The critical role of ocular-infiltrating macrophages in the development of choroidal neovascularization
Choroidal neovascularization (CNV) is directly related to visual loss in some eye diseases, such as age‐related macular degeneration. Although several human histological studies have suggested the participation of macrophages in CNV formation, the precise mechanisms are still not fully understood. I...
Gespeichert in:
| Veröffentlicht in: | Journal of leukocyte biology 2003-07, Vol.74 (1), p.25-32 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 32 |
|---|---|
| container_issue | 1 |
| container_start_page | 25 |
| container_title | Journal of leukocyte biology |
| container_volume | 74 |
| creator | Tsutsumi, Chikako Sonoda, Koh‐Hei Egashira, Kensuke Qiao, Hong Hisatomi, Toshio Nakao, Shintaro Ishibashi, Minako Charo, Israel F. Sakamoto, Taiji Murata, Toshinori Ishibashi, Tatsuro |
| description | Choroidal neovascularization (CNV) is directly related to visual loss in some eye diseases, such as age‐related macular degeneration. Although several human histological studies have suggested the participation of macrophages in CNV formation, the precise mechanisms are still not fully understood. In this study, we elucidated the role of ocular‐infiltrating macrophages in experimental CNV using CCR2 knockout (KO) mice, wild‐type mice, and C57BL/6 (B6) mice. CCR2 is the receptor of monocyte chemoattractant protein‐1, and the number of infiltrating macrophage and the area of CNV were significantly reduced in CCR2 KO mice. Enriched ocular‐infiltrating macrophages from B6 mice actually showed angiogenic ability in a dorsal air sac assay. Moreover, their expression of class II, CD40, B7‐1 and B7‐2 molecules, and the mRNA for potential angiogenic factors, such as vascular endothelial growth factor, basic fibroblast growth factor, and tumor necrosis factor α, was also observed. Collectively, we conclude that ocular‐infiltrating macrophages play an important role in CNV generation. |
| doi_str_mv | 10.1189/jlb.0902436 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73367001</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73367001</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4895-bf27ebacdbd6aa556d64c1409493c9765c26b7e960e61f829a35f0d615692efa3</originalsourceid><addsrcrecordid>eNqFkUFP3DAQRq2qqGyhp96rHKpeqsDYjp34SBG0VCtxgbPlOJONkRNv7SwR_PoadqXe6MmX970Zz0fIZwpnlDbq_MG3Z6CAVVy-IyuqeFNyWfP3ZAV1RUtRARyTjyk9AABnEj6QY8oannm1Ipu7AQsb3eys8UUMHovQF8HuvImlm3rn52hmN22K0dgYtoPZYCrcVMw51-Ej-rAdcZpfUnYIMbgueyYMjya9StxzjofplBz1xif8dHhPyP311d3lr3J9-_Pm8mJd2qpRomx7VmNrbNd20hghZCcrSytQleJW1VJYJtsalQSUtG-YMlz00EkqpGLYG35Cvu292xj-7DDNenTJovcm77RLuub5NgD0vyBVjAspeQa_78H8_ZQi9nob3Wjik6agXwrQuQB9KCDTXw7aXTti9489XDwDsAcW5_HpLZf-vf4BwESOfN1HBrcZFhdRp9F4nycwvSxLXWmqM_YXfaeeaA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19235663</pqid></control><display><type>article</type><title>The critical role of ocular-infiltrating macrophages in the development of choroidal neovascularization</title><source>MEDLINE</source><source>Wiley Online Library</source><source>Oxford Academic Journals (OUP)</source><source>EZB Electronic Journals Library</source><creator>Tsutsumi, Chikako ; Sonoda, Koh‐Hei ; Egashira, Kensuke ; Qiao, Hong ; Hisatomi, Toshio ; Nakao, Shintaro ; Ishibashi, Minako ; Charo, Israel F. ; Sakamoto, Taiji ; Murata, Toshinori ; Ishibashi, Tatsuro</creator><creatorcontrib>Tsutsumi, Chikako ; Sonoda, Koh‐Hei ; Egashira, Kensuke ; Qiao, Hong ; Hisatomi, Toshio ; Nakao, Shintaro ; Ishibashi, Minako ; Charo, Israel F. ; Sakamoto, Taiji ; Murata, Toshinori ; Ishibashi, Tatsuro</creatorcontrib><description>Choroidal neovascularization (CNV) is directly related to visual loss in