Estimation of growth yield and maintenance coefficient of plant cell suspensions
Methodology is presented for the determination of growth yield (Y(g)) and maintenance coefficient (m) for carbon utilization of plant cells grown in suspension culture. Estimation of Y(g) and m requires measurements of specific growth rate and specific rate of substrate uptake (q) at different growt...
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| Veröffentlicht in: | Biotechnology and bioengineering 1991-12, Vol.38 (10), p.1131-1136 |
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| container_title | Biotechnology and bioengineering |
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| creator | Schnapp, S.R. (Purdue University, West Lafayette, IN) Curtis, W.R Bressan, R.A Hasegawa, P.M |
| description | Methodology is presented for the determination of growth yield (Y(g)) and maintenance coefficient (m) for carbon utilization of plant cells grown in suspension culture. Estimation of Y(g) and m requires measurements of specific growth rate and specific rate of substrate uptake (q) at different growth limiting substrate concentrations. Batch culture of tobacco cells did not permit evaluation of Y(g) and m because the specific growth rate is constant and maximal during most of the growth cycle. In batch culture, the period of declining specific growth rate is extremely brief because of the rapid transition from logarithmic growth to stationary phase. This occurs because the Km for growth is relatively small compared to the initial sucrose concentration. Thus, when the substrate level reaches the Km, the large mass of cells rapidly depletes the remaining substrate. In contrast, semicontinuous culture facilitates the determination of Y(g) and m because various steady-state growth rates can be achieved. Mathematical expressions were developed to determine the effective values of specific growth rate and q over the semicontinuous replacement interval. The validity of this approach was verified by conducting simulations using experimentally determined parameters |
| doi_str_mv | 10.1002/bit.260381003 |
| format | Article |
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(Purdue University, West Lafayette, IN) ; Curtis, W.R ; Bressan, R.A ; Hasegawa, P.M</creator><creatorcontrib>Schnapp, S.R. (Purdue University, West Lafayette, IN) ; Curtis, W.R ; Bressan, R.A ; Hasegawa, P.M</creatorcontrib><description>Methodology is presented for the determination of growth yield (Y(g)) and maintenance coefficient (m) for carbon utilization of plant cells grown in suspension culture. Estimation of Y(g) and m requires measurements of specific growth rate and specific rate of substrate uptake (q) at different growth limiting substrate concentrations. Batch culture of tobacco cells did not permit evaluation of Y(g) and m because the specific growth rate is constant and maximal during most of the growth cycle. In batch culture, the period of declining specific growth rate is extremely brief because of the rapid transition from logarithmic growth to stationary phase. This occurs because the Km for growth is relatively small compared to the initial sucrose concentration. Thus, when the substrate level reaches the Km, the large mass of cells rapidly depletes the remaining substrate. In contrast, semicontinuous culture facilitates the determination of Y(g) and m because various steady-state growth rates can be achieved. Mathematical expressions were developed to determine the effective values of specific growth rate and q over the semicontinuous replacement interval. The validity of this approach was verified by conducting simulations using experimentally determined parameters</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.260381003</identifier><identifier>PMID: 18600707</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Biological and medical sciences ; carbon utilization ; CARBONE ; CARBONO ; Cell physiology ; cell suspensions ; CELLULE ; CELULAS ; CRECIMIENTO ; CROISSANCE ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; ENERGIA ; ENERGIE ; Fundamental and applied biological sciences. Psychology ; General aspects, methods ; growth yield ; maintenance coefficient ; MODELE MATHEMATIQUE ; MODELOS MATEMATICOS ; NICOTIANA TABACUM ; plant cell suspensions ; Plant physiology and development ; sucrose ; suspension culture</subject><ispartof>Biotechnology and bioengineering, 1991-12, Vol.38 (10), p.1131-1136</ispartof><rights>Copyright © 1991 John Wiley & Sons, Inc.