Formation of primordial follicles and immunolocalization of PTEN, PKB and FOXO3A proteins in the ovaries of fetal and neonatal pigs
The assembly of primordial follicles and subsequent development and transition of the primordial follicle to the primary follicle are critical processes in ovarian biology. In order to examine follicle formation and development in fetal and neonatal pigs, ovarian samples were obtained from a famous...
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description | The assembly of primordial follicles and subsequent development and transition of the primordial follicle to the primary follicle are critical processes in ovarian biology. In order to examine follicle formation and development in fetal and neonatal pigs, ovarian samples were obtained from a famous local breed of Chinese pigs, Erhualian pigs, ranging in age from 50 days postcoitum to 1 day postpartum in our current study. Morphological changes in the ovaries of the fetal and neonatal pigs indicated that egg nests were the earliest recognizable gamete cells. The proportion of egg nests decreased from 98.4 to 25.6% and the proportion of single follicles increased from 1.6 to 74.4% between 70 and 90 days postcoitum. The proportions of primordial follicles increased between 70 and 90 days postcoitum but decreased from 90 days postcoitum to 1 day postpartum. Our results suggested that the key stage of primordial follicle formation was between 70 and 90 days postcoitum and that the major stage of transition from primordial follicles into primary follicles was between 90 days postcoitum and 1 day postpartum. Experiments were also conducted to examine the localization of PTEN, PKB and FOXO3A proteins in the porcine ovaries by immunohistochemistry and immunoblotting. The results indicated that PTEN, PKB and FOXO3A were localized in the germ cells of egg nests, cytoplasm of oocytes and granulosa cells of follicles ranging from the primordial to secondary stages and that the staining intensity was weak in granulosa cells but strong in oocytes. The different staining patterns of PTEN, FOXO3A and PKB suggested that these proteins were expressed in a stage- and cell-specific manner during ovarian follicle formation and development in the fetal and neonatal pig. |
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(China) ; Wang, W ; Zhou, B ; Zhang, W ; Huang, P ; Shi, F ; Taya, K</creator><creatorcontrib>Ding, W., Nanjing Agricultural Univ. (China) ; Wang, W ; Zhou, B ; Zhang, W ; Huang, P ; Shi, F ; Taya, K</creatorcontrib><description>The assembly of primordial follicles and subsequent development and transition of the primordial follicle to the primary follicle are critical processes in ovarian biology. In order to examine follicle formation and development in fetal and neonatal pigs, ovarian samples were obtained from a famous local breed of Chinese pigs, Erhualian pigs, ranging in age from 50 days postcoitum to 1 day postpartum in our current study. Morphological changes in the ovaries of the fetal and neonatal pigs indicated that egg nests were the earliest recognizable gamete cells. The proportion of egg nests decreased from 98.4 to 25.6% and the proportion of single follicles increased from 1.6 to 74.4% between 70 and 90 days postcoitum. The proportions of primordial follicles increased between 70 and 90 days postcoitum but decreased from 90 days postcoitum to 1 day postpartum. Our results suggested that the key stage of primordial follicle formation was between 70 and 90 days postcoitum and that the major stage of transition from primordial follicles into primary follicles was between 90 days postcoitum and 1 day postpartum. Experiments were also conducted to examine the localization of PTEN, PKB and FOXO3A proteins in the porcine ovaries by immunohistochemistry and immunoblotting. The results indicated that PTEN, PKB and FOXO3A were localized in the germ cells of egg nests, cytoplasm of oocytes and granulosa cells of follicles ranging from the primordial to secondary stages and that the staining intensity was weak in granulosa cells but strong in oocytes. The different staining patterns of PTEN, FOXO3A and PKB suggested that these proteins were expressed in a stage- and cell-specific manner during ovarian follicle formation and development in the fetal and neonatal pig.