some eye diseases, such as age‐related macular degeneration. Although several human histological studies have suggested the participation of macrophages in CNV formation, the precise mechanisms are still not fully understood. In this study, we elucidated the role of ocular‐infiltrating macrophages in experimental CNV using CCR2 knockout (KO) mice, wild‐type mice, and C57BL/6 (B6) mice. CCR2 is the receptor of monocyte chemoattractant protein‐1, and the number of infiltrating macrophage and the area of CNV were significantly reduced in CCR2 KO mice. Enriched ocular‐infiltrating macrophages from B6 mice actually showed angiogenic ability in a dorsal air sac assay. Moreover, their expression of class II, CD40, B7‐1 and B7‐2 molecules, and the mRNA for potential angiogenic factors, such as vascular endothelial growth factor, basic fibroblast growth factor, and tumor necrosis factor α, was also observed. Collectively, we conclude that ocular‐infiltrating macrophages play an important role in CNV generation.</description><identifier>ISSN: 0741-5400</identifier><identifier>EISSN: 1938-3673</identifier><identifier>DOI: 10.1189/jlb.0902436</identifier><identifier>PMID: 12832439</identifier><language>eng</language><publisher>United States: Society for Leukocyte Biology</publisher><subject>age-related macular degeneration ; Animals ; Cell Movement - immunology ; Choroidal Neovascularization ; Eye - blood supply ; Eye - pathology ; Growth Substances - biosynthesis ; Growth Substances - genetics ; Histocompatibility Antigens Class II - biosynthesis ; Macrophages - physiology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; photocoagulation ; Receptors, CCR2 ; Receptors, Chemokine ; retinal pigment epithelium ; RNA, Messenger - analysis</subject><ispartof>Journal of leukocyte biology, 2003-07, Vol.74 (1), p.25-32</ispartof><rights>2003 Society for Leukocyte Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4895-bf27ebacdbd6aa556d64c1409493c9765c26b7e960e61f829a35f0d615692efa3</citedby><cites>FETCH-LOGICAL-c4895-bf27ebacdbd6aa556d64c1409493c9765c26b7e960e61f829a35f0d615692efa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1189%2Fjlb.0902436$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1189%2Fjlb.0902436$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27926,27927,45576,45577</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12832439$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsutsumi, Chikako</creatorcontrib><creatorcontrib>Sonoda, Koh‐Hei</creatorcontrib><creatorcontrib>Egashira, Kensuke</creatorcontrib><creatorcontrib>Qiao, Hong</creatorcontrib><creatorcontrib>Hisatomi, Toshio</creatorcontrib><creatorcontrib>Nakao, Shintaro</creatorcontrib><creatorcontrib>Ishibashi, Minako</creatorcontrib><creatorcontrib>Charo, Israel F.</creatorcontrib><creatorcontrib>Sakamoto, Taiji</creatorcontrib><creatorcontrib>Murata, Toshinori</creatorcontrib><creatorcontrib>Ishibashi, Tatsuro</creatorcontrib><title>The critical role of ocular-infiltrating macrophages in the development of choroidal neovascularization</title><title>Journal of leukocyte biology</title><addtitle>J Leukoc Biol</addtitle><description>Choroidal neovascularization (CNV) is directly related to visual loss in some eye diseases, such as age‐related macular degeneration. Although several human histological studies have suggested the participation of macrophages in CNV formation, the precise mechanisms are still not fully understood. In this study, we elucidated the role of ocular‐infiltrating macrophages in experimental CNV using CCR2 knockout (KO) mice, wild‐type mice, and C57BL/6 (B6) mice. CCR2 is the receptor of monocyte chemoattractant protein‐1, and the number of infiltrating macrophage and the area of CNV were significantly reduced in CCR2 KO mice. Enriched ocular‐infiltrating macrophages from B6 mice actually showed angiogenic ability in a dorsal air sac assay. Moreover, their expression of class II, CD40, B7‐1 and B7‐2 molecules, and the mRNA for potential angiogenic factors, such as vascular endothelial growth factor, basic fibroblast growth factor, and tumor necrosis factor α, was also observed. Collectively, we conclude that ocular‐infiltrating macrophages play an important role in CNV generation.