Interferon α–Induced Inhibition of Infectious Bursal Disease Virus in Chicken Embryo Fibroblast Cultures Differing in Mx Genotype
Interferon (IFN)-induced antiviral activity in cells forms an important early line of defense against viral pathogens. IFN-induced mediators are well documented in mammals, with one of the best-characterized antiviral proteins being Mx. In chickens, many alleles of Mx have been described, but functi...
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| Veröffentlicht in: | Avian diseases 2010-06, Vol.54 (2), p.802-806 |
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| creator | O'Neill, Ann Marie Livant, Emily J Ewald, Sandra J |
| description | Interferon (IFN)-induced antiviral activity in cells forms an important early line of defense against viral pathogens. IFN-induced mediators are well documented in mammals, with one of the best-characterized antiviral proteins being Mx. In chickens, many alleles of Mx have been described, but functionally only the polymorphism at a site encoding residue 631 in the protein determines differential antiviral activity against vesicular stomatitis virus and influenza virus in transfection experiments. The role of chicken Mx has not been assessed with regard to infectious bursal disease virus (IBDV), an important pathogen of chickens. To examine the role of chicken IFNα and the Mx631 single-nucleotide polymorphism against IBDV, chicken embryo fibroblast cultures (CEF) that differed in Mx genotype (antivirally positive Mx Asn631 or antivirally negative Mx Ser631) were treated with IFNα and viral yield was assessed following infection with D78 vaccine-strain IBDV. IFNα was shown to have strong antiviral activity in this system in terms of reduced virus yield. Furthermore, the reduction in viral yield did not differ significantly among Mx genotypes, indicating that Mx Asn631 is not a pivotal determinant of resistance to this strain of IBDV in CEF. |
| doi_str_mv | 10.1637/9001-072309-Reg.1 |
| format | Article |
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IFN-induced mediators are well documented in mammals, with one of the best-characterized antiviral proteins being Mx. In chickens, many alleles of Mx have been described, but functionally only the polymorphism at a site encoding residue 631 in the protein determines differential antiviral activity against vesicular stomatitis virus and influenza virus in transfection experiments. The role of chicken Mx has not been assessed with regard to infectious bursal disease virus (IBDV), an important pathogen of chickens. To examine the role of chicken IFNα and the Mx631 single-nucleotide polymorphism against IBDV, chicken embryo fibroblast cultures (CEF) that differed in Mx genotype (antivirally positive Mx Asn631 or antivirally negative Mx Ser631) were treated with IFNα and viral yield was assessed following infection with D78 vaccine-strain IBDV. IFNα was shown to have strong antiviral activity in this system in terms of reduced virus yield. Furthermore, the reduction in viral yield did not differ significantly among Mx genotypes, indicating that Mx Asn631 is not a pivotal determinant of resistance to this strain of IBDV in CEF.</description><identifier>ISSN: 0005-2086</identifier><identifier>EISSN: 1938-4351</identifier><identifier>DOI: 10.1637/9001-072309-Reg.1</identifier><identifier>PMID: 20608522</identifier><language>eng</language><publisher>United States: American Association of Avian Pathologists</publisher><subject>alleles ; Animals ; Antiviral Agents - pharmacology ; Antivirals ; Chick Embryo ; chicken ; Chickens ; cultured cells ; Double stranded RNA ; embryo (animal) ; embryo culture ; Embryonic cells ; Embryos ; fibroblasts ; Fibroblasts - metabolism ; Fibroblasts - virology ; food animals ; Gene Expression Regulation - immunology ; genetic polymorphism ; Genotype ; Genotypes ; GTP-Binding Proteins - genetics ; GTP-Binding Proteins - metabolism ; Infections ; Infectious bursal disease virus ; Infectious bursal disease virus - drug effects ; Infectious bursal disease virus - physiology ; interferon ; interferon-alpha ; Interferon-alpha - pharmacology ; interferons ; Mx ; Myxovirus Resistance Proteins ; Orthomyxoviridae ; Polymorphism, Single Nucleotide ; Regular s ; resistance mechanisms ; single nucleotide polymorphism ; vertebrate viruses ; Virus Replication ; Viruses</subject><ispartof>Avian diseases, 2010-06, Vol.54 (2), p.802-806</ispartof><rights>American Association of Avian Pathologists</rights><rights>Copyright 2010 American Association of Avian