AF1q enhancement of gamma irradiation-induced apoptosis by up-regulation of BAD expression via NF-kappaB in human squamous carcinoma A431 cells
BAD (BCL-2 antagonist of cell death) is a pro-apoptotic BCL-2 family protein that plays a critical role in the regulation of apoptotic response. This study presents direct evidence that AF1q increased the radiation-induced apoptosis through up-regulation of BAD in human squamous carcinoma A431 cells...
Gespeichert in:
| Veröffentlicht in: | Oncology reports 2010-08, Vol.24 (2), p.547-554 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 554 |
|---|---|
| container_issue | 2 |
| container_start_page | 547 |
| container_title | Oncology reports |
| container_volume | 24 |
| creator | Co, Ngai Na Tsang, Wing Pui Tsang, Tsun Yee Yeung, Chi Lam Au Yau, Pak Lun Kong, Siu Kai Kwok, Tim Tak |
| description | BAD (BCL-2 antagonist of cell death) is a pro-apoptotic BCL-2 family protein that plays a critical role in the regulation of apoptotic response. This study presents direct evidence that AF1q increased the radiation-induced apoptosis through up-regulation of BAD in human squamous carcinoma A431 cells and the key transcription factor involved is NF-kappaB. The minimal promoter sequence of BAD was identified; the activity was increased in AF1q stable transfectants and decreased upon AF1q siRNA transfection. The NF-kappaB consensus binding sequence is detected on BAD promoter. Inactivation of NF-kappaB by NF-kappaB inhibitor Bay 11-7082 or NF-kappaB p65 siRNA suppressed the expression and promoter activity of BAD; the suppression is more obvious in AF1q stable transfectants which also have an elevated NF-kappaB level. Mutation of putative NF-kappaB motif decreased the BAD promoter activity. The binding of NF-kappaB to the BAD promoter was confirmed by chromatin-immunoprecipitation. These findings indicate that AF1q up-regulation of BAD is through its effect on NF-kappaB and this may hint of its oncogenic mechanism in cancer. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_733628877</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>733628877</sourcerecordid><originalsourceid>FETCH-LOGICAL-p555-6e7dee8b75303095cb62215689659c4cac171e030fa41edb983440e9013af8b53</originalsourceid><addsrcrecordid>eNo1kMFOwzAMhiskxMbgFVBunCo1TZM0x24wQJrgsnvlpu4WaNKsaRF7Cl6ZDsbJkv3Z_vRfRHMqFY3TjNFZdB3Ce5KkMhHqKpqlCVdCZHwefRdreiDo9uA0WnQD6RqyA2uBmL6H2sBgOhcbV48aawK-80MXTCDVkYw-7nE3tr_IaW9ZPBD88j2GcOp8GiCv6_gDvIclMY7sRwuOhMMIthsD0dBr47rpVTE5Eo1tG26iywbagLfnuoi268ft6jnevD29rIpN7DnnsUBZI-aV5CxhieK6EmlKuciV4EpnGjSVFKdRAxnFulI5y7IEVUIZNHnF2SK6_zvr--4wYhhKa8JJABxOaqVkTKR5LuVE3p3JsbJYl743Fvpj-R8h-wGNPmzC</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733628877</pqid></control><display><type>article</type><title>AF1q enhancement of gamma irradiation-induced apoptosis by up-regulation of BAD expression via NF-kappaB in human squamous carcinoma A431 cells</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Co, Ngai Na ; Tsang, Wing Pui ; Tsang, Tsun Yee ; Yeung, Chi Lam Au ; Yau, Pak Lun ; Kong, Siu Kai ; Kwok, Tim Tak</creator><creatorcontrib>Co, Ngai Na ; Tsang, Wing Pui ; Tsang, Tsun Yee ; Yeung, Chi Lam Au ; Yau, Pak Lun ; Kong, Siu Kai ; Kwok, Tim Tak</creatorcontrib><description>BAD (BCL-2 antagonist of cell death) is a pro-apoptotic BCL-2 family protein that plays a critical role in the regulation of apoptotic response. This study presents direct evidence that AF1q increased the radiation-induced apoptosis through up-regulation of BAD in human squamous carcinoma A431 cells and the key transcription factor involved is NF-kappaB. The minimal promoter sequence of BAD was identified; the activity was increased in AF1q stable transfectants and decreased upon AF1q siRNA transfection. The NF-kappaB consensus binding sequence is detected on BAD promoter. Inactivation of NF-kappaB by NF-kappaB inhibitor Bay 11-7082 or NF-kappaB p65 siRNA suppressed the expression and promoter activity of BAD; the suppression is more obvious in AF1q stable transfectants which also have an elevated NF-kappaB level. Mutation of putative NF-kappaB motif decreased the BAD promoter activity. The binding of NF-kappaB to the BAD promoter was confirmed by chromatin-immunoprecipitation. These findings indicate that AF1q up-regulation of BAD is through its effect on NF-kappaB and this may hint of its oncogenic mechanism in cancer.