</description><subject>age-related macular degeneration</subject><subject>Animals</subject><subject>Cell Movement - immunology</subject><subject>Choroidal Neovascularization</subject><subject>Eye - blood supply</subject><subject>Eye - pathology</subject><subject>Growth Substances - biosynthesis</subject><subject>Growth Substances - genetics</subject><subject>Histocompatibility Antigens Class II - biosynthesis</subject><subject>Macrophages - physiology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>photocoagulation</subject><subject>Receptors, CCR2</subject><subject>Receptors, Chemokine</subject><subject>retinal pigment epithelium</subject><subject>RNA, Messenger - analysis</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFP3DAQRq2qqGyhp96rHKpeqsDYjp34SBG0VCtxgbPlOJONkRNv7SwR_PoadqXe6MmX970Zz0fIZwpnlDbq_MG3Z6CAVVy-IyuqeFNyWfP3ZAV1RUtRARyTjyk9AABnEj6QY8oannm1Ipu7AQsb3eys8UUMHovQF8HuvImlm3rn52hmN22K0dgYtoPZYCrcVMw51-Ej-rAdcZpfUnYIMbgueyYMjya9StxzjofplBz1xif8dHhPyP311d3lr3J9-_Pm8mJd2qpRomx7VmNrbNd20hghZCcrSytQleJW1VJYJtsalQSUtG-YMlz00EkqpGLYG35Cvu292xj-7DDNenTJovcm77RLuub5NgD0vyBVjAspeQa_78H8_ZQi9nob3Wjik6agXwrQuQB9KCDTXw7aXTti9489XDwDsAcW5_HpLZf-vf4BwESOfN1HBrcZFhdRp9F4nycwvSxLXWmqM_YXfaeeaA</recordid><startdate>200307</startdate><enddate>200307</enddate><creator>Tsutsumi, Chikako</creator><creator>Sonoda, Koh‐Hei</creator><creator>Egashira, Kensuke</creator><creator>Qiao, Hong</creator><creator>Hisatomi, Toshio</creator><creator>Nakao, Shintaro</creator><creator>Ishibashi, Minako</creator><creator>Charo, Israel F.</creator><creator>Sakamoto, Taiji</creator><creator>Murata, Toshinori</creator><creator>Ishibashi, Tatsuro</creator><general>Society for Leukocyte Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200307</creationdate><title>The critical role of ocular-infiltrating macrophages in the development of choroidal neovascularization</title><author>Tsutsumi, Chikako ; Sonoda, Koh‐Hei ; Egashira, Kensuke ; Qiao, Hong ; Hisatomi, Toshio ; Nakao, Shintaro ; Ishibashi, Minako ; Charo, Israel F. ; Sakamoto, Taiji ; Murata, Toshinori ; Ishibashi, Tatsuro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4895-bf27ebacdbd6aa556d64c1409493c9765c26b7e960e61f829a35f0d615692efa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>age-related macular degeneration</topic><topic>Animals</topic><topic>Cell Movement - immunology</topic><topic>Choroidal Neovascularization</topic><topic>Eye - blood supply</topic><topic>Eye - pathology</topic><topic>Growth Substances - biosynthesis</topic><topic>Growth Substances - genetics</topic><topic>Histocompatibility Antigens Class II - biosynthesis</topic><topic>Macrophages - physiology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>photocoagulation</topic><topic>Receptors, CCR2</topic><topic>Receptors, Chemokine</topic><topic>retinal pigment epithelium</topic><topic>RNA, Messenger - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsutsumi, Chikako</creatorcontrib><creatorcontrib>Sonoda, Koh‐Hei</creatorcontrib><creatorcontrib>Egashira, Kensuke</creatorcontrib><creatorcontrib>Qiao, Hong</creatorcontrib><creatorcontrib>Hisatomi, Toshio</creatorcontrib><creatorcontrib>Nakao, Shintaro</creatorcontrib><creatorcontrib>Ishibashi, Minako</creatorcontrib><creatorcontrib>Charo, Israel F.