Antioxidant Enzyme Expression, Lipid Peroxidation, and Protein Oxidation in Human Myometrium With Parturition
Oxygen levels fluctuate considerably during human labor leading to hypoxia and reoxygenation of the uteroplacental unit and in some cases may compromise the progression of labor. Our aim was to assess the possible contribution of oxidative stress to the onset of labor. Thiobarbituric acid was used a...
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| Veröffentlicht in: | Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2010-01, Vol.17 (1), p.78-84 |
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| creator | Khan, Raheela N. Matharoo-Ball, B. Shaw, R.W. |
| description | Oxygen levels fluctuate considerably during human labor leading to hypoxia and reoxygenation of the uteroplacental unit and in some cases may compromise the progression of labor. Our aim was to assess the possible contribution of oxidative stress to the onset of labor. Thiobarbituric acid was used as a marker of lipid peroxidation along with Western blotting using anti-dinitrophenylhydrazine (DNPH) to assess protein carbonylation in myometrial samples obtained before and after the onset of term and preterm labor. Levels of key antioxidative enzymes were also compared. Higher levels of lipid peroxidation were observed in myometrial samples obtained during term or preterm labor. Reduced levels of glutathione peroxidase (GSHPx) were also encountered in these 2 groups. Conversely, protein carbonyl content was higher in laboring term and preterm myometrial samples. Levels of catalase (CAT) and superoxide dismutase (SOD) were unaltered across all 4 groups. Lipids in the laboring myometrium are susceptible to oxidative injury possibly due to diminished protection as a result of lower GSHPx activity. The reason for enhanced protein carbonylation suggests differential mechanisms governing protein turnover in the pregnant compared with the parturient uterus. Localized, oxidant damage of human myometrium may be a causal factor in difficult deliveries. |
| doi_str_mv | 10.1177/1933719109348027 |
| format | Article |
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Our aim was to assess the possible contribution of oxidative stress to the onset of labor. Thiobarbituric acid was used as a marker of lipid peroxidation along with Western blotting using anti-dinitrophenylhydrazine (DNPH) to assess protein carbonylation in myometrial samples obtained before and after the onset of term and preterm labor. Levels of key antioxidative enzymes were also compared. Higher levels of lipid peroxidation were observed in myometrial samples obtained during term or preterm labor. Reduced levels of glutathione peroxidase (GSHPx) were also encountered in these 2 groups. Conversely, protein carbonyl content was higher in laboring term and preterm myometrial samples. Levels of catalase (CAT) and superoxide dismutase (SOD) were unaltered across all 4 groups. Lipids in the laboring myometrium are susceptible to oxidative injury possibly due to diminished protection as a result of lower GSHPx activity. The reason for enhanced protein carbonylation suggests differential mechanisms governing protein turnover in the pregnant compared with the parturient uterus. Localized, oxidant damage of human myometrium may be a causal factor in difficult deliveries.</description><identifier>ISSN: 1933-7191</identifier><identifier>EISSN: 1933-7205</identifier><identifier>DOI: 10.1177/1933719109348027</identifier><identifier>PMID: 19801538</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Analysis of Variance ; Biological and medical sciences ; Blotting, Western ; Catalase - metabolism ; Delivery. Postpartum. Lactation ; Diseases of mother, fetus and pregnancy ; Embryology ; Female ; Glutathione Peroxidase - metabolism ; Gynecology. Andrology. Obstetrics ; Humans ; Hydrazines - metabolism ; Labor, Obstetric - metabolism ; Lipid Peroxidation - physiology ; Medical sciences ; Medicine & Public Health ; Myometrium - metabolism ; Obstetric Labor, Premature - metabolism ; Obstetrics/Perinatology/Midwifery ; Original Article ; Oxidative Stress - physiology ; Parturition - metabolism ; Pregnancy ; Pregnancy. Fetus. 