Omega-3 fatty acids modulate ATPases involved in duodenal Ca absorption
Dietary supplementation with fish oil that contains omega-3 polyunsaturated fatty acids has been shown to enhance bone density as well as duodenal calcium uptake in rats. The latter process is supported by membrane ATPases. The present in vitro study was undertaken to test the effect of omega-3 fatt...
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| Veröffentlicht in: | Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2003-06, Vol.68 (6), p.423-429 |
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| container_title | Prostaglandins, leukotrienes and essential fatty acids |
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| creator | Haag, Marianne Magada, Olga N Claassen, Nico Böhmer, Linde H Kruger, Marlena C |
| description | Dietary supplementation with fish oil that contains omega-3 polyunsaturated fatty acids has been shown to enhance bone density as well as duodenal calcium uptake in rats. The latter process is supported by membrane ATPases. The present in vitro study was undertaken to test the effect of omega-3 fatty acids on ATPase activity in isolated basolateral membranes from rat duodenal enterocytes. Ca-ATPase in calmodulin-stripped membranes was activated in a biphasic manner by docosahexanoic acid (DHA) (10–30
μg/ml) but not by eicosapentanoic acid (EPA). This effect was blocked partially by 0.5
μM calphostin (a protein kinase C blocker). DHA inhibited Na,K-ATPase (−49% of basal activity, [DHA]=30
μg/ml,
P |
| doi_str_mv | 10.1016/S0952-3278(03)00067-X |
| format | Article |
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μg/ml) but not by eicosapentanoic acid (EPA). This effect was blocked partially by 0.5
μM calphostin (a protein kinase C blocker). DHA inhibited Na,K-ATPase (−49% of basal activity, [DHA]=30
μg/ml,
P<0.01). This effect could be reversed partially by 50
μM genistein, a tyrosine kinase blocker. EPA also inhibited Na,K-ATPase: (−47% of basal activity, [EPA]=30
μg/ml,
P<0.01), this effect was partially reversed by 100
μM indomethacin, a cyclo-oxygenase blocker. Omega-3 fatty acids are thus involved in multiple signalling effects that effect ATPases in BLM.</description><identifier>ISSN: 0952-3278</identifier><identifier>EISSN: 1532-2823</identifier><identifier>DOI: 10.1016/S0952-3278(03)00067-X</identifier><identifier>PMID: 12798663</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>Absorption - drug effects ; Animals ; Apyrase - antagonists & inhibitors ; Apyrase - metabolism ; Ca absorption ; Ca-ATPase ; Calcium - metabolism ; Cell Membrane - drug effects ; Cell Membrane - enzymology ; Cellular signalling ; Docosahexanoic acid ; Dose-Response Relationship, Drug ; Duodenum ; Duodenum - cytology ; Duodenum - drug effects ; Duodenum - enzymology ; Duodenum - metabolism ; Eicosapentanoic acid ; Enterocytes - drug effects ; Enterocytes - enzymology ; Enterocytes - metabolism ; Enzyme Inhibitors - pharmacology ; Fatty Acids, Omega-3 - pharmacology ; Male ; Na,K-ATPase ; Naphthalenes - pharmacology ; Omega-3 fatty acids ; Polyunsaturated fatty acids ; Protein Kinase C - antagonists & inhibitors ; Protein Kinase C - metabolism ; Rats</subject><ispartof>Prostaglandins, leukotrienes and essential fatty acids, 2003-06, Vol.68 (6), p.423-429</ispartof><rights>2003 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-4cc5cd1408dca3b4e63d06c9345ea78cbdcb48f0bd1ab3d16f97a66e1f0a006c3</citedby><cites>FETCH-LOGICAL-c413t-4cc5cd1408dca3b4e63d06c9345ea78cbdcb48f0bd1ab3d16f97a66e1f0a006c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0952-3278(03)00067-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12798663$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haag, Marianne</creatorcontrib><creatorcontrib>Magada, Olga N</creatorcontrib><creatorcontrib>Claassen, Nico</creatorcontrib><creatorcontrib>Böhmer, Linde H</creatorcontrib><creatorcontrib>Kruger, Marlena