Molecular assembly of proteins and conjugated polymers: toward development of biosensors
A molecular assembly in which a conjugated polymer is interfaced with a photodynamic protein is described. The conjugated polymer, functionalized with biotin, is designed such that it can be physisorbed on or chemically grown off a glass surface. The streptavidin-derivatized protein is immobilized o...
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| Veröffentlicht in: | Biotechnology and bioengineering 1995-01, Vol.45 (2), p.116-121 |
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| container_title | Biotechnology and bioengineering |
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| creator | Ayyagari, M.S. (University of Massachusetts-Lowell, Lowell, MA.) Pande, R Kamtekar, S Gao, H Marx, K.A Kumar, J Tripathy, S.K Akkara, J.A Kaplan, D.L |
| description | A molecular assembly in which a conjugated polymer is interfaced with a photodynamic protein is described. The conjugated polymer, functionalized with biotin, is designed such that it can be physisorbed on or chemically grown off a glass surface. The streptavidin-derivatized protein is immobilized on the biotinylated polymer matrix through the strong biotin-streptavidin interactions. The assembly, built on the surface of an optical fiber or on the inside walls of a glass capillary, forms an integral part of a biosensor for the detection of environmental pollutants such as organophosphorus-based insecticides. The protein in the system can be replaced by any biological macromolecule of interest. We study one specific case, the inhibition of the enzyme alkaline phosphatase. The enzyme catalyzes a reaction producing an intermediate compound that chemiluminesces, and the chemiluminescence signal is monitored to detect and quantify insecticides such as paraoxon and methyl parathion. Preliminary results indicate ppb level detection with response time less than 1 minute |
| doi_str_mv | 10.1002/bit.260450204 |
| format | Article |
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(University of Massachusetts-Lowell, Lowell, MA.) ; Pande, R ; Kamtekar, S ; Gao, H ; Marx, K.A ; Kumar, J ; Tripathy, S.K ; Akkara, J.A ; Kaplan, D.L</creator><creatorcontrib>Ayyagari, M.S. (University of Massachusetts-Lowell, Lowell, MA.) ; Pande, R ; Kamtekar, S ; Gao, H ; Marx, K.A ; Kumar, J ; Tripathy, S.K ; Akkara, J.A ; Kaplan, D.L</creatorcontrib><description>A molecular assembly in which a conjugated polymer is interfaced with a photodynamic protein is described. The conjugated polymer, functionalized with biotin, is designed such that it can be physisorbed on or chemically grown off a glass surface. The streptavidin-derivatized protein is immobilized on the biotinylated polymer matrix through the strong biotin-streptavidin interactions. The assembly, built on the surface of an optical fiber or on the inside walls of a glass capillary, forms an integral part of a biosensor for the detection of environmental pollutants such as organophosphorus-based insecticides. The protein in the system can be replaced by any biological macromolecule of interest. We study one specific case, the inhibition of the enzyme alkaline phosphatase. The enzyme catalyzes a reaction producing an intermediate compound that chemiluminesces, and the chemiluminescence signal is monitored to detect and quantify insecticides such as paraoxon and methyl parathion. Preliminary results indicate ppb level detection with response time less than 1 minute</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.260450204</identifier><identifier>PMID: 18623092</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>alkaline phosphatase ; BIODETECTEUR ; Biological and medical sciences ; biosensor ; BIOSENSORES ; Biosensors ; Biotechnology ; BIOTINA ; BIOTINE ; Fundamental and applied biological sciences. Psychology ; IMMOBILISATION ; immobilization and chemiluminescence ; INMOVILIZACION ; INSECTICIDAS ; INSECTICIDE ; insecticides ; Methods. Procedures. Technologies ; phycoerythrin ; POLIMEROS ; POLYMERE ; PROPIEDADES OPTICAS ; PROPRIETE OPTIQUE ; PROTEINAS ; PROTEINE ; Various methods and equipments</subject><ispartof>Biotechnology and bioengineering, 1995-01, Vol.45 (2), p.116-121</ispartof><rights>Copyright © 1995 John Wiley & Sons, Inc.