</description><identifier>ISSN: 0916-8818</identifier><identifier>EISSN: 1348-4400</identifier><identifier>DOI: 10.1262/jrd.09-094h</identifier><identifier>PMID: 19996554</identifier><language>eng</language><publisher>Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</publisher><subject>3-Phosphoinositide-Dependent Protein Kinases ; ANIMAL NOUVEAU-NE ; ANIMAL RECIEN NACIDO ; Animals ; CERDO ; Female ; FETO ; Fetus - metabolism ; FOETUS ; FOLICULOS OVARICOS ; FOLLICULE OVARIEN ; Forkhead Transcription Factors - immunology ; Forkhead Transcription Factors - metabolism ; FOXO3A ; Granulosa Cells - cytology ; Granulosa Cells - metabolism ; http://www.fao.org/aos/agrovoc#c_12815 ; http://www.fao.org/aos/agrovoc#c_3006 ; http://www.fao.org/aos/agrovoc#c_32465 ; http://www.fao.org/aos/agrovoc#c_5456 ; http://www.fao.org/aos/agrovoc#c_6259 ; http://www.fao.org/aos/agrovoc#c_7555 ; Immunohistochemistry ; NEWBORN ANIMALS ; OVAIRE ; Ovarian Follicle - cytology ; Ovarian Follicle - growth & development ; Ovarian Follicle - metabolism ; OVARIAN FOLLICLES ; OVARIES ; OVARIOS ; Ovary ; Ovary - cytology ; Ovary - growth & development ; Ovary - metabolism ; Pig ; PORCIN ; Primordial follicle ; Protein-Serine-Threonine Kinases - immunology ; Protein-Serine-Threonine Kinases - metabolism ; PROTEINAS ; PROTEINE ; PROTEINS ; PTEN ; PTEN Phosphohydrolase - immunology ; PTEN Phosphohydrolase - metabolism ; SWINE ; Swine - growth & development ; Swine - metabolism</subject><ispartof>Journal of Reproduction and Development, 2010, Vol.56(1), pp.162-168</ispartof><rights>2010 Society for Reproduction and Development</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c578t-631d1682638bd098b0cfc17abf92a2adb9698859fc1830f1baeda9c12cc629643</citedby><cites>FETCH-LOGICAL-c578t-631d1682638bd098b0cfc17abf92a2adb9698859fc1830f1baeda9c12cc629643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,1881,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19996554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ding, W., Nanjing Agricultural Univ. (China)</creatorcontrib><creatorcontrib>Wang, W</creatorcontrib><creatorcontrib>Zhou, B</creatorcontrib><creatorcontrib>Zhang, W</creatorcontrib><creatorcontrib>Huang, P</creatorcontrib><creatorcontrib>Shi, F</creatorcontrib><creatorcontrib>Taya, K</creatorcontrib><title>Formation of primordial follicles and immunolocalization of PTEN, PKB and FOXO3A proteins in the ovaries of fetal and neonatal pigs</title><title>Journal of Reproduction and Development</title><addtitle>J. Reprod. Dev.</addtitle><description>The assembly of primordial follicles and subsequent development and transition of the primordial follicle to the primary follicle are critical processes in ovarian biology. In order to examine follicle formation and development in fetal and neonatal pigs, ovarian samples were obtained from a famous local breed of Chinese pigs, Erhualian pigs, ranging in age from 50 days postcoitum to 1 day postpartum in our current study. Morphological changes in the ovaries of the fetal and neonatal pigs indicated that egg nests were the earliest recognizable gamete cells. The proportion of egg nests decreased from 98.4 to 25.6% and the proportion of single follicles increased from 1.6 to 74.4% between 70 and 90 days postcoitum. The proportions of primordial follicles increased between 70 and 90 days postcoitum but decreased from 90 days postcoitum to 1 day postpartum. Our results suggested that the key stage of primordial