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5573-28540917917f0e99e05eacb2a198a9107389ef8b5eb01cd529898df16b484583</citedby><cites>FETCH-LOGICAL-c5573-28540917917f0e99e05eacb2a198a9107389ef8b5eb01cd529898df16b484583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.260381003$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.260381003$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5142476$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18600707$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schnapp, S.R. (Purdue University, West Lafayette, IN)</creatorcontrib><creatorcontrib>Curtis, W.R</creatorcontrib><creatorcontrib>Bressan, R.A</creatorcontrib><creatorcontrib>Hasegawa, P.M</creatorcontrib><title>Estimation of growth yield and maintenance coefficient of plant cell suspensions</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>Methodology is presented for the determination of growth yield (Y(g)) and maintenance coefficient (m) for carbon utilization of plant cells grown in suspension culture. Estimation of Y(g) and m requires measurements of specific growth rate and specific rate of substrate uptake (q) at different growth limiting substrate concentrations. Batch culture of tobacco cells did not permit evaluation of Y(g) and m because the specific growth rate is constant and maximal during most of the growth cycle. In batch culture, the period of declining specific growth rate is extremely brief because of the rapid transition from logarithmic growth to stationary phase. This occurs because the Km for growth is relatively small compared to the initial sucrose concentration. Thus, when the substrate level reaches the Km, the large mass of cells rapidly depletes the remaining substrate. In contrast, semicontinuous culture facilitates the determination of Y(g) and m because various steady-state growth rates can be achieved. Mathematical expressions were developed to determine the effective values of specific growth rate and q over the semicontinuous replacement interval. The validity of this approach was verified by conducting simulations using experimentally determined parameters</description><subject>Biological and medical sciences</subject><subject>carbon utilization</subject><subject>CARBONE</subject><subject>CARBONO</subject><subject>Cell physiology</subject><subject>cell suspensions</subject><subject>CELLULE</subject><subject>CELULAS</subject><subject>CRECIMIENTO</subject><subject>CROISSANCE</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>ENERGIA</subject><subject>ENERGIE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, methods</subject><subject>growth yield</subject><subject>maintenance coefficient</subject><subject>MODELE MATHEMATIQUE</subject><subject>MODELOS MATEMATICOS</subject><subject>NICOTIANA TABACUM</subject><subject>plant cell suspensions</subject><subject>Plant physiology and development</subject><subject>sucrose</subject><subject>suspension culture</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNp9kMtv1DAQhy0EokvLkQsHlAOip7RjO34doY-l0vKQWOBoOcm4GLLJYmfV7n-PVxstPVWyNDPS55mfPkJeUTijAOy8DuMZk8B1nvgTMqNgVAnMwFMyAwBZcmHYEXmR0u88Ki3lc3JEtcw9qBn5epXGsHJjGPpi8MVtHO7GX8U2YNcWrm-LlQv9iL3rGyyaAb0PTcB-3LHrzuWmwa4r0iatsU95STohz7zrEr6c6jFZXl8tLz6Wiy_zm4v3i7IRQvGSaVGBoSo_D2gMgkDX1MxRo52hoLg26HUtsAbatIIZbXTrqawrXQnNj8npfu06Dn83mEa7CmmXxfU4bJJVnEsphOSZfPcoSSVoqqTMYLkHmzikFNHbdcxq4tZSsDvXNru2B9eZfzMt3tQrbP_Tk9wMvJ0AlxrX-ZgthnTgBK1YpXZ31R67Cx1uHz9qP9wsHyaYEoc04v3hp4t_rFRcCfvz89xe_pgvLrVg9lPmX-957wbrbmMO8_2bqYBWUvN_He2tJQ</recordid><startdate>19911205</startdate><enddate>19911205</enddate><creator>Schnapp, S.R. 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Psychology</topic><topic>General aspects, methods</topic><topic>growth yield</topic><topic>maintenance coefficient</topic><topic>MODELE MATHEMATIQUE</topic><topic>MODELOS MATEMATICOS</topic><topic>NICOTIANA TABACUM</topic><topic>plant cell suspensions</topic><topic>Plant physiology and development</topic><topic>sucrose</topic><topic>suspension culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schnapp, S.R. 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Batch culture of tobacco cells did not permit evaluation of Y(g) and m because the specific growth rate is constant and maximal during most of the growth cycle. In batch culture, the period of declining specific growth rate is extremely brief because of the rapid transition from logarithmic growth to stationary phase. This occurs because the Km for growth is relatively small compared to the initial sucrose concentration. Thus, when the substrate level reaches the Km, the large mass of cells rapidly depletes the remaining substrate. In contrast, semicontinuous culture facilitates the determination of Y(g) and m because various steady-state growth rates can be achieved. Mathematical expressions were developed to determine the effective values of specific growth rate and q over the semicontinuous replacement interval. 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| ispartof | Biotechnology and bioengineering, 1991-12, Vol.38 (10), p.1131-1136 |
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| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences carbon utilization CARBONE CARBONO Cell physiology cell suspensions CELLULE CELULAS CRECIMIENTO CROISSANCE CULTIVO DE CELULAS CULTURE DE CELLULE ENERGIA ENERGIE Fundamental and applied biological sciences. Psychology General aspects, methods growth yield maintenance coefficient MODELE MATHEMATIQUE MODELOS MATEMATICOS NICOTIANA TABACUM plant cell suspensions Plant physiology and development sucrose suspension culture |
| title | Estimation of growth yield and maintenance coefficient of plant cell suspensions |
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