</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5284-220183a3a71a963e3fc28c4f97319dfbaca2ddadf59ecbf39f6261455d21aa163</citedby><cites>FETCH-LOGICAL-c5284-220183a3a71a963e3fc28c4f97319dfbaca2ddadf59ecbf39f6261455d21aa163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.260450204$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.260450204$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27928,27929,45578,45579</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3348703$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18623092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ayyagari, M.S. (University of Massachusetts-Lowell, Lowell, MA.)</creatorcontrib><creatorcontrib>Pande, R</creatorcontrib><creatorcontrib>Kamtekar, S</creatorcontrib><creatorcontrib>Gao, H</creatorcontrib><creatorcontrib>Marx, K.A</creatorcontrib><creatorcontrib>Kumar, J</creatorcontrib><creatorcontrib>Tripathy, S.K</creatorcontrib><creatorcontrib>Akkara, J.A</creatorcontrib><creatorcontrib>Kaplan, D.L</creatorcontrib><title>Molecular assembly of proteins and conjugated polymers: toward development of biosensors</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>A molecular assembly in which a conjugated polymer is interfaced with a photodynamic protein is described. The conjugated polymer, functionalized with biotin, is designed such that it can be physisorbed on or chemically grown off a glass surface. The streptavidin-derivatized protein is immobilized on the biotinylated polymer matrix through the strong biotin-streptavidin interactions. The assembly, built on the surface of an optical fiber or on the inside walls of a glass capillary, forms an integral part of a biosensor for the detection of environmental pollutants such as organophosphorus-based insecticides. The protein in the system can be replaced by any biological macromolecule of interest. We study one specific case, the inhibition of the enzyme alkaline phosphatase. The enzyme catalyzes a reaction producing an intermediate compound that chemiluminesces, and the chemiluminescence signal is monitored to detect and quantify insecticides such as paraoxon and methyl parathion. Preliminary results indicate ppb level detection with response time less than 1 minute</description><subject>alkaline phosphatase</subject><subject>BIODETECTEUR</subject><subject>Biological and medical sciences</subject><subject>biosensor</subject><subject>BIOSENSORES</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>BIOTINA</subject><subject>BIOTINE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>IMMOBILISATION</subject><subject>immobilization and chemiluminescence</subject><subject>INMOVILIZACION</subject><subject>INSECTICIDAS</subject><subject>INSECTICIDE</subject><subject>insecticides</subject><subject>Methods. Procedures. Technologies</subject><subject>phycoerythrin</subject><subject>POLIMEROS</subject><subject>POLYMERE</subject><subject>PROPIEDADES OPTICAS</subject><subject>PROPRIETE OPTIQUE</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Various methods and equipments</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNqF0r1v1DAYBnALgehRGFkYUAYEU8prO7HjblDRo1IBVbSim_XGH1VKEh92Qrn_Hh93OpjKZFn6vfZjPSbkOYUjCsDett10xARUNTCoHpAFBSVLYAoekgUAiJLXih2QJynd5q1shHhMDmgjGAfFFuT6U-idmXuMBabkhrZfF8EXqxgm142pwNEWJoy38w1Ozhar0K8HF9NxMYU7jLaw7qfrw2pw47SZa7uQ3JhCTE_JI499cs926yG5Ov1wefKxPP-yPDt5d16amjVVyRjQhiNHSVEJ7rg3rDGVV5JTZX2LBpm1aH2tnGk9V14wQau6towiUsEPyZvtuTnyj9mlSQ9dMq7vcXRhTlpyLgSjimf5-l5JJVR_5H-hUFxwxTIst9DEkFJ0Xq9iN2Bcawp6047O7eh9O9m_3B08t4Ozf_Wujgxe7QAmg72POJou7R3nVSNh8xK5ZXdd79b3X6rfn13-m2CXuEuT-7WfxPhdC8llrb99XuoLuL7g9BT0MvsXW-8xaLyJOczVVyVA5X_EfwO-Rr9u</recordid><startdate>19950120</startdate><enddate>19950120</enddate><creator>Ayyagari, M.S. (University of Massachusetts-Lowell, Lowell, MA.)