Expression of Somatostatin Receptors in Human Melanoma Cell Lines: Effect of Two Different Somatostatin Analogues, Octreotide and SOM230, on Cell Proliferation
Somatostatin analogues (SAs) are potential anticancer agents. This study was designed to investigate the expression of somatostatin receptors (SSTRs) in melanoma cells and the effect of two SAs on cell proliferation and viability. Eighteen primary and metastatic human cutaneous melanoma cell lines w...
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| Veröffentlicht in: | Journal of international medical research 2009-11, Vol.37 (6), p.1813-1822 |
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| creator | Martinez-Alonso, M Llecha, N Mayorga, ME Sorolla, A Dolcet, X Sanmartin, V Abal, L Casanova, JM Baradad, M Yeramian, A Egido, R Puig, S Vilella, R Matias-Guiu, X Marti, RM |
| description | Somatostatin analogues (SAs) are potential anticancer agents. This study was designed to investigate the expression of somatostatin receptors (SSTRs) in melanoma cells and the effect of two SAs on cell proliferation and viability. Eighteen primary and metastatic human cutaneous melanoma cell lines were treated with octreotide and SOM230. Expression of SSTR1, SSTR2, SSTR3 and SSTR5 was assessed by real-time polymerase chain reaction. Proliferation, viability and cell death were assessed using standard assays. Inhibition was modelled by mixed-effect regression. Melanoma cells expressed one or more SSTR. Both SAs inhibited proliferation of most melanoma cell lines, but inhibition was < 50%. Neither SA affected cell viability or induced cell death. The results suggest that melanoma cell lines express SSTRs. The SAs investigated, under the conditions used in this study, did not, however, significantly inhibit melanoma growth or induce cell death. Novel SAs, combination therapy with SAs and their anti-angiogenic properties should be further investigated. |
| doi_str_mv | 10.1177/147323000903700617 |
| format | Article |
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This study was designed to investigate the expression of somatostatin receptors (SSTRs) in melanoma cells and the effect of two SAs on cell proliferation and viability. Eighteen primary and metastatic human cutaneous melanoma cell lines were treated with octreotide and SOM230. Expression of SSTR1, SSTR2, SSTR3 and SSTR5 was assessed by real-time polymerase chain reaction. Proliferation, viability and cell death were assessed using standard assays. Inhibition was modelled by mixed-effect regression. Melanoma cells expressed one or more SSTR. Both SAs inhibited proliferation of most melanoma cell lines, but inhibition was < 50%. Neither SA affected cell viability or induced cell death. The results suggest that melanoma cell lines express SSTRs. The SAs investigated, under the conditions used in this study, did not, however, significantly inhibit melanoma growth or induce cell death. Novel SAs, combination therapy with SAs and their anti-angiogenic properties should be further investigated.</description><identifier>ISSN: 0300-0605</identifier><identifier>EISSN: 1473-2300</identifier><identifier>DOI: 10.1177/147323000903700617</identifier><identifier>PMID: 20146879</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Cell Line, Tumor ; Cell Proliferation - drug effects ; Gene Expression Regulation, Neoplastic - drug effects ; Humans ; Melanoma - genetics ; Melanoma - pathology ; Octreotide - pharmacology ; Receptors, Somatostatin - genetics ; Receptors, Somatostatin - metabolism ; Somatostatin - analogs & derivatives ; Somatostatin - pharmacology</subject><ispartof>Journal of international medical research, 2009-11, Vol.37 (6), p.1813-1822</ispartof><rights>2009 SAGE Publications</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-ce37fb76b6f4d03fa1f0cdb04babb70266ef5fa4a3df12e9afe3b475eb7b044b3</citedby><cites>FETCH-LOGICAL-c386t-ce37fb76b6f4d03fa1f0cdb04babb70266ef5fa4a3df12e9afe3b475eb7b044b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/147323000903700617$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/147323000903700617$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,777,781,21947,27834,27905,27906,44926,45314</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1177/147323000903700617?