On-line estimation of viable cells in a hybridoma culture at various DO levels using ATP balancing and redox potential measurement

Two on‐line methods for the estimation of viable cell number in hybridoma cultivation were investigated. One used an empirical correlation between redox potential and animal cell density. The other was based on an ATP balance with ATP steady‐state assumption. Oxygen uptake rate measurement provided...

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Veröffentlicht in:	Biotechnology and bioengineering 1996-02, Vol.49 (3), p.277-283
Hauptverfasser:	Eyer, Kurt, Heinzle, Elmar
Format:	Artikel
Sprache:	eng
Schlagworte:	Aeration Animal cells ATP balance Biological and medical sciences Biotechnology cell culture Cell hybridization, cell fusion, hybridomas Correlation Culture dissolved oxygen Electrode potentials Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology hybridoma Lactates Methods. Procedures. Technologies on-line viable cell concentration Oxygen consumption redox potential Saturation Uptakes
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