Pathologists, inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b386t-c7e5a6328387a0645277a8f603b2625781e0c0771e0136d32cb829b96dbdde013</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1637/9001-072309-Reg.1$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/40801703$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,26978,27924,27925,52363,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20608522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>O'Neill, Ann Marie</creatorcontrib><creatorcontrib>Livant, Emily J</creatorcontrib><creatorcontrib>Ewald, Sandra J</creatorcontrib><title>Interferon α–Induced Inhibition of Infectious Bursal Disease Virus in Chicken Embryo Fibroblast Cultures Differing in Mx Genotype</title><title>Avian diseases</title><addtitle>Avian Dis</addtitle><description>Interferon (IFN)-induced antiviral activity in cells forms an important early line of defense against viral pathogens. IFN-induced mediators are well documented in mammals, with one of the best-characterized antiviral proteins being Mx. In chickens, many alleles of Mx have been described, but functionally only the polymorphism at a site encoding residue 631 in the protein determines differential antiviral activity against vesicular stomatitis virus and influenza virus in transfection experiments. The role of chicken Mx has not been assessed with regard to infectious bursal disease virus (IBDV), an important pathogen of chickens. To examine the role of chicken IFNα and the Mx631 single-nucleotide polymorphism against IBDV, chicken embryo fibroblast cultures (CEF) that differed in Mx genotype (antivirally positive Mx Asn631 or antivirally negative Mx Ser631) were treated with IFNα and viral yield was assessed following infection with D78 vaccine-strain IBDV. IFNα was shown to have strong antiviral activity in this system in terms of reduced virus yield. Furthermore, the reduction in viral yield did not differ significantly among Mx genotypes, indicating that Mx Asn631 is not a pivotal determinant of resistance to this strain of IBDV in CEF.</description><subject>alleles</subject><subject>Animals</subject><subject>Antiviral Agents - pharmacology</subject><subject>Antivirals</subject><subject>Chick Embryo</subject><subject>chicken</subject><subject>Chickens</subject><subject>cultured cells</subject><subject>Double stranded RNA</subject><subject>embryo (animal)</subject><subject>embryo culture</subject><subject>Embryonic cells</subject><subject>Embryos</subject><subject>fibroblasts</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - virology</subject><subject>food animals</subject><subject>Gene Expression Regulation - immunology</subject><subject>genetic polymorphism</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>GTP-Binding Proteins - genetics</subject><subject>GTP-Binding Proteins - metabolism</subject><subject>Infections</subject><subject>Infectious bursal disease virus</subject><subject>Infectious bursal disease virus - drug effects</subject><subject>Infectious bursal disease virus - physiology</subject><subject>interferon</subject><subject>interferon-alpha</subject><subject>Interferon-alpha - pharmacology</subject><subject>interferons</subject><subject>Mx</subject><subject>Myxovirus Resistance Proteins</subject><subject>Orthomyxoviridae</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Regular s</subject><subject>resistance mechanisms</subject><subject>single nucleotide polymorphism</subject><subject>vertebrate viruses</subject><subject>Virus Replication</subject><subject>Viruses</subject><issn>0005-2086</issn><issn>1938-4351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQxi0EotuFB-AA-NZT2rGd2M4RlrasVIQElKtlJ5OtSzZe7ERibxx4A56EF-EheBK8SumZ02jm-80ffybkGYNTJoU6qwFYAYoLqIsPuDllD8iC1UIXpajYQ7IAgKrgoOUROU7pNtOqlvCYHHGQoCvOF-THehgxdhjDQH__-vP953popwZbuh5uvPOjz_XQ5azDJidToq-nmGxP3_iENiH97GMu-oGubnzzBQd6vnVxH-iFdzG43qaRrqZ-nCKm3NPlTX7YHPh33-glDmHc7_AJedTZPuHTu7gk1xfnn1Zvi6v3l-vVq6vCCS3HolFYWSm4FlpZkGXFlbK6kyAcl7xSmiE0oFQOTMhW8MZpXrtatq5tD7UlOZnn7mL4OmEazdanBvveDpifZpQQslS6LDPJZrKJIaWIndlFv7VxbxiYg_fm4L2ZvTfZe3OY_uJu-uS22N53_DM7A89n4DaNId7rJej8MSCy_nLWOxuM3USfzPVHnu8GpiVjee2SnM2E8yEM-B9H_QVGIKM7</recordid><startdate>20100601</startdate><enddate>20100601</enddate><creator>O'Neill, Ann Marie</creator><creator>Livant, Emily J</creator><creator>Ewald, Sandra J</creator><general>American Association of Avian Pathologists</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100601</creationdate><title>Interferon α–Induced Inhibition of Infectious Bursal Disease Virus in Chicken Embryo Fibroblast Cultures Differing in Mx Genotype</title><author>O'Neill, Ann Marie ; Livant, Emily J ; Ewald, Sandra