Accurate analysis of taurine, anserine, carnosine and free amino acids in a cattle muscle biopsy sample
ABSTRACT We have established an analysis method for some free amino acids (FAAs), as well as taurine (Tau), anserine (Ans) and carnosine (Car), in a fresh biopsy sample from cattle muscle. A series of model biopsy samples, corresponding to the mixtures of lean meat, fat and connective tissue, was pr...
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| Veröffentlicht in: | Animal science journal 2010-06, Vol.81 (3), p.369-376 |
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| creator | IMANARI, Mai HIGUCHI, Mikito SHIBA, Nobuya WATANABE, Akira |
| description | ABSTRACT
We have established an analysis method for some free amino acids (FAAs), as well as taurine (Tau), anserine (Ans) and carnosine (Car), in a fresh biopsy sample from cattle muscle. A series of model biopsy samples, corresponding to the mixtures of lean meat, fat and connective tissue, was prepared and showed high correlation coefficients between the compound concentration and the 3‐methylhistidine (3‐MeHis) content derived from hydrolysis of the biopsy sample (r = 0.74–0.95, P |
| doi_str_mv | 10.1111/j.1740-0929.2010.00751.x |
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We have established an analysis method for some free amino acids (FAAs), as well as taurine (Tau), anserine (Ans) and carnosine (Car), in a fresh biopsy sample from cattle muscle. A series of model biopsy samples, corresponding to the mixtures of lean meat, fat and connective tissue, was prepared and showed high correlation coefficients between the compound concentration and the 3‐methylhistidine (3‐MeHis) content derived from hydrolysis of the biopsy sample (r = 0.74–0.95, P < 0.01). Interference from blood contamination could not be neglected, because the concentration of some FAAs in blood was comparable to that in muscle. However, it was possible to control the contamination of Tau, Ans, Car, glutamic acid, glutamine, asparatic acid and alanine to less than 5.0% when the blood contamination was controlled to less than 23%.These results suggest the necessity of measuring 3‐MeHis as an index of lean meat and hemoglobin as an index of blood contamination when compounds in muscle biopsy samples are evaluated. We have carried out a series of these analyses using one biopsy sample and reveal differences in Tau, Ans, Car and some FAAs in beef muscle after different feeding regimes.</description><identifier>ISSN: 1344-3941</identifier><identifier>EISSN: 1740-0929</identifier><identifier>DOI: 10.1111/j.1740-0929.2010.00751.x</identifier><identifier>PMID: 20597895</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Amino acids ; Amino Acids - analysis ; Animals ; Anserine - analysis ; Biopsy ; Biopsy - veterinary ; Carnosine - analysis ; Cattle ; Cattle - metabolism ; feeding ; free amino acids ; muscle ; Muscle, Skeletal - chemistry ; Muscular system ; Research methodology ; Taurine - analysis</subject><ispartof>Animal science journal, 2010-06, Vol.81 (3), p.369-376</ispartof><rights>2010 The Authors. Journal compilation © 2010 Japanese Society of Animal Science</rights><rights>Journal compilation © 2010 Japanese Society of Animal Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5511-97258228899926a207b03fbb36fd8df8b3176bc3e0e720f222375827ddb23fae3</citedby><cites>FETCH-LOGICAL-c5511-97258228899926a207b03fbb36fd8df8b3176bc3e0e720f222375827ddb23fae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1740-0929.2010.00751.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1740-0929.2010.00751.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20597895$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>IMANARI, Mai</creatorcontrib><creatorcontrib>HIGUCHI, Mikito</creatorcontrib><creatorcontrib>SHIBA, Nobuya</creatorcontrib><creatorcontrib>WATANABE, Akira</creatorcontrib><title>Accurate analysis of taurine, anserine, carnosine and free amino acids in a cattle muscle biopsy sample</title><title>Animal science journal</title><addtitle>Anim Sci J</addtitle><description>ABSTRACT
We have established an analysis method for some free amino acids (FAAs), as well as taurine (Tau), anserine (Ans) and carnosine (Car), in a fresh biopsy sample from cattle muscle. A series of model biopsy samples, corresponding to the mixtures of lean meat, fat and connective tissue, was prepared and showed high correlation coefficients between the compound concentration and the 3‐methylhistidine (3‐MeHis) content derived from hydrolysis of the biopsy sample (r = 0.74–0.95, P < 0.01). Interference from blood contamination could not be neglected, because the concentration of some FAAs in blood was comparable to that in muscle. However, it was possible to control the contamination of Tau, Ans, Car, glutamic acid, glutamine, asparatic acid and alanine to less than 5.0% when the blood contamination was controlled to less than 23%.These results suggest the necessity of measuring 3‐MeHis as an index of lean meat and hemoglobin as an index of blood contamination when compounds in muscle biopsy samples are evaluated. We have carried out a series of these analyses using one biopsy sample and reveal differences in Tau, Ans, Car and some FAAs in beef muscle after different feeding regimes.