</creator><creator>Pande, R</creator><creator>Kamtekar, S</creator><creator>Gao, H</creator><creator>Marx, K.A</creator><creator>Kumar, J</creator><creator>Tripathy, S.K</creator><creator>Akkara, J.A</creator><creator>Kaplan, D.L</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19950120</creationdate><title>Molecular assembly of proteins and conjugated polymers: toward development of biosensors</title><author>Ayyagari, M.S. (University of Massachusetts-Lowell, Lowell, MA.) ; Pande, R ; Kamtekar, S ; Gao, H ; Marx, K.A ; Kumar, J ; Tripathy, S.K ; Akkara, J.A ; Kaplan, D.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5284-220183a3a71a963e3fc28c4f97319dfbaca2ddadf59ecbf39f6261455d21aa163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>alkaline phosphatase</topic><topic>BIODETECTEUR</topic><topic>Biological and medical sciences</topic><topic>biosensor</topic><topic>BIOSENSORES</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>BIOTINA</topic><topic>BIOTINE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>IMMOBILISATION</topic><topic>immobilization and chemiluminescence</topic><topic>INMOVILIZACION</topic><topic>INSECTICIDAS</topic><topic>INSECTICIDE</topic><topic>insecticides</topic><topic>Methods. Procedures. Technologies</topic><topic>phycoerythrin</topic><topic>POLIMEROS</topic><topic>POLYMERE</topic><topic>PROPIEDADES OPTICAS</topic><topic>PROPRIETE OPTIQUE</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ayyagari, M.S. (University of Massachusetts-Lowell, Lowell, MA.)</creatorcontrib><creatorcontrib>Pande, R</creatorcontrib><creatorcontrib>Kamtekar, S</creatorcontrib><creatorcontrib>Gao, H</creatorcontrib><creatorcontrib>Marx, K.A</creatorcontrib><creatorcontrib>Kumar, J</creatorcontrib><creatorcontrib>Tripathy, S.K</creatorcontrib><creatorcontrib>Akkara, J.A</creatorcontrib><creatorcontrib>Kaplan, D.L</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ayyagari, M.S. (University of Massachusetts-Lowell, Lowell, MA.)</au><au>Pande, R</au><au>Kamtekar, S</au><au>Gao, H</au><au>Marx, K.A</au><au>Kumar, J</au><au>Tripathy, S.K</au><au>Akkara, J.A</au><au>Kaplan, D.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular assembly of proteins and conjugated polymers: toward development of biosensors</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>1995-01-20</date><risdate>1995</risdate><volume>45</volume><issue>2</issue><spage>116</spage><epage>121</epage><pages>116-121</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>A molecular assembly in which a conjugated polymer is interfaced with a photodynamic protein is described. The conjugated polymer, functionalized with biotin, is designed such that it can be physisorbed on or chemically grown off a glass surface. The streptavidin-derivatized protein is immobilized on the biotinylated polymer matrix through the strong biotin-streptavidin interactions. The assembly, built on the surface of an optical fiber or on the inside walls of a glass capillary, forms an integral part of a biosensor for the detection of environmental pollutants such as organophosphorus-based insecticides. The protein in the system can be replaced by any biological macromolecule of interest. We study one specific case, the inhibition of the enzyme alkaline phosphatase. The enzyme catalyzes a reaction producing an intermediate compound that chemiluminesces, and the chemiluminescence signal is monitored to detect and quantify insecticides such as paraoxon and methyl parathion. Preliminary results indicate ppb level detection with response time less than 1 minute</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18623092</pmid><doi>10.1002/bit.260450204</doi><tpages>6</tpages></addata></record> |
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| subjects | alkaline phosphatase BIODETECTEUR Biological and medical sciences biosensor BIOSENSORES Biosensors Biotechnology BIOTINA BIOTINE Fundamental and applied biological sciences. Psychology IMMOBILISATION immobilization and chemiluminescence INMOVILIZACION INSECTICIDAS INSECTICIDE insecticides Methods. Procedures. Technologies phycoerythrin POLIMEROS POLYMERE PROPIEDADES OPTICAS PROPRIETE OPTIQUE PROTEINAS PROTEINE Various methods and equipments |
| title | Molecular assembly of proteins and conjugated polymers: toward development of biosensors |
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