Cell cycle dependency of rice alpha-amylase production in a recombinant yeast
The cell cycle dependency of rice alpha-amylase production in Saccharomyces cerevisiae was investigated using synchronous and arrested cultures. The results of two separate synchronous cultures, using alpha-mating factor and a cdc28 mutant, indicated that the rice alpha-amylase-specific production r...
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| Veröffentlicht in: | Biotechnology and bioengineering 1997-05, Vol.54 (3), p.262-271 |
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| creator | Uchiyama, K Morimoto, M Yokoyama, Y Shioya, S |
| description | The cell cycle dependency of rice alpha-amylase production in Saccharomyces cerevisiae was investigated using synchronous and arrested cultures. The results of two separate synchronous cultures, using alpha-mating factor and a cdc28 mutant, indicated that the rice alpha-amylase-specific production rate is not constant during the cell cycle. The specific production rates during G1, S, and M phases were then ascertained by inhibiting the progression of the cell cycle using alpha-mating factor, hydroxyurea, and nocodazole, respectively. The specific production rate was found to be maximal during the M phase. The increase in the specific production rate during the M phase was confirmed from the accumulation of M-phase cells using a cdc15 mutant. The intracellular content of rice alpha-amylase was also measured during the cell cycle. Like the specific production rate, the intracellular content was found to fluctuate throughout the cell cycle, and to reach a maximum during M phase. |
| doi_str_mv | 10.1002/(SICI)1097-0290(19970505)54:3<262::AID-BIT7>3.0.CO;2-D |
| format | Article |
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The results of two separate synchronous cultures, using alpha-mating factor and a cdc28 mutant, indicated that the rice alpha-amylase-specific production rate is not constant during the cell cycle. The specific production rates during G1, S, and M phases were then ascertained by inhibiting the progression of the cell cycle using alpha-mating factor, hydroxyurea, and nocodazole, respectively. The specific production rate was found to be maximal during the M phase. The increase in the specific production rate during the M phase was confirmed from the accumulation of M-phase cells using a cdc15 mutant. The intracellular content of rice alpha-amylase was also measured during the cell cycle. Like the specific production rate, the intracellular content was found to fluctuate throughout the cell cycle, and to reach a maximum during M phase.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/(SICI)1097-0290(19970505)54:3<262::AID-BIT7>3.0.CO;2-D</identifier><identifier>PMID: 18634092</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>arrested culture ; Biological and medical sciences ; Biotechnology ; cell cycle dependency ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; Oryza sativa ; plant biochemistry ; plant breeding ; plant genetics ; plant physiology ; recombinant yeast ; rice α-amylase ; Saccharomyces cerevisiae ; synchronous culture</subject><ispartof>Biotechnology and bioengineering, 1997-05, Vol.54 (3), p.262-271</ispartof><rights>Copyright © 1997 John Wiley & Sons, Inc.</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c6337-555a526fb8fc03785b0eb98365b0b0d1ea6ca2865803805fe4a56740cbb1a7153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291097-0290%2819970505%2954%3A3%3C262%3A%3AAID-BIT7%3E3.0.CO%3B2-D$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291097-0290%2819970505%2954%3A3%3C262%3A%3AAID-BIT7%3E3.0.CO%3B2-D$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2669219$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18634092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Uchiyama, K</creatorcontrib><creatorcontrib>Morimoto, M</creatorcontrib><creatorcontrib>Yokoyama, Y</creatorcontrib><creatorcontrib>Shioya, S</creatorcontrib><title>Cell cycle dependency of rice alpha-amylase production in a recombinant yeast</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>The cell cycle dependency of rice alpha-amylase production in Saccharomyces cerevisiae was investigated using synchronous and arrested cultures. The results of two separate synchronous cultures, using alpha-mating factor and a cdc28 mutant, indicated that the rice alpha-amylase-specific production rate is not constant during the cell cycle. The specific production rates during G1, S, and M phases were then ascertained by inhibiting the progression of the cell cycle using alpha-mating factor, hydroxyurea, and nocodazole, respectively. The specific production rate was found to be maximal during the M phase. The increase in the specific production rate during the M phase was confirmed from the accumulation of M-phase cells using a cdc15 mutant. The intracellular content of rice alpha-amylase was also measured during the cell cycle. Like the specific production rate, the intracellular content was found to fluctuate throughout the cell cycle, and to reach a maximum during M phase.