Cloning, expression, and characterization of a dithiol glutaredoxin from Trypanosoma cruzi

Glutaredoxins play an important role in cellular functionality. A putative dithiol glutaredoxin is encoded in the genome of Trypanosoma cruzi. We cloned the gene and obtained the recombinant protein, which behaved as a typical thioltransferase. Activity was variable and dependent on the nature of re...

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Veröffentlicht in:	Antioxidants & redox signaling 2010-03, Vol.12 (6), p.787-792
Hauptverfasser:	Marquez, Vanina E, Arias, Diego G, Piattoni, Claudia V, Robello, Carlos, Iglesias, Alberto A, Guerrero, Sergio A
Format:	Artikel
Sprache:	eng
Schlagworte:	Active oxygen Amino Acid Sequence Cellular proteins Cloning, Molecular Genetic aspects Glutaredoxins - genetics Glutaredoxins - metabolism Health aspects Molecular Sequence Data Oxidation-Reduction Physiological aspects Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Alignment Trypanosoma cruzi Trypanosoma cruzi - enzymology Trypanosoma cruzi - genetics
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