Continuous 2-Keto-l-gulonic acid fermentation by mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium or Xanthomonas maltophilia
The fermentation process of 2-keto-L-gulonic acid (2KGA) from L-sorbose was developed using a two-stage continuous fermentation system. The mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium DSM 4026 produced 90 g/L of 2KGA from 120 g/L of L-sorbose at the dilution rate of...
Gespeichert in:
Veröffentlicht in: | Applied microbiology and biotechnology 2010-03, Vol.86 (2), p.469-480 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 480 |
---|---|
container_issue | 2 |
container_start_page | 469 |
container_title | Applied microbiology and biotechnology |
container_volume | 86 |
creator | Takagi, Yoshinori Sugisawa, Teruhide Hoshino, Tatsuo |
description | The fermentation process of 2-keto-L-gulonic acid (2KGA) from L-sorbose was developed using a two-stage continuous fermentation system. The mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium DSM 4026 produced 90 g/L of 2KGA from 120 g/L of L-sorbose at the dilution rate of 0.01 h⁻¹ in a single-stage continuous fermentation process. But after the production period was beyond 150 h, the significant decrease of 2KGA productivity was observed. When the non-spore forming bacteria Xanthomonas maltophilia IFO 12692 was used instead of B. megaterium DSM 4026 as a partner strain for K. vulgare DSM 4025, the 2KGA productivity was significantly improved in a two-stage continuous culture mode, in which two fermentors of the same size and volume were connected in series. In this mode, with two sets of 3-L jar fermentors, the steady state could be continued to over 1,331.5 h at least, when the dilution rates were 0.0382 h⁻¹ and 0.0380 hour⁻¹, respectively, for the first and second fermentors. The overall productivity was calculated to be 2.15 g/L/h at 113.1 g/L and a molar conversion yield of 90.1%. In the up-scaling fermentation to 30-L jar fermentors, 118.5 g/L of 2KGA was produced when dilution rates in both stages were 0.0430 hour⁻¹, and the overall productivity was calculated to be 2.55 g/L/h. |
doi_str_mv | 10.1007/s00253-009-2312-1 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733658977</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2036717741</sourcerecordid><originalsourceid>FETCH-LOGICAL-c461t-9baca47cad3bfa697e67197ebc97aef050183a40449ace92f0589d5c4dbe30db3</originalsourceid><addsrcrecordid>eNp9kc-KFDEQxoMo7jj6AF40COIpWklnOt1HHf_iiod1wVuoTqd7s6STMekW9018XNPM4IIHLylI_b6vivoIeczhJQdQrzKA2FUMoGWi4oLxO2TDZSUY1FzeJRvgasfUrm3OyIOcrwG4aOr6PjnjbQtCgNqQ3_sYZheWuGQq2Gc7R-bZuPgYnKFoXE8HmyYbZpxdDLS7oZP7ZXtqFj8vydI40FV0UrjRBrdM9OfiRyzdtxdfqCw7Ugw9fVPsvC9zJjvibNMKxkS_Y5iv4hQDlg76OR6unHf4kNwb0Gf76FS35PL9u2_7j-z864dP-9fnzMiaz6zt0KBUBvuqG7Bula0VL29nWoV2gB3wpkIJUrZobCvKT9P2OyP7zlbQd9WWvDj6HlL8sdg868llY73HYMtRtKqqukiUKuSzf8jruKRQltOiaSTUTWG3hB8hk2LOyQ76kNyE6UZz0Gto-hiaLqHpNTTNi-bJyXjpJtvfKk4pFeD5CcBs0A8Jg3H5LyeEVHUDq5E4crm0wmjT7Yb_m_70KBowahxTMb68EMWtXA5qELz6A5xkuhc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>288406873</pqid></control><display><type>article</type><title>Continuous 2-Keto-l-gulonic acid fermentation by mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium or Xanthomonas maltophilia</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Takagi, Yoshinori ; Sugisawa, Teruhide ; Hoshino, Tatsuo</creator><creatorcontrib>Takagi, Yoshinori ; Sugisawa, Teruhide ; Hoshino, Tatsuo</creatorcontrib><description>The fermentation process of 2-keto-L-gulonic acid (2KGA) from L-sorbose was developed using a two-stage continuous fermentation system. The mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium DSM 4026 produced 90 g/L of 2KGA from 120 g/L of L-sorbose at the dilution rate of 0.01 h⁻¹ in a single-stage continuous fermentation process. But after the production period was beyond 150 h, the significant decrease of 2KGA productivity was observed. When the non-spore forming bacteria Xanthomonas maltophilia IFO 12692 was used instead of B. megaterium DSM 4026 as a partner strain for K. vulgare DSM 4025, the 2KGA productivity was significantly improved in a two-stage continuous culture mode, in which two fermentors of the same size and volume were connected in series. In this mode, with two sets of 3-L jar fermentors, the steady state could be continued to over 1,331.5 h at least, when the dilution rates were 0.0382 h⁻¹ and 0.0380 hour⁻¹, respectively, for the first and second fermentors. The overall productivity was calculated to be 2.15 g/L/h at 113.1 g/L and a molar conversion yield of 90.1%. In the up-scaling fermentation to 30-L jar fermentors, 118.5 g/L of 2KGA was produced when dilution rates in both stages were 0.0430 hour⁻¹, and the overall productivity was calculated to be 2.55 g/L/h.