J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b386t-c7e5a6328387a0645277a8f603b2625781e0c0771e0136d32cb829b96dbdde013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>alleles</topic><topic>Animals</topic><topic>Antiviral Agents - pharmacology</topic><topic>Antivirals</topic><topic>Chick Embryo</topic><topic>chicken</topic><topic>Chickens</topic><topic>cultured cells</topic><topic>Double stranded RNA</topic><topic>embryo (animal)</topic><topic>embryo culture</topic><topic>Embryonic cells</topic><topic>Embryos</topic><topic>fibroblasts</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - virology</topic><topic>food animals</topic><topic>Gene Expression Regulation - immunology</topic><topic>genetic polymorphism</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>GTP-Binding Proteins - genetics</topic><topic>GTP-Binding Proteins - metabolism</topic><topic>Infections</topic><topic>Infectious bursal disease virus</topic><topic>Infectious bursal disease virus - drug effects</topic><topic>Infectious bursal disease virus - physiology</topic><topic>interferon</topic><topic>interferon-alpha</topic><topic>Interferon-alpha - pharmacology</topic><topic>interferons</topic><topic>Mx</topic><topic>Myxovirus Resistance Proteins</topic><topic>Orthomyxoviridae</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Regular s</topic><topic>resistance mechanisms</topic><topic>single nucleotide polymorphism</topic><topic>vertebrate viruses</topic><topic>Virus Replication</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>O'Neill, Ann Marie</creatorcontrib><creatorcontrib>Livant, Emily J</creatorcontrib><creatorcontrib>Ewald, Sandra J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Avian diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>O'Neill, Ann Marie</au><au>Livant, Emily J</au><au>Ewald, Sandra J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interferon α–Induced Inhibition of Infectious Bursal Disease Virus in Chicken Embryo Fibroblast Cultures Differing in Mx Genotype</atitle><jtitle>Avian diseases</jtitle><addtitle>Avian Dis</addtitle><date>2010-06-01</date><risdate>2010</risdate><volume>54</volume><issue>2</issue><spage>802</spage><epage>806</epage><pages>802-806</pages><issn>0005-2086</issn><eissn>1938-4351</eissn><abstract>Interferon (IFN)-induced antiviral activity in cells forms an important early line of defense against viral pathogens. IFN-induced mediators are well documented in mammals, with one of the best-characterized antiviral proteins being Mx. In chickens, many alleles of Mx have been described, but functionally only the polymorphism at a site encoding residue 631 in the protein determines differential antiviral activity against vesicular stomatitis virus and influenza virus in transfection experiments. The role of chicken Mx has not been assessed with regard to infectious bursal disease virus (IBDV), an important pathogen of chickens. To examine the role of chicken IFNα and the Mx631 single-nucleotide polymorphism against IBDV, chicken embryo fibroblast cultures (CEF) that differed in Mx genotype (antivirally positive Mx Asn631 or antivirally negative Mx Ser631) were treated with IFNα and viral yield was assessed following infection with D78 vaccine-strain IBDV. IFNα was shown to have strong antiviral activity in this system in terms of reduced virus yield. Furthermore, the reduction in viral yield did not differ significantly among Mx genotypes, indicating that Mx Asn631 is not a pivotal determinant of resistance to this strain of IBDV in CEF.</abstract><cop>United States</cop><pub>American Association of Avian Pathologists</pub><pmid>20608522</pmid><doi>10.1637/9001-072309-Reg.1</doi><tpages>5</tpages></addata></record> |
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| subjects | alleles Animals Antiviral Agents - pharmacology Antivirals Chick Embryo chicken Chickens cultured cells Double stranded RNA embryo (animal) embryo culture Embryonic cells Embryos fibroblasts Fibroblasts - metabolism Fibroblasts - virology food animals Gene Expression Regulation - immunology genetic polymorphism Genotype Genotypes GTP-Binding Proteins - genetics GTP-Binding Proteins - metabolism Infections Infectious bursal disease virus Infectious bursal disease virus - drug effects Infectious bursal disease virus - physiology interferon interferon-alpha Interferon-alpha - pharmacology interferons Mx Myxovirus Resistance Proteins Orthomyxoviridae Polymorphism, Single Nucleotide Regular s resistance mechanisms single nucleotide polymorphism vertebrate viruses Virus Replication Viruses |
| title | Interferon α–Induced Inhibition of Infectious Bursal Disease Virus in Chicken Embryo Fibroblast Cultures Differing in Mx Genotype |
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