C</creatorcontrib><title>Omega-3 fatty acids modulate ATPases involved in duodenal Ca absorption</title><title>Prostaglandins, leukotrienes and essential fatty acids</title><addtitle>Prostaglandins Leukot Essent Fatty Acids</addtitle><description>Dietary supplementation with fish oil that contains omega-3 polyunsaturated fatty acids has been shown to enhance bone density as well as duodenal calcium uptake in rats. The latter process is supported by membrane ATPases. The present in vitro study was undertaken to test the effect of omega-3 fatty acids on ATPase activity in isolated basolateral membranes from rat duodenal enterocytes. Ca-ATPase in calmodulin-stripped membranes was activated in a biphasic manner by docosahexanoic acid (DHA) (10–30
μg/ml) but not by eicosapentanoic acid (EPA). This effect was blocked partially by 0.5
μM calphostin (a protein kinase C blocker). DHA inhibited Na,K-ATPase (−49% of basal activity, [DHA]=30
μg/ml,
P<0.01). This effect could be reversed partially by 50
μM genistein, a tyrosine kinase blocker. EPA also inhibited Na,K-ATPase: (−47% of basal activity, [EPA]=30
μg/ml,
P<0.01), this effect was partially reversed by 100
μM indomethacin, a cyclo-oxygenase blocker. Omega-3 fatty acids are thus involved in multiple signalling effects that effect ATPases in BLM.</description><subject>Absorption - drug effects</subject><subject>Animals</subject><subject>Apyrase - antagonists & inhibitors</subject><subject>Apyrase - metabolism</subject><subject>Ca absorption</subject><subject>Ca-ATPase</subject><subject>Calcium - metabolism</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - enzymology</subject><subject>Cellular signalling</subject><subject>Docosahexanoic acid</subject><subject>Dose-Response Relationship, Drug</subject><subject>Duodenum</subject><subject>Duodenum - cytology</subject><subject>Duodenum - drug effects</subject><subject>Duodenum - enzymology</subject><subject>Duodenum - metabolism</subject><subject>Eicosapentanoic acid</subject><subject>Enterocytes - drug effects</subject><subject>Enterocytes - enzymology</subject><subject>Enterocytes - metabolism</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fatty Acids, Omega-3 - pharmacology</subject><subject>Male</subject><subject>Na,K-ATPase</subject><subject>Naphthalenes - pharmacology</subject><subject>Omega-3 fatty acids</subject><subject>Polyunsaturated fatty acids</subject><subject>Protein Kinase C - antagonists & inhibitors</subject><subject>Protein Kinase C - metabolism</subject><subject>Rats</subject><issn>0952-3278</issn><issn>1532-2823</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo7vrxE5ScRA_VJNOm7Ulk0VVYWMEV9hbSZCqRtlmbdsF_b_cDPXqaOTzvvMxDyAVnt5xxeffG8kREINLsmsENY0ym0fKAjHkCIhKZgEMy_kVG5CSEzwESnMfHZMRFmmdSwphM5zV-6Ahoqbvum2rjbKC1t32lO6QPi1cdMFDXrH21Rjss1PbeYqMrOtFUF8G3q8755owclboKeL6fp-T96XExeY5m8-nL5GEWmZhDF8XGJMbymGXWaChilGCZNDnECeo0M4U1RZyVrLBcF2C5LPNUS4m8ZHp40cApudrdXbX-q8fQqdoFg1WlG_R9UCmAhDwVA5jsQNP6EFos1ap1tW6_FWdqY1BtDaqNHsVAbQ2q5ZC73Bf0RY32L7VXNgD3OwCHN9cOWxWMw8agdS2aTlnv_qn4AUzOgL4</recordid><startdate>20030601</startdate><enddate>20030601</enddate><creator>Haag, Marianne</creator><creator>Magada, Olga N</creator><creator>Claassen, Nico</creator><creator>Böhmer, Linde H</creator><creator>Kruger, Marlena C</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030601</creationdate><title>Omega-3 fatty acids modulate ATPases involved in duodenal Ca absorption</title><author>Haag, Marianne ; Magada, Olga N ; Claassen, Nico ; Böhmer, Linde H ; Kruger, Marlena C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-4cc5cd1408dca3b4e63d06c9345ea78cbdcb48f0bd1ab3d16f97a66e1f0a006c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Absorption - drug effects</topic><topic>Animals</topic><topic>Apyrase - antagonists & inhibitors</topic><topic>Apyrase - metabolism</topic><topic>Ca absorption</topic><topic>Ca-ATPase</topic><topic>Calcium - metabolism</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - enzymology</topic><topic>Cellular signalling</topic><topic>Docosahexanoic acid</topic><topic>Dose-Response Relationship, Drug</topic><topic>Duodenum</topic><topic>Duodenum - cytology</topic><topic>Duodenum - drug effects</topic><topic>Duodenum - enzymology</topic><topic>Duodenum - metabolism</topic><topic>Eicosapentanoic acid</topic><topic>Enterocytes - drug effects</topic><topic>Enterocytes - enzymology</topic><topic>Enterocytes - metabolism</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fatty Acids, Omega-3 - pharmacology</topic><topic>Male</topic><topic>Na,K-ATPase</topic><topic>Naphthalenes - pharmacology</topic><topic>Omega-3 fatty acids</topic><topic>Polyunsaturated fatty acids</topic><topic>Protein Kinase C - antagonists & inhibitors</topic><topic>Protein Kinase C - metabolism</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haag, Marianne</creatorcontrib><creatorcontrib>Magada, Olga N</creatorcontrib><creatorcontrib>Claassen, Nico</creatorcontrib><creatorcontrib>Böhmer, Linde H</creatorcontrib><creatorcontrib>Kruger, Marlena C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Prostaglandins, leukotrienes and essential fatty acids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haag, Marianne</au><au>Magada, Olga N</au><au>Claassen, Nico</au><au>Böhmer, Linde H</au><au>Kruger, Marlena C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Omega-3 fatty acids modulate ATPases involved in duodenal Ca absorption</atitle><jtitle>Prostaglandins, leukotrienes and essential fatty acids</jtitle><addtitle>Prostaglandins Leukot Essent Fatty Acids</addtitle><date>2003-06-01</date><risdate>2003</risdate><volume>68</volume><issue>6</issue><spage>423</spage><epage>429</epage><pages>423-429</pages><issn>0952-3278</issn><eissn>1532-2823</eissn><abstract>Dietary supplementation with fish oil that contains omega-3 polyunsaturated fatty acids has been shown to enhance bone density as well as duodenal calcium uptake in rats. The latter process is supported by membrane ATPases. The present in vitro study was undertaken to test the effect of omega-3 fatty acids on ATPase activity in isolated basolateral membranes from rat duodenal enterocytes. Ca-ATPase in calmodulin-stripped membranes was activated in a biphasic manner by docosahexanoic acid (DHA) (10–30
μg/ml) but not by eicosapentanoic acid (EPA). This effect was blocked partially by 0.5
μM calphostin (a protein kinase C blocker). DHA inhibited Na,K-ATPase (−49% of basal activity, [DHA]=30
μg/ml,
P<0.01). This effect could be reversed partially by 50
μM genistein, a tyrosine kinase blocker. EPA also inhibited Na,K-ATPase: (−47% of basal activity, [EPA]=30
μg/ml,
P<0.01), this effect was partially reversed by 100
μM indomethacin, a cyclo-oxygenase blocker. Omega-3 fatty acids are thus involved in multiple signalling effects that effect ATPases in BLM.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>12798663</pmid><doi>10.1016/S0952-3278(03)00067-X</doi><tpages>7</tpages></addata></record> |
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| subjects | Absorption - drug effects Animals Apyrase - antagonists & inhibitors Apyrase - metabolism Ca absorption Ca-ATPase Calcium - metabolism Cell Membrane - drug effects Cell Membrane - enzymology Cellular signalling Docosahexanoic acid Dose-Response Relationship, Drug Duodenum Duodenum - cytology Duodenum - drug effects Duodenum - enzymology Duodenum - metabolism Eicosapentanoic acid Enterocytes - drug effects Enterocytes - enzymology Enterocytes - metabolism Enzyme Inhibitors - pharmacology Fatty Acids, Omega-3 - pharmacology Male Na,K-ATPase Naphthalenes - pharmacology Omega-3 fatty acids Polyunsaturated fatty acids Protein Kinase C - antagonists & inhibitors Protein Kinase C - metabolism Rats |
| title | Omega-3 fatty acids modulate ATPases involved in duodenal Ca absorption |
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