Purification and Characterization of Enterocin LR/6, a Bacteriocin from Enterococcus faecium LR/6
Enterocin LR/6, a bacteriocin obtained from the culture filtrate of Enterococcus faecium strain LR/6, has been purified to homogeneity using ammonium sulfate precipitation, cation-exchange chromatography, gel-filtration, and checked on reverse-phase high-performance liquid chromatography. It is acti...
Gespeichert in:
| Veröffentlicht in: | Applied biochemistry and biotechnology 2010, Vol.160 (1), p.40-49 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 49 |
|---|---|
| container_issue | 1 |
| container_start_page | 40 |
| container_title | Applied biochemistry and biotechnology |
| container_volume | 160 |
| creator | Kumar, Manoj Tiwari, Santosh Kumar Srivastava, Sheela |
| description | Enterocin LR/6, a bacteriocin obtained from the culture filtrate of Enterococcus faecium strain LR/6, has been purified to homogeneity using ammonium sulfate precipitation, cation-exchange chromatography, gel-filtration, and checked on reverse-phase high-performance liquid chromatography. It is active at high temperatures (boiling as well as autoclaving) and over a wide range of pH (2.0-8.0). Also, it is sensitive to a number of proteolytic enzymes but is stable in the presence of surfactants and organic solvents. The protein could be stored at least up to 1 year at low temperatures (4 °C and −20 °C) without any loss of activity. The N-terminal sequence of enterocin LR/6 showed no homology with known enterocins or other bacteriocins present in the database, suggesting it to be a novel enterocin. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed its mass to be ∼6.1 kDa. It showed a bactericidal mode of action against indicator strain, Micrococcus luteus. |
| doi_str_mv | 10.1007/s12010-009-8586-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733624361</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>21262476</sourcerecordid><originalsourceid>FETCH-LOGICAL-c455t-2849e92def10c3dd2fc0364825effe7e482199311be11b06ca1ccc6b4429cef33</originalsourceid><addsrcrecordid>eNp90cFu1DAQBmALgeiy8ABcIEICLoTOjGM7PsKqtJVWAgE9W17HLqk2cbE3B_bpcZulIA49WLEn30wc_Yw9R3iPAOo4IwFCDaDrVrSy3j9gCxRC10AaH7IFkOI1UauP2JOcrwCQWqEesyPUpFTT8gWzX6bUh97ZXR_Hyo5dtfphk3U7n_r9XIyhOhnLObp-rNZfj-W7ylYfZ3JbCykOf0h0bspVsN7103Crn7JHwW6zf3Z4LtnFp5Pvq7N6_fn0fPVhXbtGiF1NbaO9ps4HBMe7joIDLpuWhA_BK192qDVH3PiyQDqLzjm5aRrSzgfOl-ztPPc6xZ-Tzzsz9Nn57daOPk7ZKM4lNVxikW_ulYRUpJIFvvoPXsUpjeUvDGqF5UJCF4QzcinmnHww16kfbPplEMxNTGaOyZSYzE1MZl96XhwGT5vBd387DrkU8PoAbHZ2G5IdXZ_vHJEEIQpcMppdLq_GS5_-ueE9X385NwUbjb1MZfDFt4I4oCIEIv4bgluyfA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>197121959</pqid></control><display><type>article</type><title>Purification and Characterization of Enterocin LR/6, a Bacteriocin from Enterococcus faecium LR/6</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Kumar, Manoj ; Tiwari, Santosh Kumar ; Srivastava, Sheela</creator><creatorcontrib>Kumar, Manoj ; Tiwari, Santosh Kumar ; Srivastava, Sheela</creatorcontrib><description>Enterocin LR/6, a bacteriocin obtained from the culture filtrate of Enterococcus faecium strain LR/6, has been purified to homogeneity using ammonium sulfate precipitation, cation-exchange chromatography, gel-filtration, and checked on reverse-phase high-performance liquid chromatography. It is active at high temperatures (boiling as well as autoclaving) and over a wide range of pH (2.0-8.0). Also, it is sensitive to a number of proteolytic enzymes but is stable in the presence of surfactants and organic solvents. The protein could be stored at least up to 1 year at low temperatures (4 °C and −20 °C) without any loss of activity. The N-terminal sequence of enterocin LR/6 showed no homology with known enterocins or other bacteriocins present in the database, suggesting it to be a novel enterocin. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed its mass to be ∼6.1 kDa. It showed a bactericidal mode of action against indicator strain, Micrococcus luteus.