</creatorcontrib><creatorcontrib>Sakamoto, Taiji</creatorcontrib><creatorcontrib>Murata, Toshinori</creatorcontrib><creatorcontrib>Ishibashi, Tatsuro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of leukocyte biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsutsumi, Chikako</au><au>Sonoda, Koh‐Hei</au><au>Egashira, Kensuke</au><au>Qiao, Hong</au><au>Hisatomi, Toshio</au><au>Nakao, Shintaro</au><au>Ishibashi, Minako</au><au>Charo, Israel F.</au><au>Sakamoto, Taiji</au><au>Murata, Toshinori</au><au>Ishibashi, Tatsuro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The critical role of ocular-infiltrating macrophages in the development of choroidal neovascularization</atitle><jtitle>Journal of leukocyte biology</jtitle><addtitle>J Leukoc Biol</addtitle><date>2003-07</date><risdate>2003</risdate><volume>74</volume><issue>1</issue><spage>25</spage><epage>32</epage><pages>25-32</pages><issn>0741-5400</issn><eissn>1938-3673</eissn><abstract>Choroidal neovascularization (CNV) is directly related to visual loss in some eye diseases, such as age‐related macular degeneration. Although several human histological studies have suggested the participation of macrophages in CNV formation, the precise mechanisms are still not fully understood. In this study, we elucidated the role of ocular‐infiltrating macrophages in experimental CNV using CCR2 knockout (KO) mice, wild‐type mice, and C57BL/6 (B6) mice. CCR2 is the receptor of monocyte chemoattractant protein‐1, and the number of infiltrating macrophage and the area of CNV were significantly reduced in CCR2 KO mice. Enriched ocular‐infiltrating macrophages from B6 mice actually showed angiogenic ability in a dorsal air sac assay. Moreover, their expression of class II, CD40, B7‐1 and B7‐2 molecules, and the mRNA for potential angiogenic factors, such as vascular endothelial growth factor, basic fibroblast growth factor, and tumor necrosis factor α, was also observed. Collectively, we conclude that ocular‐infiltrating macrophages play an important role in CNV generation.</abstract><cop>United States</cop><pub>Society for Leukocyte Biology</pub><pmid>12832439</pmid><doi>10.1189/jlb.0902436</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0741-5400 |
| ispartof | Journal of leukocyte biology, 2003-07, Vol.74 (1), p.25-32 |
| issn | 0741-5400 1938-3673 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73367001 |
| source | MEDLINE; Wiley Online Library; Oxford Academic Journals (OUP); EZB Electronic Journals Library |
| subjects | age-related macular degeneration Animals Cell Movement - immunology Choroidal Neovascularization Eye - blood supply Eye - pathology Growth Substances - biosynthesis Growth Substances - genetics Histocompatibility Antigens Class II - biosynthesis Macrophages - physiology Male Mice Mice, Inbred C57BL Mice, Knockout photocoagulation Receptors, CCR2 Receptors, Chemokine retinal pigment epithelium RNA, Messenger - analysis |
| title | The critical role of ocular-infiltrating macrophages in the development of choroidal neovascularization |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T19%3A44%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20critical%20role%20of%20ocular-infiltrating%20macrophages%20in%20the%20development%20of%20choroidal%20neovascularization&rft.jtitle=Journal%20of%20leukocyte%20biology&rft.au=Tsutsumi,%20Chikako&rft.date=2003-07&rft.volume=74&rft.issue=1&rft.spage=25&rft.epage=32&rft.pages=25-32&rft.issn=0741-5400&rft.eissn=1938-3673&rft_id=info:doi/10.1189/jlb.0902436&rft_dat=%3Cproquest_cross%3E73367001%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19235663&rft_id=info:pmid/12832439&rfr_iscdi=true |