</description><identifier>EISSN: 1791-2431</identifier><identifier>PMID: 20596645</identifier><language>eng</language><publisher>Greece</publisher><subject>Apoptosis - drug effects ; Apoptosis - genetics ; Apoptosis - radiation effects ; Base Sequence ; bcl-Associated Death Protein - genetics ; bcl-Associated Death Protein - metabolism ; Blood Proteins - antagonists & inhibitors ; Blood Proteins - genetics ; Blood Proteins - physiology ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - pathology ; Cell Line, Tumor ; Gamma Rays ; Gene Expression Regulation, Neoplastic - drug effects ; Gene Expression Regulation, Neoplastic - radiation effects ; Humans ; Molecular Sequence Data ; Neoplasm Proteins - antagonists & inhibitors ; Neoplasm Proteins - genetics ; Neoplasm Proteins - physiology ; NF-kappa B - antagonists & inhibitors ; NF-kappa B - genetics ; NF-kappa B - metabolism ; NF-kappa B - physiology ; Promoter Regions, Genetic ; Protein Binding ; Proto-Oncogene Proteins ; RNA, Small Interfering - pharmacology ; Transcription, Genetic - drug effects ; Transcription, Genetic - genetics ; Up-Regulation - drug effects ; Up-Regulation - genetics</subject><ispartof>Oncology reports, 2010-08, Vol.24 (2), p.547-554</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20596645$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Co, Ngai Na</creatorcontrib><creatorcontrib>Tsang, Wing Pui</creatorcontrib><creatorcontrib>Tsang, Tsun Yee</creatorcontrib><creatorcontrib>Yeung, Chi Lam Au</creatorcontrib><creatorcontrib>Yau, Pak Lun</creatorcontrib><creatorcontrib>Kong, Siu Kai</creatorcontrib><creatorcontrib>Kwok, Tim Tak</creatorcontrib><title>AF1q enhancement of gamma irradiation-induced apoptosis by up-regulation of BAD expression via NF-kappaB in human squamous carcinoma A431 cells</title><title>Oncology reports</title><addtitle>Oncol Rep</addtitle><description>BAD (BCL-2 antagonist of cell death) is a pro-apoptotic BCL-2 family protein that plays a critical role in the regulation of apoptotic response. This study presents direct evidence that AF1q increased the radiation-induced apoptosis through up-regulation of BAD in human squamous carcinoma A431 cells and the key transcription factor involved is NF-kappaB. The minimal promoter sequence of BAD was identified; the activity was increased in AF1q stable transfectants and decreased upon AF1q siRNA transfection. The NF-kappaB consensus binding sequence is detected on BAD promoter. Inactivation of NF-kappaB by NF-kappaB inhibitor Bay 11-7082 or NF-kappaB p65 siRNA suppressed the expression and promoter activity of BAD; the suppression is more obvious in AF1q stable transfectants which also have an elevated NF-kappaB level. Mutation of putative NF-kappaB motif decreased the BAD promoter activity. The binding of NF-kappaB to the BAD promoter was confirmed by chromatin-immunoprecipitation. These findings indicate that AF1q up-regulation of BAD is through its effect on NF-kappaB and this may hint of its oncogenic mechanism in cancer.