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-009-8586-z</identifier><identifier>PMID: 19277483</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>New York: New York : Humana Press Inc</publisher><subject>Amino Acid Sequence ; Ammonium ; ammonium sulfate ; Anti-Infective Agents - chemistry ; Anti-Infective Agents - isolation & purification ; Anti-Infective Agents - metabolism ; Anti-Infective Agents - pharmacology ; antibacterial properties ; Antibiotics ; Bacteria ; Bacteriocins - chemistry ; Bacteriocins - isolation & purification ; Bacteriocins - metabolism ; Bacteriocins - pharmacology ; Biochemistry ; Biological and medical sciences ; Biotechnology ; Cell Survival - drug effects ; chemical precipitation ; Chemistry ; Chemistry and Materials Science ; enterocins ; Enterococcus faecium ; Enterococcus faecium - chemistry ; Enzymes ; Filtrate ; Fundamental and applied biological sciences. Psychology ; high performance liquid chromatography ; High temperature ; Hot Temperature ; Hydrogen-Ion Concentration ; Hydrolysis ; Ionization ; Kinetics ; Liquid chromatography ; Low temperature ; Mass spectrometry ; Micrococcus luteus ; Micrococcus luteus - cytology ; Micrococcus luteus - drug effects ; Mode of action ; Molecular Weight ; Organic Chemicals - pharmacology ; Organic solvents ; Peptide Hydrolases - metabolism ; Protein Stability - drug effects ; Proteins ; purification ; Solvents - pharmacology ; storage temperature ; Sulfates ; Surface-Active Agents - pharmacology</subject><ispartof>Applied biochemistry and biotechnology, 2010, Vol.160 (1), p.40-49</ispartof><rights>Humana Press 2009</rights><rights>2015 INIST-CNRS</rights><rights>Springer Science+Business Media, LLC 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-2849e92def10c3dd2fc0364825effe7e482199311be11b06ca1ccc6b4429cef33</citedby><cites>FETCH-LOGICAL-c455t-2849e92def10c3dd2fc0364825effe7e482199311be11b06ca1ccc6b4429cef33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-009-8586-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-009-8586-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,4009,27902,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22605548$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19277483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumar, Manoj</creatorcontrib><creatorcontrib>Tiwari, Santosh Kumar</creatorcontrib><creatorcontrib>Srivastava, Sheela</creatorcontrib><title>Purification and Characterization of Enterocin LR/6, a Bacteriocin from Enterococcus faecium LR/6</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Enterocin LR/6, a bacteriocin obtained from the culture filtrate of Enterococcus faecium strain LR/6, has been purified to homogeneity using ammonium sulfate precipitation, cation-exchange chromatography, gel-filtration, and checked on reverse-phase high-performance liquid chromatography. It is active at high temperatures (boiling as well as autoclaving) and over a wide range of pH (2.0-8.0). Also, it is sensitive to a number of proteolytic enzymes but is stable in the presence of surfactants and organic solvents. The protein could be stored at least up to 1 year at low temperatures (4 °C and −20 °C) without any loss of activity. The N-terminal sequence of enterocin LR/6 showed no homology with known enterocins or other bacteriocins present in the database, suggesting it to be a novel enterocin. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed its mass to be ∼6.1 kDa. It showed a bactericidal mode of action against indicator strain, Micrococcus luteus.</description><subject>Amino Acid Sequence</subject><subject>Ammonium</subject><subject>ammonium sulfate</subject><subject>Anti-Infective Agents - chemistry</subject><subject>Anti-Infective Agents - isolation & purification</subject><subject>Anti-Infective Agents - metabolism</subject><subject>Anti-Infective Agents - pharmacology</subject><subject>antibacterial properties</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacteriocins - chemistry</subject><subject>Bacteriocins - isolation & purification</subject><subject>Bacteriocins - metabolism</subject><subject>Bacteriocins - pharmacology</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Survival - drug