follicle formation was between 70 and 90 days postcoitum and that the major stage of transition from primordial follicles into primary follicles was between 90 days postcoitum and 1 day postpartum. Experiments were also conducted to examine the localization of PTEN, PKB and FOXO3A proteins in the porcine ovaries by immunohistochemistry and immunoblotting. The results indicated that PTEN, PKB and FOXO3A were localized in the germ cells of egg nests, cytoplasm of oocytes and granulosa cells of follicles ranging from the primordial to secondary stages and that the staining intensity was weak in granulosa cells but strong in oocytes. The different staining patterns of PTEN, FOXO3A and PKB suggested that these proteins were expressed in a stage- and cell-specific manner during ovarian follicle formation and development in the fetal and neonatal pig.</description><subject>3-Phosphoinositide-Dependent Protein Kinases</subject><subject>ANIMAL NOUVEAU-NE</subject><subject>ANIMAL RECIEN NACIDO</subject><subject>Animals</subject><subject>CERDO</subject><subject>Female</subject><subject>FETO</subject><subject>Fetus - metabolism</subject><subject>FOETUS</subject><subject>FOLICULOS OVARICOS</subject><subject>FOLLICULE OVARIEN</subject><subject>Forkhead Transcription Factors - immunology</subject><subject>Forkhead Transcription Factors - metabolism</subject><subject>FOXO3A</subject><subject>Granulosa Cells - cytology</subject><subject>Granulosa Cells - metabolism</subject><subject>http://www.fao.org/aos/agrovoc#c_12815</subject><subject>http://www.fao.org/aos/agrovoc#c_3006</subject><subject>http://www.fao.org/aos/agrovoc#c_32465</subject><subject>http://www.fao.org/aos/agrovoc#c_5456</subject><subject>http://www.fao.org/aos/agrovoc#c_6259</subject><subject>http://www.fao.org/aos/agrovoc#c_7555</subject><subject>Immunohistochemistry</subject><subject>NEWBORN ANIMALS</subject><subject>OVAIRE</subject><subject>Ovarian Follicle - cytology</subject><subject>Ovarian Follicle - growth & development</subject><subject>Ovarian Follicle - metabolism</subject><subject>OVARIAN FOLLICLES</subject><subject>OVARIES</subject><subject>OVARIOS</subject><subject>Ovary</subject><subject>Ovary - cytology</subject><subject>Ovary - growth & development</subject><subject>Ovary - metabolism</subject><subject>Pig</subject><subject>PORCIN</subject><subject>Primordial follicle</subject><subject>Protein-Serine-Threonine Kinases - immunology</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>PROTEINS</subject><subject>PTEN</subject><subject>PTEN Phosphohydrolase - immunology</subject><subject>PTEN Phosphohydrolase - metabolism</subject><subject>SWINE</subject><subject>Swine - growth & development</subject><subject>Swine - metabolism</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9v0zAYQC0EYt3gxBmUG4ctwz8Sxz6WaWVj09rDkLhZXxy7deXEm50iwZV_HGetimTZsv309Okh9IHgS0I5_bKN3SWWJZbV5hWaEVaJsqowfo1mWBJeCkHECTpNaYsxozWv3qITIqXkdV3N0N9FiD2MLgxFsMVTdH2InQNf2OC9096kAoaucH2_G4IPGrz7c8RXj9cPF8Xq7usLs1j-XLJ5doTRuCEVbijGjSnCL4guazJvzZjNEzuYMMB0eXLr9A69seCTeX84z9CPxfXj1U15v_x2ezW_L3XdiLHkjHSEC8qZaDssRYu11aSB1koKFLpWcilELfOjYNiSFkwHUhOqNaeSV-wMfd5784jPO5NG1bukjfeQx9kl1TDGm7rmNJPne1LHkFI0Vk1lIP5WBKspusrRFZZ5VTeZ_nTw7tredP_ZQ-UMzPfANo2wNkcA4jglfpHVXJFpO0iPf3oDUZkhOz7uHRaCgnV0SX1fUUwwxpQ2gv0DUpieZw</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Ding, W., Nanjing Agricultural Univ. 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(China)</creatorcontrib><creatorcontrib>Wang, W</creatorcontrib><creatorcontrib>Zhou, B</creatorcontrib><creatorcontrib>Zhang, W</creatorcontrib><creatorcontrib>Huang, P</creatorcontrib><creatorcontrib>Shi, F</creatorcontrib><creatorcontrib>Taya, K</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Reproduction and Development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ding, W., Nanjing Agricultural Univ. (China)</au><au>Wang, W</au><au>Zhou, B</au><au>Zhang, W</au><au>Huang, P</au><au>Shi, F</au><au>Taya, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Formation of primordial follicles and immunolocalization of PTEN, PKB and FOXO3A proteins in the ovaries of fetal and neonatal pigs</atitle><jtitle>Journal of Reproduction and Development</jtitle><addtitle>J. Reprod. Dev.