ESI-MS study on non-covalent bond complex of rhFKBP12 and new neurogrowth promoter

ESI-MS study on non-covalent bond complex of rhFKBP12 and new neurogrowth promoter

An ESI-MS method for studying the non-covalent bond complex of rhFKBP12 with its nonimmunosuppressive ligands was developed. The method was used to screen out three compounds capable of binding to rhFKBP12 non-covalently from 52 compounds. By competing binding experiment, the binding site and the re...

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Veröffentlicht in:Science China. Life sciences 2003-06, Vol.46 (3), p.286-292
Hauptverfasser: Wang, Hongxia, Zhang, Xuemin, Yang, Songcheng, Xiao, Junhai, Nie, Aihua, Zhao, Liqin, Li, Song
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Tacrolimus
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container_issue 3
container_start_page 286
container_title Science China. Life sciences
container_volume 46
creator Wang, Hongxia
Zhang, Xuemin
Yang, Songcheng
Xiao, Junhai
Nie, Aihua
Zhao, Liqin
Li, Song
description An ESI-MS method for studying the non-covalent bond complex of rhFKBP12 with its nonimmunosuppressive ligands was developed. The method was used to screen out three compounds capable of binding to rhFKBP12 non-covalently from 52 compounds. By competing binding experiment, the binding site and the relative binding strength of these three compounds 000107, 000308 and A2B12 with rhFKBP12 were measured. All of them have the same binding site as FK506 does. X-ray crystalline diffraction experiment of non-covalent bond complex of 000107, 000308 with rhFKBP12 by Tsinghua University showed the same results. Among them 000308 has good effect on stimulating neurite to grow in chicken sensory neuronal cultures.
doi_str_mv 10.1360/03yc9030
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subjects Tacrolimus
title ESI-MS study on non-covalent bond complex of rhFKBP12 and new neurogrowth promoter
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