</description><subject>Apoptosis - drug effects</subject><subject>Apoptosis - genetics</subject><subject>Apoptosis - radiation effects</subject><subject>Base Sequence</subject><subject>bcl-Associated Death Protein - genetics</subject><subject>bcl-Associated Death Protein - metabolism</subject><subject>Blood Proteins - antagonists & inhibitors</subject><subject>Blood Proteins - genetics</subject><subject>Blood Proteins - physiology</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Cell Line, Tumor</subject><subject>Gamma Rays</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Gene Expression Regulation, Neoplastic - radiation effects</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Neoplasm Proteins - antagonists & inhibitors</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Proteins - physiology</subject><subject>NF-kappa B - antagonists & inhibitors</subject><subject>NF-kappa B - genetics</subject><subject>NF-kappa B - metabolism</subject><subject>NF-kappa B - physiology</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Binding</subject><subject>Proto-Oncogene Proteins</subject><subject>RNA, Small Interfering - pharmacology</subject><subject>Transcription, Genetic - drug effects</subject><subject>Transcription, Genetic - genetics</subject><subject>Up-Regulation - drug effects</subject><subject>Up-Regulation - genetics</subject><issn>1791-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMFOwzAMhiskxMbgFVBunCo1TZM0x24wQJrgsnvlpu4WaNKsaRF7Cl6ZDsbJkv3Z_vRfRHMqFY3TjNFZdB3Ce5KkMhHqKpqlCVdCZHwefRdreiDo9uA0WnQD6RqyA2uBmL6H2sBgOhcbV48aawK-80MXTCDVkYw-7nE3tr_IaW9ZPBD88j2GcOp8GiCv6_gDvIclMY7sRwuOhMMIthsD0dBr47rpVTE5Eo1tG26iywbagLfnuoi268ft6jnevD29rIpN7DnnsUBZI-aV5CxhieK6EmlKuciV4EpnGjSVFKdRAxnFulI5y7IEVUIZNHnF2SK6_zvr--4wYhhKa8JJABxOaqVkTKR5LuVE3p3JsbJYl743Fvpj-R8h-wGNPmzC</recordid><startdate>201008</startdate><enddate>201008</enddate><creator>Co, Ngai Na</creator><creator>Tsang, Wing Pui</creator><creator>Tsang, Tsun Yee</creator><creator>Yeung, Chi Lam Au</creator><creator>Yau, Pak Lun</creator><creator>Kong, Siu Kai</creator><creator>Kwok, Tim Tak</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201008</creationdate><title>AF1q enhancement of gamma irradiation-induced apoptosis by up-regulation of BAD expression via NF-kappaB in human squamous carcinoma A431 cells</title><author>Co, Ngai Na ; Tsang, Wing Pui ; Tsang, Tsun Yee ; Yeung, Chi Lam Au ; Yau, Pak Lun ; Kong, Siu Kai ; Kwok, Tim Tak</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p555-6e7dee8b75303095cb62215689659c4cac171e030fa41edb983440e9013af8b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Apoptosis - drug effects</topic><topic>Apoptosis - genetics</topic><topic>Apoptosis - radiation effects</topic><topic>Base Sequence</topic><topic>bcl-Associated Death Protein - genetics</topic><topic>bcl-Associated Death Protein - metabolism</topic><topic>Blood Proteins - antagonists & inhibitors</topic><topic>Blood Proteins - genetics</topic><topic>Blood Proteins - physiology</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Cell Line, Tumor</topic><topic>Gamma Rays</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Gene Expression Regulation, Neoplastic - radiation effects</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Neoplasm Proteins - antagonists & inhibitors</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Proteins - physiology</topic><topic>NF-kappa B - antagonists & inhibitors</topic><topic>NF-kappa B - genetics</topic><topic>NF-kappa B - metabolism</topic><topic>NF-kappa B - physiology</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Binding</topic><topic>Proto-Oncogene Proteins</topic><topic>RNA, Small Interfering - pharmacology</topic><topic>Transcription, Genetic - drug effects</topic><topic>Transcription, Genetic - genetics</topic><topic>Up-Regulation - drug effects</topic><topic>Up-Regulation - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Co, Ngai Na</creatorcontrib><creatorcontrib>Tsang, Wing Pui</creatorcontrib><creatorcontrib>Tsang, Tsun Yee</creatorcontrib><creatorcontrib>Yeung, Chi Lam Au</creatorcontrib><creatorcontrib>Yau, Pak Lun</creatorcontrib><creatorcontrib>Kong, Siu Kai</creatorcontrib><creatorcontrib>Kwok, Tim