In Vivo Evaluation of Hydroxyapatite Nanocoating on Polyester Artificial Vascular Grafts and Possibility as Soft-Tissue Compatible Material
The efficacy of hydroxyapatite (HAp) nanocoating on polyester vascular grafts was investigated in animal experiments. The HAp nanocrystals were covalently bonded separately between hydroxyl groups on a nanocrystal and alkoxysilyl groups in γ-methacryloxypropyl triethoxysilane graft polymerized on a...
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Veröffentlicht in: | ASAIO journal (1992) 2010-01, Vol.56 (1), p.61-66 |
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creator | Kadono, Hiroyuki Furuzono, Tsutomu Masuda, Miwa Okada, Masahiro Ueki, Mitsuki Takamizawa, Keiichi Tanaka, Ryoichi Miyatake, Kunio Koyama, Yoshihisa Takakuda, Kazuo |
description | The efficacy of hydroxyapatite (HAp) nanocoating on polyester vascular grafts was investigated in animal experiments. The HAp nanocrystals were covalently bonded separately between hydroxyl groups on a nanocrystal and alkoxysilyl groups in γ-methacryloxypropyl triethoxysilane graft polymerized on a polyester substrate. Twelve HAp-coated polyester grafts and 10 control grafts of 20, 30, or 50 mm in length were implanted in canine common carotid arteries. Serious complications or occlusions were not observed in any of the dogs after implantation. A histologic evaluation was conducted by staining with hematoxylin and eosin (HE), the von Willebrand factor (vWf), and α-smooth muscle actin (α-SMA) around the inner lumen of the grafts. The number of inflammation cells and giant cells in the HAp-coated group was significantly lower than that in the group receiving noncoated grafts (p < 0.05). |
doi_str_mv | 10.1097/MAT.0b013e3181c945ae |
format | Article |
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The HAp nanocrystals were covalently bonded separately between hydroxyl groups on a nanocrystal and alkoxysilyl groups in γ-methacryloxypropyl triethoxysilane graft polymerized on a polyester substrate. Twelve HAp-coated polyester grafts and 10 control grafts of 20, 30, or 50 mm in length were implanted in canine common carotid arteries. Serious complications or occlusions were not observed in any of the dogs after implantation. A histologic evaluation was conducted by staining with hematoxylin and eosin (HE), the von Willebrand factor (vWf), and α-smooth muscle actin (α-SMA) around the inner lumen of the grafts. 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The HAp nanocrystals were covalently bonded separately between hydroxyl groups on a nanocrystal and alkoxysilyl groups in γ-methacryloxypropyl triethoxysilane graft polymerized on a polyester substrate. Twelve HAp-coated polyester grafts and 10 control grafts of 20, 30, or 50 mm in length were implanted in canine common carotid arteries. Serious complications or occlusions were not observed in any of the dogs after implantation. A histologic evaluation was conducted by staining with hematoxylin and eosin (HE), the von Willebrand factor (vWf), and α-smooth muscle actin (α-SMA) around the inner lumen of the grafts. The number of inflammation cells and giant cells in the HAp-coated group was significantly lower than that in the group receiving noncoated grafts (p < 0.05).</description><subject>Animals</subject><subject>Blood Vessel Prosthesis - adverse effects</subject><subject>Carotid Arteries</subject><subject>Coated Materials, Biocompatible - adverse effects</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Compliance</subject><subject>Dogs</subject><subject>Durapatite - chemistry</subject><subject>Inflammation - etiology</subject><subject>Inflammation - pathology</subject><subject>Materials Testing</subject><subject>Microscopy, Electron, Scanning</subject><subject>Polyesters</subject><issn>1058-2916</issn><issn>1538-943X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtP3DAUha2qVaHQf1Ah77oKteM8nOVoxEviJTGg7qIb57qYeuKpnUDzG_qne9EAiy668pX93WOdcxj7IsWhFE397WKxOhSdkAqV1NI0RQn4ju3KUumsKdT39zSLUmd5I6sd9imlByHoUcmPbCcXopRayV3252zgd-4x8KNH8BOMLgw8WH469zH8nmFDNyPySxiCCTQPPzgB18HPmEaMfBFHZ51x4PkdJDN5iPwkgh0Th6EnMCXXOe_GmUPiN8GO2cqlNCFfhvWzeOeRXwBJkcQ--2DBJ_z8cu6x2-Oj1fI0O786OVsuzjNTVDVmDVa9QtOZXhsJqrfYi6awlYQytwJUbXMhtVCYl11RGihFZykfqxXFVStQe-zrVncTw6-JjLRrlwx6DwOGKbW1UpXOi0YQWWxJE8lJRNtuoltDnFsp2ucWWmqh_bcFWjt4-WDq1ti_Lb3GToDeAk_Bk_f0009PGNt7BD_e_1_7Ly0umgM</recordid><startdate>201001</startdate><enddate>201001</enddate><creator>Kadono, Hiroyuki</creator><creator>Furuzono, Tsutomu</creator><creator>Masuda, Miwa</creator><creator>Okada, Masahiro</creator><creator>Ueki, Mitsuki</creator><creator>Takamizawa, Keiichi</creator><creator>Tanaka, Ryoichi</creator><creator>Miyatake, Kunio</creator><creator>Koyama, Yoshihisa</creator><creator>Takakuda, Kazuo</creator><general>Amercian Society of Artificial Internal Organs</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201001</creationdate><title>In Vivo Evaluation of Hydroxyapatite Nanocoating on Polyester Artificial Vascular Grafts and Possibility as Soft-Tissue Compatible Material</title><author>Kadono, Hiroyuki ; 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subjects | Animals Blood Vessel Prosthesis - adverse effects Carotid Arteries Coated Materials, Biocompatible - adverse effects Coated Materials, Biocompatible - chemistry Compliance Dogs Durapatite - chemistry Inflammation - etiology Inflammation - pathology Materials Testing Microscopy, Electron, Scanning Polyesters |
title | In Vivo Evaluation of Hydroxyapatite Nanocoating on Polyester Artificial Vascular Grafts and Possibility as Soft-Tissue Compatible Material |
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