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20146879$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martinez-Alonso, M</creatorcontrib><creatorcontrib>Llecha, N</creatorcontrib><creatorcontrib>Mayorga, ME</creatorcontrib><creatorcontrib>Sorolla, A</creatorcontrib><creatorcontrib>Dolcet, X</creatorcontrib><creatorcontrib>Sanmartin, V</creatorcontrib><creatorcontrib>Abal, L</creatorcontrib><creatorcontrib>Casanova, JM</creatorcontrib><creatorcontrib>Baradad, M</creatorcontrib><creatorcontrib>Yeramian, A</creatorcontrib><creatorcontrib>Egido, R</creatorcontrib><creatorcontrib>Puig, S</creatorcontrib><creatorcontrib>Vilella, R</creatorcontrib><creatorcontrib>Matias-Guiu, X</creatorcontrib><creatorcontrib>Marti, RM</creatorcontrib><title>Expression of Somatostatin Receptors in Human Melanoma Cell Lines: Effect of Two Different Somatostatin Analogues, Octreotide and SOM230, on Cell Proliferation</title><title>Journal of international medical research</title><addtitle>J Int Med Res</addtitle><description>Somatostatin analogues (SAs) are potential anticancer agents. This study was designed to investigate the expression of somatostatin receptors (SSTRs) in melanoma cells and the effect of two SAs on cell proliferation and viability. Eighteen primary and metastatic human cutaneous melanoma cell lines were treated with octreotide and SOM230. Expression of SSTR1, SSTR2, SSTR3 and SSTR5 was assessed by real-time polymerase chain reaction. Proliferation, viability and cell death were assessed using standard assays. Inhibition was modelled by mixed-effect regression. Melanoma cells expressed one or more SSTR. Both SAs inhibited proliferation of most melanoma cell lines, but inhibition was < 50%. Neither SA affected cell viability or induced cell death. The results suggest that melanoma cell lines express SSTRs. The SAs investigated, under the conditions used in this study, did not, however, significantly inhibit melanoma growth or induce cell death. Novel SAs, combination therapy with SAs and their anti-angiogenic properties should be further investigated.</description><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Humans</subject><subject>Melanoma - genetics</subject><subject>Melanoma - pathology</subject><subject>Octreotide - pharmacology</subject><subject>Receptors, Somatostatin - genetics</subject><subject>Receptors, Somatostatin - metabolism</subject><subject>Somatostatin - analogs & derivatives</subject><subject>Somatostatin - pharmacology</subject><issn>0300-0605</issn><issn>1473-2300</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctKNDEQhYP8ouPlBVxIdv_G1kqnJ5lxJ-N4gZERL-sm6a5IS3cyJmnUp_FVzTgqiOCqqOI7h0MdQvYYHDIm5RErJM85AIyBSwDB5BoZLI_Z8vqPDCCNDAQMN8lWCI8ARS6G-QbZzIEVYiTHA_I2fVl4DKFxljpDb12nogtRxcbSG6xwEZ0PNC0XfacsvcJW2cTQCbYtnTUWwzGdGoNVXMrvnh09bdLq0cafZidWte6hx3BA51X06GJTI1W2prfzq5T3gKYEH67X3rVNskgyZ3fIulFtwN3PuU3uz6Z3k4tsNj-_nJzMsoqPRMwq5NJoKbQwRQ3cKGagqjUUWmktIRcCzdCoQvHasBzHyiDXhRyilgkqNN8m_1e-C--eUspYdk2oUhxl0fWhlJyLER9Bnsh8RVbeheDRlAvfdMq_lgzKZS_l716SaP_Tvtcd1t-SryIScLQCgnrA8tH1Pv0r_GX5DpFjmAw</recordid><startdate>20091101</startdate><enddate>20091101</enddate><creator>Martinez-Alonso, M</creator><creator>Llecha, N</creator><creator>Mayorga, ME</creator><creator>Sorolla, A</creator><creator>Dolcet, X</creator><creator>Sanmartin, V</creator><creator>Abal, L</creator><creator>Casanova, JM</creator><creator>Baradad, M</creator><creator>Yeramian, A</creator><creator>Egido, R</creator><creator>Puig, S</creator><creator>Vilella, R</creator><creator>Matias-Guiu, X</creator><creator>Marti, RM</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20091101</creationdate><title>Expression of Somatostatin Receptors in Human Melanoma Cell Lines: Effect of Two Different Somatostatin Analogues, Octreotide and SOM230, on Cell Proliferation</title><author>Martinez-Alonso, M ; Llecha, N ; Mayorga, ME ; Sorolla, A ; Dolcet, X ; Sanmartin, V ; Abal, L ; Casanova, JM ; Baradad, M ; Yeramian, A ; Egido, R ; Puig, S ; Vilella, R ; Matias-Guiu, X ; Marti, RM</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-ce37fb76b6f4d03fa1f0cdb04babb70266ef5fa4a3df12e9afe3b475eb7b044b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Humans</topic><topic>Melanoma - genetics</topic><topic>Melanoma - pathology</topic><topic>Octreotide - pharmacology</topic><topic>Receptors, Somatostatin - genetics</topic><topic>Receptors, Somatostatin - metabolism</topic><topic>Somatostatin - analogs & derivatives</topic><topic>Somatostatin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martinez-Alonso, M</creatorcontrib><creatorcontrib>Llecha, N</creatorcontrib><creatorcontrib>Mayorga, ME</creatorcontrib><creatorcontrib>Sorolla, A</creatorcontrib><creatorcontrib>Dolcet, X</creatorcontrib><creatorcontrib>Sanmartin, V</creatorcontrib><creatorcontrib>Abal, L</creatorcontrib><creatorcontrib>Casanova, JM</creatorcontrib><creatorcontrib>Baradad, M</creatorcontrib><creatorcontrib>Yeramian, A</creatorcontrib><creatorcontrib>Egido, R</creatorcontrib><creatorcontrib>Puig, S</creatorcontrib><creatorcontrib>Vilella, R</creatorcontrib><creatorcontrib>Matias-Guiu, X</creatorcontrib><creatorcontrib>Marti, RM</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of international medical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Martinez-Alonso, M</au><au>Llecha, N</au><au>Mayorga, ME</au><au>Sorolla, A</au><au>Dolcet, X</au><au>Sanmartin, V</au><au>Abal, L</au><au>Casanova, JM</au><au>Baradad, M</au><au>Yeramian, A</au><au>Egido, R</au><au>Puig, S</au><au>Vilella, R</au><au>Matias-Guiu, X</au><au>Marti, RM</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Somatostatin Receptors in Human Melanoma Cell Lines: Effect of Two Different Somatostatin Analogues, Octreotide and SOM230, on Cell Proliferation</atitle><jtitle>Journal of international medical research</jtitle><addtitle>J Int Med Res</addtitle><date>2009-11-01</date><risdate>2009</risdate><volume>37</volume><issue>6</issue><spage>1813</spage><epage>1822</epage><pages>1813-1822</pages><issn>0300-0605</issn><eissn>1473-2300</eissn><abstract>Somatostatin analogues (SAs) are potential anticancer agents. This study was designed to investigate the expression of somatostatin receptors (SSTRs) in melanoma cells and the effect of two SAs on cell proliferation and viability. Eighteen primary and metastatic human cutaneous melanoma cell lines were treated with octreotide and SOM230. Expression of SSTR1, SSTR2, SSTR3 and SSTR5 was assessed by real-time polymerase chain reaction. Proliferation, viability and cell death were assessed using standard assays. Inhibition was modelled by mixed-effect regression. Melanoma cells expressed one or more SSTR. Both SAs inhibited proliferation of most melanoma cell lines, but inhibition was < 50%. Neither SA affected cell viability or induced cell death. The results suggest that melanoma cell lines express SSTRs. The SAs investigated, under the conditions used in this study, did not, however, significantly inhibit melanoma growth or induce cell death. Novel SAs, combination therapy with SAs and their anti-angiogenic properties should be further investigated.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>20146879</pmid><doi>10.1177/147323000903700617</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0300-0605 |
| ispartof | Journal of international medical research, 2009-11, Vol.37 (6), p.1813-1822 |
| issn | 0300-0605 1473-2300 |
| language | eng |
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| source | Sage Journals GOLD Open Access 2024 |
| subjects | Cell Line, Tumor Cell Proliferation - drug effects Gene Expression Regulation, Neoplastic - drug effects Humans Melanoma - genetics Melanoma - pathology Octreotide - pharmacology Receptors, Somatostatin - genetics Receptors, Somatostatin - metabolism Somatostatin - analogs & derivatives Somatostatin - pharmacology |
| title | Expression of Somatostatin Receptors in Human Melanoma Cell Lines: Effect of Two Different Somatostatin Analogues, Octreotide and SOM230, on Cell Proliferation |
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