</description><subject>arrested culture</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cell cycle dependency</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>Oryza sativa</subject><subject>plant biochemistry</subject><subject>plant breeding</subject><subject>plant genetics</subject><subject>plant physiology</subject><subject>recombinant yeast</subject><subject>rice α-amylase</subject><subject>Saccharomyces cerevisiae</subject><subject>synchronous culture</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqNkdFu0zAUhiMEYmXwCpALBNtFyrEd20k3TdpSGBWFCnWDy6MT1xmBNClxK8jb49BSkEDAlX2kz7_-4y8IzhgMGQB_djSfZJNjBqmOgKdwxNJUgwR5LOOROOWKj0bnk3F0MbnSZ2IIw2x2wqPxrWCwf3I7GACAioRM-UFwz7mPftSJUneDA5YoEUPKB8HrzFZVaDpT2XBhV7Ze2Np0YVOEbWlsSNXqA0W07CpyNly1zWJj1mVTh2UdUtha0yzzsqZ6HXaW3Pp-cKegytkHu_MwuH7x_Cp7GU1nl5PsfBoZJYSOpJQkuSrypDAgdCJzsHmaCOUvOSyYJWWIJ0omIBKQhY1JKh2DyXNGmklxGDzd5vpGnzfWrXFZOuM3odo2G4daCKUUY7Enn_yVZMp_N8TiP0AQsYLUg--2oGkb51pb4Kotl9R2yAB7d4i9O-xFYC8Cf7hDGaNA7w7Ru8PenZ8BsxlyHPvgh7sGm3xpFz9jd7I88HgHkDNUFS3VpnR7jiuVcvZLwS9lZbvf2v2z3B-6fZ99cLQNLt3aft0HU_sJlRZa4vs3l3iRTOXbecrwlecfbfmCGqSb1ne9nnNgArxbb0iKb2bT1yQ</recordid><startdate>19970505</startdate><enddate>19970505</enddate><creator>Uchiyama, K</creator><creator>Morimoto, M</creator><creator>Yokoyama, Y</creator><creator>Shioya, S</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19970505</creationdate><title>Cell cycle dependency of rice alpha-amylase production in a recombinant yeast</title><author>Uchiyama, K ; Morimoto, M ; Yokoyama, Y ; Shioya, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6337-555a526fb8fc03785b0eb98365b0b0d1ea6ca2865803805fe4a56740cbb1a7153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>arrested culture</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>cell cycle dependency</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial engineering. Fermentation and microbial culture technology</topic><topic>Oryza sativa</topic><topic>plant biochemistry</topic><topic>plant breeding</topic><topic>plant genetics</topic><topic>plant physiology</topic><topic>recombinant yeast</topic><topic>rice α-amylase</topic><topic>Saccharomyces cerevisiae</topic><topic>synchronous culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Uchiyama, K</creatorcontrib><creatorcontrib>Morimoto, M</creatorcontrib><creatorcontrib>Yokoyama, Y</creatorcontrib><creatorcontrib>Shioya, S</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uchiyama, K</au><au>Morimoto, M</au><au>Yokoyama, Y</au><au>Shioya, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell cycle dependency of rice alpha-amylase production in a recombinant yeast</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>1997-05-05</date><risdate>1997</risdate><volume>54</volume><issue>3</issue><spage>262</spage><epage>271</epage><pages>262-271</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>The cell cycle dependency of rice alpha-amylase production in Saccharomyces cerevisiae was investigated using synchronous and arrested cultures. The results of two separate synchronous cultures, using alpha-mating factor and a cdc28 mutant, indicated that the rice alpha-amylase-specific production rate is not constant during the cell cycle. The specific production rates during G1, S, and M phases were then ascertained by inhibiting the progression of the cell cycle using alpha-mating factor, hydroxyurea, and nocodazole, respectively. The specific production rate was found to be maximal during the M phase. The increase in the specific production rate during the M phase was confirmed from the accumulation of M-phase cells using a cdc15 mutant. 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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | arrested culture Biological and medical sciences Biotechnology cell cycle dependency Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Oryza sativa plant biochemistry plant breeding plant genetics plant physiology recombinant yeast rice α-amylase Saccharomyces cerevisiae synchronous culture |
| title | Cell cycle dependency of rice alpha-amylase production in a recombinant yeast |
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