Detection of Kinase Activity Using Versatile Fluorescence Quencher Probes
Turn off the light! A kinase assay system employs fluorescent peptides and a phosphate‐selective fluorescence quencher probe, and can be placed on a microfluidic chip (see picture). The system can be used for real‐time kinase monitoring, kinase inhibitor screening, and cancer diagnosis based on abno...
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| Veröffentlicht in: | Angewandte Chemie (International ed.) 2010-07, Vol.49 (29), p.4919-4923 |
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| container_end_page | 4923 |
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| container_issue | 29 |
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| container_title | Angewandte Chemie (International ed.) |
| container_volume | 49 |
| creator | Rhee, Hyun-Woo Lee, Seung Hwan Shin, Ik-Soo Choi, So Jung Park, Hun Hee Han, Kyungja Park, Tai Hyun Hong, Jong-In |
| description | Turn off the light! A kinase assay system employs fluorescent peptides and a phosphate‐selective fluorescence quencher probe, and can be placed on a microfluidic chip (see picture). The system can be used for real‐time kinase monitoring, kinase inhibitor screening, and cancer diagnosis based on abnormal kinase activity observed in patients' samples. |
| doi_str_mv | 10.1002/anie.201000879 |
| format | Article |
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A kinase assay system employs fluorescent peptides and a phosphate‐selective fluorescence quencher probe, and can be placed on a microfluidic chip (see picture). 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A kinase assay system employs fluorescent peptides and a phosphate‐selective fluorescence quencher probe, and can be placed on a microfluidic chip (see picture). 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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism cancer Casein Kinase II - metabolism Cyclic AMP-Dependent Protein Kinases - metabolism fluorescence Fluorescent Dyes - chemistry fluorescent probes Heterocyclic Compounds, 3-Ring - chemistry Humans kinases Microfluidic Analytical Techniques - methods Neoplasms - diagnosis Neoplasms - enzymology Phosphorylation Protein Kinase Inhibitors - chemistry Protein Kinase Inhibitors - pharmacology Protein Kinases - chemistry Protein Kinases - metabolism Proto-Oncogene Proteins c-abl - metabolism |
| title | Detection of Kinase Activity Using Versatile Fluorescence Quencher Probes |
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