompatibility Antigens Class II - metabolism Lymphocyte Culture Test, Mixed Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - immunology Osteogenesis - immunology Radiography Swine Swine, Miniature Transplantation, Homologous |
| title | Immunological Study of Allogeneic Mesenchymal Stem Cells during Bone Formation |
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