Tak</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Oncology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Co, Ngai Na</au><au>Tsang, Wing Pui</au><au>Tsang, Tsun Yee</au><au>Yeung, Chi Lam Au</au><au>Yau, Pak Lun</au><au>Kong, Siu Kai</au><au>Kwok, Tim Tak</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AF1q enhancement of gamma irradiation-induced apoptosis by up-regulation of BAD expression via NF-kappaB in human squamous carcinoma A431 cells</atitle><jtitle>Oncology reports</jtitle><addtitle>Oncol Rep</addtitle><date>2010-08</date><risdate>2010</risdate><volume>24</volume><issue>2</issue><spage>547</spage><epage>554</epage><pages>547-554</pages><eissn>1791-2431</eissn><abstract>BAD (BCL-2 antagonist of cell death) is a pro-apoptotic BCL-2 family protein that plays a critical role in the regulation of apoptotic response. This study presents direct evidence that AF1q increased the radiation-induced apoptosis through up-regulation of BAD in human squamous carcinoma A431 cells and the key transcription factor involved is NF-kappaB. The minimal promoter sequence of BAD was identified; the activity was increased in AF1q stable transfectants and decreased upon AF1q siRNA transfection. The NF-kappaB consensus binding sequence is detected on BAD promoter. Inactivation of NF-kappaB by NF-kappaB inhibitor Bay 11-7082 or NF-kappaB p65 siRNA suppressed the expression and promoter activity of BAD; the suppression is more obvious in AF1q stable transfectants which also have an elevated NF-kappaB level. Mutation of putative NF-kappaB motif decreased the BAD promoter activity. The binding of NF-kappaB to the BAD promoter was confirmed by chromatin-immunoprecipitation. These findings indicate that AF1q up-regulation of BAD is through its effect on NF-kappaB and this may hint of its oncogenic mechanism in cancer.</abstract><cop>Greece</cop><pmid>20596645</pmid><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1791-2431 |
| ispartof | Oncology reports, 2010-08, Vol.24 (2), p.547-554 |
| issn | 1791-2431 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733628877 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Apoptosis - drug effects Apoptosis - genetics Apoptosis - radiation effects Base Sequence bcl-Associated Death Protein - genetics bcl-Associated Death Protein - metabolism Blood Proteins - antagonists & inhibitors Blood Proteins - genetics Blood Proteins - physiology Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Cell Line, Tumor Gamma Rays Gene Expression Regulation, Neoplastic - drug effects Gene Expression Regulation, Neoplastic - radiation effects Humans Molecular Sequence Data Neoplasm Proteins - antagonists & inhibitors Neoplasm Proteins - genetics Neoplasm Proteins - physiology NF-kappa B - antagonists & inhibitors NF-kappa B - genetics NF-kappa B - metabolism NF-kappa B - physiology Promoter Regions, Genetic Protein Binding Proto-Oncogene Proteins RNA, Small Interfering - pharmacology Transcription, Genetic - drug effects Transcription, Genetic - genetics Up-Regulation - drug effects Up-Regulation - genetics |
| title | AF1q enhancement of gamma irradiation-induced apoptosis by up-regulation of BAD expression via NF-kappaB in human squamous carcinoma A431 cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T11%3A09%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=AF1q%20enhancement%20of%20gamma%20irradiation-induced%20apoptosis%20by%20up-regulation%20of%20BAD%20expression%20via%20NF-kappaB%20in%20human%20squamous%20carcinoma%20A431%20cells&rft.jtitle=Oncology%20reports&rft.au=Co,%20Ngai%20Na&rft.date=2010-08&rft.volume=24&rft.issue=2&rft.spage=547&rft.epage=554&rft.pages=547-554&rft.eissn=1791-2431&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E733628877%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733628877&rft_id=info:pmid/20596645&rfr_iscdi=true |