Precolumn derivatization of nucleotides based on fluorescent carbamate formation on the sugar moieties in high-performance liquid chromatography

The fluorescence derivatization of nucleotides with 2-(5-chlorocarbonyl-2-oxazolyl)-5,6-methylenedioxybenzo++ furan in the presence of sodium azide and the separation of the derivatives by high-performance liquid chromatography are described. The reagent reacts with 5'-terminal hydroxyl groups...

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Veröffentlicht in:	Chemical & pharmaceutical bulletin 1992, Vol.40 (9), p.2559-2561
Hauptverfasser:	NAGAOKA, H, NOHTA, H, SAITO, M, OHKURA, Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Base Sequence Carbamates Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid Exact sciences and technology Fluorescent Dyes Molecular Sequence Data Oligonucleotides - analysis Other chromatographic methods
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