Polyoxazoline-Peptide adducts that retain antibody avidity
Synthetic polymers have long been used to modify various properties of proteins such as activity and solubility. Polyethylene glycol (PEG) has been widely used to form adducts with enzymes and antibodies. In this study, the polyoxazoline family of water‐soluble polymers was used to synthesize adduct...
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| Veröffentlicht in: | Biotechnology and bioengineering 1992-04, Vol.39 (10), p.1024-1030 |
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| container_title | Biotechnology and bioengineering |
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| creator | Velander, William H. Madurawe, Rapti D. Subramanian, Anuradha Kumar, Guneet Sinai-Zingde, Gurudas Riffle, Judy S. Orthner, Carolyn L. |
| description | Synthetic polymers have long been used to modify various properties of proteins such as activity and solubility. Polyethylene glycol (PEG) has been widely used to form adducts with enzymes and antibodies. In this study, the polyoxazoline family of water‐soluble polymers was used to synthesize adducts containing a synthetic peptide recognized by a monoclonal antibody (MAb) directed against human protein C (hPC). This is the first application of direct conjugation of unterminated or “living” polymer to a peptide. The avidity of the antibody for the various adducts was characterized with respect to size and hydrophilicity of methyl‐ and ethyl‐substituted polyoxazoline polymers (POX). Avidity of the adducts was not found to be dependent upon the hydrophilicity and was slightly decreased due to polymer modification. The methyl‐POX‐peptide adducts were found to be highly water soluble, while the ethyl‐POX‐peptide adducts showed sporadic problems with aqueous solubility. Because the polymer‐peptide adducts retained avidity for the antibody, polyoxazoline polymers may have potential application to protein‐adduct chemistry. |
| doi_str_mv | 10.1002/bit.260391006 |
| format | Article |
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Because the polymer‐peptide adducts retained avidity for the antibody, polyoxazoline polymers may have potential application to protein‐adduct chemistry.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.260391006</identifier><identifier>PMID: 18600901</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. 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Bioeng</addtitle><description>Synthetic polymers have long been used to modify various properties of proteins such as activity and solubility. Polyethylene glycol (PEG) has been widely used to form adducts with enzymes and antibodies. In this study, the polyoxazoline family of water‐soluble polymers was used to synthesize adducts containing a synthetic peptide recognized by a monoclonal antibody (MAb) directed against human protein C (hPC). This is the first application of direct conjugation of unterminated or “living” polymer to a peptide. The avidity of the antibody for the various adducts was characterized with respect to size and hydrophilicity of methyl‐ and ethyl‐substituted polyoxazoline polymers (POX). Avidity of the adducts was not found to be dependent upon the hydrophilicity and was slightly decreased due to polymer modification. The methyl‐POX‐peptide adducts were found to be highly water soluble, while the ethyl‐POX‐peptide adducts showed sporadic problems with aqueous solubility. Because the polymer‐peptide adducts retained avidity for the antibody, polyoxazoline polymers may have potential application to protein‐adduct chemistry.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. 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| ispartof | Biotechnology and bioengineering, 1992-04, Vol.39 (10), p.1024-1030 |
| issn | 0006-3592 1097-0290 |
| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies monoclonal antibodies Others polymer-peptide adducts polyoxazoline Various methods and equipments |
| title | Polyoxazoline-Peptide adducts that retain antibody avidity |
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