</description><subject>Amino acids</subject><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Anserine - analysis</subject><subject>Biopsy</subject><subject>Biopsy - veterinary</subject><subject>Carnosine - analysis</subject><subject>Cattle</subject><subject>Cattle - metabolism</subject><subject>feeding</subject><subject>free amino acids</subject><subject>muscle</subject><subject>Muscle, Skeletal - chemistry</subject><subject>Muscular system</subject><subject>Research methodology</subject><subject>Taurine - analysis</subject><issn>1344-3941</issn><issn>1740-0929</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi1ERUvhLyCLCxeyHdtxHEtcVitoQdXy_XGznMRGXvKxtRN19993til74IQv82r8vKPRO4RQBguG72KzYCqHDDTXCw7YBVCSLXaPyNnx4zFqkeeZ0Dk7JU9T2gAwpUE-IaccpFallmfk97Kup2hHR21v230KiQ6ejnaKoXevsZncrGob-yGhxF5DfXQoutAP1NahSTT01CIzjq2j3ZRqLFUYtmlPk-22rXtGTrxtk3v-UM_J93dvv62usuuPl-9Xy-uslpKxTCsuS87LUmvNC8tBVSB8VYnCN2Xjy0owVVS1cOAUB885FwoNqmkqLrx14py8mudu43AzuTSaLqTata3t3TAlo4QoBHDGkXz5D7kZpoghJCNkoXOAQiFUzlAdh5Si82YbQ2fj3jAwh1OYjTkkbg6Jm8MpzP0pzA6tLx7mT1XnmqPxb_YIvJmB29C6_X8PNsuvH1CgPZvtIY1ud7Tb-Mfg4kqan-tL82O9_iS__PpsVuIOjX-lfg</recordid><startdate>201006</startdate><enddate>201006</enddate><creator>IMANARI, Mai</creator><creator>HIGUCHI, Mikito</creator><creator>SHIBA, Nobuya</creator><creator>WATANABE, Akira</creator><general>Blackwell Publishing Asia</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201006</creationdate><title>Accurate analysis of taurine, anserine, carnosine and free amino acids in a cattle muscle biopsy sample</title><author>IMANARI, Mai ; HIGUCHI, Mikito ; SHIBA, Nobuya ; WATANABE, Akira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5511-97258228899926a207b03fbb36fd8df8b3176bc3e0e720f222375827ddb23fae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino acids</topic><topic>Amino Acids - analysis</topic><topic>Animals</topic><topic>Anserine - analysis</topic><topic>Biopsy</topic><topic>Biopsy - veterinary</topic><topic>Carnosine - analysis</topic><topic>Cattle</topic><topic>Cattle - metabolism</topic><topic>feeding</topic><topic>free amino acids</topic><topic>muscle</topic><topic>Muscle, Skeletal - chemistry</topic><topic>Muscular system</topic><topic>Research methodology</topic><topic>Taurine - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>IMANARI, Mai</creatorcontrib><creatorcontrib>HIGUCHI, Mikito</creatorcontrib><creatorcontrib>SHIBA, Nobuya</creatorcontrib><creatorcontrib>WATANABE, Akira</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Animal science journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>IMANARI, Mai</au><au>HIGUCHI, Mikito</au><au>SHIBA, Nobuya</au><au>WATANABE, Akira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accurate analysis of taurine, anserine, carnosine and free amino acids in a cattle muscle biopsy sample</atitle><jtitle>Animal science journal</jtitle><addtitle>Anim Sci J</addtitle><date>2010-06</date><risdate>2010</risdate><volume>81</volume><issue>3</issue><spage>369</spage><epage>376</epage><pages>369-376</pages><issn>1344-3941</issn><eissn>1740-0929</eissn><abstract>ABSTRACT
We have established an analysis method for some free amino acids (FAAs), as well as taurine (Tau), anserine (Ans) and carnosine (Car), in a fresh biopsy sample from cattle muscle. A series of model biopsy samples, corresponding to the mixtures of lean meat, fat and connective tissue, was prepared and showed high correlation coefficients between the compound concentration and the 3‐methylhistidine (3‐MeHis) content derived from hydrolysis of the biopsy sample (r = 0.74–0.95, P < 0.01). Interference from blood contamination could not be neglected, because the concentration of some FAAs in blood was comparable to that in muscle. However, it was possible to control the contamination of Tau, Ans, Car, glutamic acid, glutamine, asparatic acid and alanine to less than 5.0% when the blood contamination was controlled to less than 23%.These results suggest the necessity of measuring 3‐MeHis as an index of lean meat and hemoglobin as an index of blood contamination when compounds in muscle biopsy samples are evaluated. We have carried out a series of these analyses using one biopsy sample and reveal differences in Tau, Ans, Car and some FAAs in beef muscle after different feeding regimes.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>20597895</pmid><doi>10.1111/j.1740-0929.2010.00751.x</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Amino acids Amino Acids - analysis Animals Anserine - analysis Biopsy Biopsy - veterinary Carnosine - analysis Cattle Cattle - metabolism feeding free amino acids muscle Muscle, Skeletal - chemistry Muscular system Research methodology Taurine - analysis |
| title | Accurate analysis of taurine, anserine, carnosine and free amino acids in a cattle muscle biopsy sample |
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