</addtitle><date>2010-02-01</date><risdate>2010</risdate><volume>56</volume><issue>1</issue><spage>162</spage><epage>168</epage><pages>162-168</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract>The assembly of primordial follicles and subsequent development and transition of the primordial follicle to the primary follicle are critical processes in ovarian biology. In order to examine follicle formation and development in fetal and neonatal pigs, ovarian samples were obtained from a famous local breed of Chinese pigs, Erhualian pigs, ranging in age from 50 days postcoitum to 1 day postpartum in our current study. Morphological changes in the ovaries of the fetal and neonatal pigs indicated that egg nests were the earliest recognizable gamete cells. The proportion of egg nests decreased from 98.4 to 25.6% and the proportion of single follicles increased from 1.6 to 74.4% between 70 and 90 days postcoitum. The proportions of primordial follicles increased between 70 and 90 days postcoitum but decreased from 90 days postcoitum to 1 day postpartum. Our results suggested that the key stage of primordial follicle formation was between 70 and 90 days postcoitum and that the major stage of transition from primordial follicles into primary follicles was between 90 days postcoitum and 1 day postpartum. Experiments were also conducted to examine the localization of PTEN, PKB and FOXO3A proteins in the porcine ovaries by immunohistochemistry and immunoblotting. The results indicated that PTEN, PKB and FOXO3A were localized in the germ cells of egg nests, cytoplasm of oocytes and granulosa cells of follicles ranging from the primordial to secondary stages and that the staining intensity was weak in granulosa cells but strong in oocytes. The different staining patterns of PTEN, FOXO3A and PKB suggested that these proteins were expressed in a stage- and cell-specific manner during ovarian follicle formation and development in the fetal and neonatal pig.</abstract><cop>Japan</cop><pub>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</pub><pmid>19996554</pmid><doi>10.1262/jrd.09-094h</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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source | J-STAGE Free; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
subjects | 3-Phosphoinositide-Dependent Protein Kinases ANIMAL NOUVEAU-NE ANIMAL RECIEN NACIDO Animals CERDO Female FETO Fetus - metabolism FOETUS FOLICULOS OVARICOS FOLLICULE OVARIEN Forkhead Transcription Factors - immunology Forkhead Transcription Factors - metabolism FOXO3A Granulosa Cells - cytology Granulosa Cells - metabolism http://www.fao.org/aos/agrovoc#c_12815 http://www.fao.org/aos/agrovoc#c_3006 http://www.fao.org/aos/agrovoc#c_32465 http://www.fao.org/aos/agrovoc#c_5456 http://www.fao.org/aos/agrovoc#c_6259 http://www.fao.org/aos/agrovoc#c_7555 Immunohistochemistry NEWBORN ANIMALS OVAIRE Ovarian Follicle - cytology Ovarian Follicle - growth & development Ovarian Follicle - metabolism OVARIAN FOLLICLES OVARIES OVARIOS Ovary Ovary - cytology Ovary - growth & development Ovary - metabolism Pig PORCIN Primordial follicle Protein-Serine-Threonine Kinases - immunology Protein-Serine-Threonine Kinases - metabolism PROTEINAS PROTEINE PROTEINS PTEN PTEN Phosphohydrolase - immunology PTEN Phosphohydrolase - metabolism SWINE Swine - growth & development Swine - metabolism |
title | Formation of primordial follicles and immunolocalization of PTEN, PKB and FOXO3A proteins in the ovaries of fetal and neonatal pigs |
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