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-009-2312-1</identifier><identifier>PMID: 19902207</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Acids ; Bacillus megaterium - metabolism ; Bacteria ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnological Products and Process Engineering ; Biotechnology ; Cell culture ; Coculture Techniques ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Life Sciences ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; Microbial Genetics and Genomics ; Microbiology ; Microorganisms ; Productivity ; Rhodobacteraceae - metabolism ; Sorbose - metabolism ; Stenotrophomonas maltophilia - metabolism ; Studies ; Sugar Acids - metabolism ; Yeast</subject><ispartof>Applied microbiology and biotechnology, 2010-03, Vol.86 (2), p.469-480</ispartof><rights>Springer-Verlag 2009</rights><rights>2015 INIST-CNRS</rights><rights>Springer-Verlag 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-9baca47cad3bfa697e67197ebc97aef050183a40449ace92f0589d5c4dbe30db3</citedby><cites>FETCH-LOGICAL-c461t-9baca47cad3bfa697e67197ebc97aef050183a40449ace92f0589d5c4dbe30db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-009-2312-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-009-2312-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22476801$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19902207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takagi, Yoshinori</creatorcontrib><creatorcontrib>Sugisawa, Teruhide</creatorcontrib><creatorcontrib>Hoshino, Tatsuo</creatorcontrib><title>Continuous 2-Keto-l-gulonic acid fermentation by mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium or Xanthomonas maltophilia</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>The fermentation process of 2-keto-L-gulonic acid (2KGA) from L-sorbose was developed using a two-stage continuous fermentation system. The mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium DSM 4026 produced 90 g/L of 2KGA from 120 g/L of L-sorbose at the dilution rate of 0.01 h⁻¹ in a single-stage continuous fermentation process. But after the production period was beyond 150 h, the significant decrease of 2KGA productivity was observed. When the non-spore forming bacteria Xanthomonas maltophilia IFO 12692 was used instead of B. megaterium DSM 4026 as a partner strain for K. vulgare DSM 4025, the 2KGA productivity was significantly improved in a two-stage continuous culture mode, in which two fermentors of the same size and volume were connected in series. In this mode, with two sets of 3-L jar fermentors, the steady state could be continued to over 1,331.5 h at least, when the dilution rates were 0.0382 h⁻¹ and 0.0380 hour⁻¹, respectively, for the first and second fermentors. The overall productivity was calculated to be 2.15 g/L/h at 113.1 g/L and a molar conversion yield of 90.1%. In the up-scaling fermentation to 30-L jar fermentors, 118.5 g/L of 2KGA was produced when dilution rates in both stages were 0.0430 hour⁻¹, and the overall productivity was calculated to be 2.55 g/L/h.</description><subject>Acids</subject><subject>Bacillus megaterium - metabolism</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnological Products and Process Engineering</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Coculture Techniques</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Life Sciences</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Productivity</subject><subject>Rhodobacteraceae - metabolism</subject><subject>Sorbose - metabolism</subject><subject>Stenotrophomonas maltophilia - metabolism</subject><subject>Studies</subject><subject>Sugar Acids - metabolism</subject><subject>Yeast</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc-KFDEQxoMo7jj6AF40COIpWklnOt1HHf_iiod1wVuoTqd7s6STMekW9018XNPM4IIHLylI_b6vivoIeczhJQdQrzKA2FUMoGWi4oLxO2TDZSUY1FzeJRvgasfUrm3OyIOcrwG4aOr6PjnjbQtCgNqQ3_sYZheWuGQq2Gc7R-bZuPgYnKFoXE8HmyYbZpxdDLS7oZP7ZXtqFj8vydI40FV0UrjRBrdM9OfiRyzdtxdfqCw7Ugw9fVPsvC9zJjvibNMKxkS_Y5iv4hQDlg76OR6unHf4kNwb0Gf76FS35PL9u2_7j-z864dP-9fnzMiaz6zt0KBUBvuqG7Bula0VL29nWoV2gB3wpkIJUrZobCvKT9P2OyP7zlbQd9WWvDj6HlL8sdg868llY73HYMtRtKqqukiUKuSzf8jruKRQltOiaSTUTWG3hB8hk2LOyQ76kNyE6UZz0Gto-hiaLqHpNTTNi-bJyXjpJtvfKk4pFeD5CcBs0A8Jg3H5LyeEVHUDq5E4crm0wmjT7Yb_m_70KBowahxTMb68EMWtXA5qELz6A5xkuhc</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Takagi, Yoshinori</creator><creator>Sugisawa, Teruhide</creator><creator>Hoshino, Tatsuo</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20100301</creationdate><title>Continuous 2-Keto-l-gulonic acid fermentation by mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium or Xanthomonas maltophilia</title><author>Takagi, Yoshinori ; Sugisawa, Teruhide ; Hoshino, Tatsuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-9baca47cad3bfa697e67197ebc97aef050183a40449ace92f0589d5c4dbe30db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acids</topic><topic>Bacillus megaterium - metabolism</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnological Products and Process Engineering</topic><topic>Biotechnology</topic><topic>Cell culture</topic><topic>Coculture Techniques</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Life Sciences</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial engineering. Fermentation and microbial culture technology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Productivity</topic><topic>Rhodobacteraceae - metabolism</topic><topic>Sorbose - metabolism</topic><topic>Stenotrophomonas maltophilia - metabolism</topic><topic>Studies</topic><topic>Sugar Acids - metabolism</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takagi, Yoshinori</creatorcontrib><creatorcontrib>Sugisawa, Teruhide</creatorcontrib><creatorcontrib>Hoshino, Tatsuo</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takagi, Yoshinori</au><au>Sugisawa, Teruhide</au><au>Hoshino, Tatsuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Continuous 2-Keto-l-gulonic acid fermentation by mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium or Xanthomonas maltophilia</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2010-03-01</date><risdate>2010</risdate><volume>86</volume><issue>2</issue><spage>469</spage><epage>480</epage><pages>469-480</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>The fermentation process of 2-keto-L-gulonic acid (2KGA) from L-sorbose was developed using a two-stage continuous fermentation system. The mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium DSM 4026 produced 90 g/L of 2KGA from 120 g/L of L-sorbose at the dilution rate of 0.01 h⁻¹ in a single-stage continuous fermentation process. But after the production period was beyond 150 h, the significant decrease of 2KGA productivity was observed. When the non-spore forming bacteria Xanthomonas maltophilia IFO 12692 was used instead of B. megaterium DSM 4026 as a partner strain for K. vulgare DSM 4025, the 2KGA productivity was significantly improved in a two-stage continuous culture mode, in which two fermentors of the same size and volume were connected in series. In this mode, with two sets of 3-L jar fermentors, the steady state could be continued to over 1,331.5 h at least, when the dilution rates were 0.0382 h⁻¹ and 0.0380 hour⁻¹, respectively, for the first and second fermentors. The overall productivity was calculated to be 2.15 g/L/h at 113.1 g/L and a molar conversion yield of 90.1%. In the up-scaling fermentation to 30-L jar fermentors, 118.5 g/L of 2KGA was produced when dilution rates in both stages were 0.0430 hour⁻¹, and the overall productivity was calculated to be 2.55 g/L/h.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>19902207</pmid><doi>10.1007/s00253-009-2312-1</doi><tpages>12</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0175-7598 |
ispartof | Applied microbiology and biotechnology, 2010-03, Vol.86 (2), p.469-480 |
issn | 0175-7598 1432-0614 |
language | eng |
recordid | cdi_proquest_miscellaneous_733658977 |
source | MEDLINE; Springer Nature - Complete Springer Journals |
subjects | Acids Bacillus megaterium - metabolism Bacteria Biological and medical sciences Biomedical and Life Sciences Biotechnological Products and Process Engineering Biotechnology Cell culture Coculture Techniques Fermentation Fundamental and applied biological sciences. Psychology Life Sciences Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Microbial Genetics and Genomics Microbiology Microorganisms Productivity Rhodobacteraceae - metabolism Sorbose - metabolism Stenotrophomonas maltophilia - metabolism Studies Sugar Acids - metabolism Yeast |
title | Continuous 2-Keto-l-gulonic acid fermentation by mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium or Xanthomonas maltophilia |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T06%3A24%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Continuous%202-Keto-l-gulonic%20acid%20fermentation%20by%20mixed%20culture%20of%20Ketogulonicigenium%20vulgare%20DSM%204025%20and%20Bacillus%20megaterium%20or%20Xanthomonas%20maltophilia&rft.jtitle=Applied%20microbiology%20and%20biotechnology&rft.au=Takagi,%20Yoshinori&rft.date=2010-03-01&rft.volume=86&rft.issue=2&rft.spage=469&rft.epage=480&rft.pages=469-480&rft.issn=0175-7598&rft.eissn=1432-0614&rft.coden=AMBIDG&rft_id=info:doi/10.1007/s00253-009-2312-1&rft_dat=%3Cproquest_cross%3E2036717741%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=288406873&rft_id=info:pmid/19902207&rfr_iscdi=true |