Fluorescence aptasensor based on competitive-binding for human neutrophil elastase detection

To our knowledge, we report the first fluorescence aptasensor for detecting human neutrophil elastase (HNE) in homogeneous solution. The biosensor contains a short DNA scrambled sequence strand (SS) complementary to part of the aptamer sequence or the loop of molecular beacon (MB). The aptamer-HNE r...

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Veröffentlicht in:	Talanta (Oxford) 2010, Vol.80 (3), p.1264-1268
Hauptverfasser:	He, Jing-Lin, Wu, Zai-Sheng, Zhang, Song-Bai, Shen, Guo-Li, Yu, Ru-Qin
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Aptamers, Nucleotide - genetics Aptamers, Nucleotide - metabolism Aptasensor Base Sequence Binding Sites Binding, Competitive Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Chemistry Competitive-binding reaction DNA - chemistry DNA - genetics DNA - metabolism Exact sciences and technology Fluorescein - metabolism Fluorescence Fundamental and applied biological sciences. Psychology General, instrumentation Human neutrophil elastase Humans Leukocyte Elastase - analysis Leukocyte Elastase - metabolism Methods. Procedures. Technologies Molecular beacon Solutions Spectrometric and optical methods Spectrometry, Fluorescence Various methods and equipments
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