effects</subject><subject>chemical precipitation</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>enterocins</subject><subject>Enterococcus faecium</subject><subject>Enterococcus faecium - chemistry</subject><subject>Enzymes</subject><subject>Filtrate</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>high performance liquid chromatography</subject><subject>High temperature</subject><subject>Hot Temperature</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolysis</subject><subject>Ionization</subject><subject>Kinetics</subject><subject>Liquid chromatography</subject><subject>Low temperature</subject><subject>Mass spectrometry</subject><subject>Micrococcus luteus</subject><subject>Micrococcus luteus - cytology</subject><subject>Micrococcus luteus - drug effects</subject><subject>Mode of action</subject><subject>Molecular Weight</subject><subject>Organic Chemicals - pharmacology</subject><subject>Organic solvents</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Protein Stability - drug effects</subject><subject>Proteins</subject><subject>purification</subject><subject>Solvents - pharmacology</subject><subject>storage temperature</subject><subject>Sulfates</subject><subject>Surface-Active Agents - pharmacology</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90cFu1DAQBmALgeiy8ABcIEICLoTOjGM7PsKqtJVWAgE9W17HLqk2cbE3B_bpcZulIA49WLEn30wc_Yw9R3iPAOo4IwFCDaDrVrSy3j9gCxRC10AaH7IFkOI1UauP2JOcrwCQWqEesyPUpFTT8gWzX6bUh97ZXR_Hyo5dtfphk3U7n_r9XIyhOhnLObp-rNZfj-W7ylYfZ3JbCykOf0h0bspVsN7103Crn7JHwW6zf3Z4LtnFp5Pvq7N6_fn0fPVhXbtGiF1NbaO9ps4HBMe7joIDLpuWhA_BK192qDVH3PiyQDqLzjm5aRrSzgfOl-ztPPc6xZ-Tzzsz9Nn57daOPk7ZKM4lNVxikW_ulYRUpJIFvvoPXsUpjeUvDGqF5UJCF4QzcinmnHww16kfbPplEMxNTGaOyZSYzE1MZl96XhwGT5vBd387DrkU8PoAbHZ2G5IdXZ_vHJEEIQpcMppdLq_GS5_-ueE9X385NwUbjb1MZfDFt4I4oCIEIv4bgluyfA</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Kumar, Manoj</creator><creator>Tiwari, Santosh Kumar</creator><creator>Srivastava, Sheela</creator><general>New York : Humana Press Inc</general><general>Humana Press Inc</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7QL</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>2010</creationdate><title>Purification and Characterization of Enterocin LR/6, a Bacteriocin from Enterococcus faecium LR/6</title><author>Kumar, Manoj ; Tiwari, Santosh Kumar ; Srivastava, Sheela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-2849e92def10c3dd2fc0364825effe7e482199311be11b06ca1ccc6b4429cef33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino Acid Sequence</topic><topic>Ammonium</topic><topic>ammonium sulfate</topic><topic>Anti-Infective Agents - chemistry</topic><topic>Anti-Infective Agents - isolation & purification</topic><topic>Anti-Infective Agents - metabolism</topic><topic>Anti-Infective Agents - pharmacology</topic><topic>antibacterial properties</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacteriocins - chemistry</topic><topic>Bacteriocins - isolation & purification</topic><topic>Bacteriocins - metabolism</topic><topic>Bacteriocins - pharmacology</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Survival - drug effects</topic><topic>chemical precipitation</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>enterocins</topic><topic>Enterococcus faecium</topic><topic>Enterococcus faecium - chemistry</topic><topic>Enzymes</topic><topic>Filtrate</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>high performance liquid chromatography</topic><topic>High temperature</topic><topic>Hot Temperature</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydrolysis</topic><topic>Ionization</topic><topic>Kinetics</topic><topic>Liquid chromatography</topic><topic>Low temperature</topic><topic>Mass spectrometry</topic><topic>Micrococcus luteus</topic><topic>Micrococcus luteus - cytology</topic><topic>Micrococcus luteus - drug effects</topic><topic>Mode of action</topic><topic>Molecular Weight</topic><topic>Organic Chemicals - pharmacology</topic><topic>Organic solvents</topic><topic>Peptide Hydrolases - metabolism</topic><topic>Protein Stability - drug effects</topic><topic>Proteins</topic><topic>purification</topic><topic>Solvents - pharmacology</topic><topic>storage temperature</topic><topic>Sulfates</topic><topic>Surface-Active Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, Manoj</creatorcontrib><creatorcontrib>Tiwari, Santosh Kumar</creatorcontrib><creatorcontrib>Srivastava, Sheela</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, Manoj</au><au>Tiwari, Santosh Kumar</au><au>Srivastava, Sheela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and Characterization of Enterocin LR/6, a Bacteriocin from Enterococcus faecium LR/6</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2010</date><risdate>2010</risdate><volume>160</volume><issue>1</issue><spage>40</spage><epage>49</epage><pages>40-49</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><coden>ABIBDL</coden><abstract>Enterocin LR/6, a bacteriocin obtained from the culture filtrate of Enterococcus faecium strain LR/6, has been purified to homogeneity using ammonium sulfate precipitation, cation-exchange chromatography, gel-filtration, and checked on reverse-phase high-performance liquid chromatography. It is active at high temperatures (boiling as well as autoclaving) and over a wide range of pH (2.0-8.0). Also, it is sensitive to a number of proteolytic enzymes but is stable in the presence of surfactants and organic solvents. The protein could be stored at least up to 1 year at low temperatures (4 °C and −20 °C) without any loss of activity. The N-terminal sequence of enterocin LR/6 showed no homology with known enterocins or other bacteriocins present in the database, suggesting it to be a novel enterocin. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed its mass to be ∼6.1 kDa. It showed a bactericidal mode of action against indicator strain, Micrococcus luteus.</abstract><cop>New York</cop><pub>New York : Humana Press Inc</pub><pmid>19277483</pmid><doi>10.1007/s12010-009-8586-z</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0273-2289 |
| ispartof | Applied biochemistry and biotechnology, 2010, Vol.160 (1), p.40-49 |
| issn | 0273-2289 1559-0291 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733624361 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Amino Acid Sequence Ammonium ammonium sulfate Anti-Infective Agents - chemistry Anti-Infective Agents - isolation & purification Anti-Infective Agents - metabolism Anti-Infective Agents - pharmacology antibacterial properties Antibiotics Bacteria Bacteriocins - chemistry Bacteriocins - isolation & purification Bacteriocins - metabolism Bacteriocins - pharmacology Biochemistry Biological and medical sciences Biotechnology Cell Survival - drug effects chemical precipitation Chemistry Chemistry and Materials Science enterocins Enterococcus faecium Enterococcus faecium - chemistry Enzymes Filtrate Fundamental and applied biological sciences. Psychology high performance liquid chromatography High temperature Hot Temperature Hydrogen-Ion Concentration Hydrolysis Ionization Kinetics Liquid chromatography Low temperature Mass spectrometry Micrococcus luteus Micrococcus luteus - cytology Micrococcus luteus - drug effects Mode of action Molecular Weight Organic Chemicals - pharmacology Organic solvents Peptide Hydrolases - metabolism Protein Stability - drug effects Proteins purification Solvents - pharmacology storage temperature Sulfates Surface-Active Agents - pharmacology |
| title | Purification and Characterization of Enterocin LR/6, a Bacteriocin from Enterococcus faecium LR/6 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T22%3A27%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Purification%20and%20Characterization%20of%20Enterocin%20LR/6,%20a%20Bacteriocin%20from%20Enterococcus%20faecium%20LR/6&rft.jtitle=Applied%20biochemistry%20and%20biotechnology&rft.au=Kumar,%20Manoj&rft.date=2010&rft.volume=160&rft.issue=1&rft.spage=40&rft.epage=49&rft.pages=40-49&rft.issn=0273-2289&rft.eissn=1559-0291&rft.coden=ABIBDL&rft_id=info:doi/10.1007/s12010-009-8586-z&rft_dat=%3Cproquest_cross%3E21262476%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=197121959&rft_id=info:pmid/19277483&rfr_iscdi=true |