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Sci</addtitle><addtitle>Reprod Sci</addtitle><description>Oxygen levels fluctuate considerably during human labor leading to hypoxia and reoxygenation of the uteroplacental unit and in some cases may compromise the progression of labor. Our aim was to assess the possible contribution of oxidative stress to the onset of labor. Thiobarbituric acid was used as a marker of lipid peroxidation along with Western blotting using anti-dinitrophenylhydrazine (DNPH) to assess protein carbonylation in myometrial samples obtained before and after the onset of term and preterm labor. Levels of key antioxidative enzymes were also compared. Higher levels of lipid peroxidation were observed in myometrial samples obtained during term or preterm labor. Reduced levels of glutathione peroxidase (GSHPx) were also encountered in these 2 groups. Conversely, protein carbonyl content was higher in laboring term and preterm myometrial samples. Levels of catalase (CAT) and superoxide dismutase (SOD) were unaltered across all 4 groups. Lipids in the laboring myometrium are susceptible to oxidative injury possibly due to diminished protection as a result of lower GSHPx activity. The reason for enhanced protein carbonylation suggests differential mechanisms governing protein turnover in the pregnant compared with the parturient uterus. Localized, oxidant damage of human myometrium may be a causal factor in difficult deliveries.</description><subject>Analysis of Variance</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Catalase - metabolism</subject><subject>Delivery. Postpartum. Lactation</subject><subject>Diseases of mother, fetus and pregnancy</subject><subject>Embryology</subject><subject>Female</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Hydrazines - metabolism</subject><subject>Labor, Obstetric - metabolism</subject><subject>Lipid Peroxidation - physiology</subject><subject>Medical sciences</subject><subject>Medicine & Public Health</subject><subject>Myometrium - metabolism</subject><subject>Obstetric Labor, Premature - metabolism</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Original Article</subject><subject>Oxidative Stress - physiology</subject><subject>Parturition - metabolism</subject><subject>Pregnancy</subject><subject>Pregnancy. Fetus. 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Postpartum. Lactation</topic><topic>Diseases of mother, fetus and pregnancy</topic><topic>Embryology</topic><topic>Female</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Hydrazines - metabolism</topic><topic>Labor, Obstetric - metabolism</topic><topic>Lipid Peroxidation - physiology</topic><topic>Medical sciences</topic><topic>Medicine & Public Health</topic><topic>Myometrium - metabolism</topic><topic>Obstetric Labor, Premature - metabolism</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Original Article</topic><topic>Oxidative Stress - physiology</topic><topic>Parturition - metabolism</topic><topic>Pregnancy</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>Protein Carbonylation - physiology</topic><topic>Reproductive Medicine</topic><topic>Superoxide Dismutase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khan, Raheela N.</creatorcontrib><creatorcontrib>Matharoo-Ball, B.</creatorcontrib><creatorcontrib>Shaw, R.W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Reproductive sciences (Thousand Oaks, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khan, Raheela N.</au><au>Matharoo-Ball, B.</au><au>Shaw, R.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant Enzyme Expression, Lipid Peroxidation, and Protein Oxidation in Human Myometrium With Parturition</atitle><jtitle>Reproductive sciences (Thousand Oaks, Calif.)</jtitle><stitle>Reprod. 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Reduced levels of glutathione peroxidase (GSHPx) were also encountered in these 2 groups. Conversely, protein carbonyl content was higher in laboring term and preterm myometrial samples. Levels of catalase (CAT) and superoxide dismutase (SOD) were unaltered across all 4 groups. Lipids in the laboring myometrium are susceptible to oxidative injury possibly due to diminished protection as a result of lower GSHPx activity. The reason for enhanced protein carbonylation suggests differential mechanisms governing protein turnover in the pregnant compared with the parturient uterus. Localized, oxidant damage of human myometrium may be a causal factor in difficult deliveries.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>19801538</pmid><doi>10.1177/1933719109348027</doi><tpages>7</tpages></addata></record> |
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| subjects | Analysis of Variance Biological and medical sciences Blotting, Western Catalase - metabolism Delivery. Postpartum. Lactation Diseases of mother, fetus and pregnancy Embryology Female Glutathione Peroxidase - metabolism Gynecology. Andrology. Obstetrics Humans Hydrazines - metabolism Labor, Obstetric - metabolism Lipid Peroxidation - physiology Medical sciences Medicine & Public Health Myometrium - metabolism Obstetric Labor, Premature - metabolism Obstetrics/Perinatology/Midwifery Original Article Oxidative Stress - physiology Parturition - metabolism Pregnancy Pregnancy. Fetus. Placenta Protein Carbonylation - physiology Reproductive Medicine Superoxide Dismutase - metabolism |
| title | Antioxidant Enzyme Expression, Lipid Peroxidation, and Protein Oxidation in Human Myometrium With Parturition |
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