Long-Term Engraftment of Fresh Human Myeloma Cells in SCID Mice
Using highly purified myeloma cells from patient bone marrow, we established human-murine myeloma chimeras in severe combined immunodeficiency (SCID) mice and documented secretion of monoclonal human immunoglobulins (HuIgs) in the mice for up to 299 days. Monoclonality of circulating HuIgs was found...
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| Veröffentlicht in: | Blood 1992-12, Vol.80 (11), p.2843-2850 |
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| creator | Feo-Zuppardi, Francisco J. Taylor, Charles W. Iwato, Koji Lopez, Marialouisa H.A. Grogan, Thomas M. Odeleye, Abiodun Hersh, Evan M. Salmon, Sydney E. |
| description | Using highly purified myeloma cells from patient bone marrow, we established human-murine myeloma chimeras in severe combined immunodeficiency (SCID) mice and documented secretion of monoclonal human immunoglobulins (HuIgs) in the mice for up to 299 days. Monoclonality of circulating HuIgs was found only when highly purified myeloma cells were injected intraperitoneally. In contrast, injection of unfractionated myeloma marrow led to the development of polyclonal HuIgs in the SCID mice. The criteria for myeloma engraftment included prolonged presence of monoclonal HuIgs in the sera of SCID mice and/or detection of human myeloma cells in their tissues by immunohistochemical examination. Ninety-one percent (10/11) of the fresh purified myeloma specimens engrafted in the SCID mice. Fifty-five percent (6/11) of the patient samples resulted in human B-cell grafts, and 45% (5/11) were identifiable as human myeloma chimeras. Pathologic studies showed that most human plasmacytes were located in the peritoneal cavity but metastatic infiltrates were also found in other organs in 69% of the SCID-human myeloma chimeras. This chimeric model should provide a useful tool for characterization of growth modulation and microenvironmental interactions as well as a means of testing new therapeutic approaches to multiple myeloma. |
| doi_str_mv | 10.1182/blood.V80.11.2843.2843 |
| format | Article |
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Monoclonality of circulating HuIgs was found only when highly purified myeloma cells were injected intraperitoneally. In contrast, injection of unfractionated myeloma marrow led to the development of polyclonal HuIgs in the SCID mice. The criteria for myeloma engraftment included prolonged presence of monoclonal HuIgs in the sera of SCID mice and/or detection of human myeloma cells in their tissues by immunohistochemical examination. Ninety-one percent (10/11) of the fresh purified myeloma specimens engrafted in the SCID mice. Fifty-five percent (6/11) of the patient samples resulted in human B-cell grafts, and 45% (5/11) were identifiable as human myeloma chimeras. Pathologic studies showed that most human plasmacytes were located in the peritoneal cavity but metastatic infiltrates were also found in other organs in 69% of the SCID-human myeloma chimeras. This chimeric model should provide a useful tool for characterization of growth modulation and microenvironmental interactions as well as a means of testing new therapeutic approaches to multiple myeloma.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V80.11.2843.2843</identifier><identifier>PMID: 1450409</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Adult ; Aged ; Animal tumors. Experimental tumors ; Animals ; Biological and medical sciences ; Enzyme-Linked Immunosorbent Assay ; Experimental malignant blood diseases ; Female ; Humans ; Immunoenzyme Techniques ; Immunoglobulin G - analysis ; Immunoglobulin kappa-Chains - analysis ; Immunoglobulin Light Chains - analysis ; Male ; Medical sciences ; Mice ; Mice, SCID ; Middle Aged ; Multiple Myeloma - immunology ; Multiple Myeloma - pathology ; Neoplasm Staging ; Neoplasm Transplantation ; Transplantation, Heterologous ; Tumors</subject><ispartof>Blood, 1992-12, Vol.80 (11), p.2843-2850</ispartof><rights>1992 American Society of Hematology</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-8206f69ff28bd1e3b61e9962c70e8b860bcec68316271dcbd4ebca6a7a39fdfc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4507815$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1450409$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feo-Zuppardi, Francisco J.</creatorcontrib><creatorcontrib>Taylor, Charles W.</creatorcontrib><creatorcontrib>Iwato, Koji</creatorcontrib><creatorcontrib>Lopez, Marialouisa H.A.</creatorcontrib><creatorcontrib>Grogan, Thomas M.</creatorcontrib><creatorcontrib>Odeleye, Abiodun</creatorcontrib><creatorcontrib>Hersh, Evan M.</creatorcontrib><creatorcontrib>Salmon, Sydney E.</creatorcontrib><title>Long-Term Engraftment of Fresh Human Myeloma Cells in SCID Mice</title><title>Blood</title><addtitle>Blood</addtitle><description>Using highly purified myeloma cells from patient bone marrow, we established human-murine myeloma chimeras in severe combined immunodeficiency (SCID) mice and documented secretion of monoclonal human immunoglobulins (HuIgs) in the mice for up to 299 days. Monoclonality of circulating HuIgs was found only when highly purified myeloma cells were injected intraperitoneally. In contrast, injection of unfractionated myeloma marrow led to the development of polyclonal HuIgs in the SCID mice. The criteria for myeloma engraftment included prolonged presence of monoclonal HuIgs in the sera of SCID mice and/or detection of human myeloma cells in their tissues by immunohistochemical examination. Ninety-one percent (10/11) of the fresh purified myeloma specimens engrafted in the SCID mice. Fifty-five percent (6/11) of the patient samples resulted in human B-cell grafts, and 45% (5/11) were identifiable as human myeloma chimeras. Pathologic studies showed that most human plasmacytes were located in the peritoneal cavity but metastatic infiltrates were also found in other organs in 69% of the SCID-human myeloma chimeras. This chimeric model should provide a useful tool for characterization of growth modulation and microenvironmental interactions as well as a means of testing new therapeutic approaches to multiple myeloma.</description><subject>Adult</subject><subject>Aged</subject><subject>Animal tumors. Experimental tumors</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Experimental malignant blood diseases</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Immunoglobulin G - analysis</subject><subject>Immunoglobulin kappa-Chains - analysis</subject><subject>Immunoglobulin Light Chains - analysis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>Middle Aged</subject><subject>Multiple Myeloma - immunology</subject><subject>Multiple Myeloma - pathology</subject><subject>Neoplasm Staging</subject><subject>Neoplasm Transplantation</subject><subject>Transplantation, Heterologous</subject><subject>Tumors</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAQgIMouj5-gpKDeOuapG2anlTqrgorHnxcQ5pONNI2mrSC_97sAz16mWGYbx58CJ1QMqVUsPO6da6ZvohlOWUiS1dhC01ozkRCCCPbaEII4UlWFnQP7YfwTgjNUpbvol2a5SQj5QRdLFz_mjyB7_Csf_XKDB30A3YGzz2EN3w7dqrH99_Quk7hCto2YNvjx-ruGt9bDYdox6g2wNEmH6Dn-eypuk0WDzd31dUi0VlGhkQwwg0vjWGibiikNadQlpzpgoCoBSe1Bs1FSjkraKPrJoNaK64KlZamMTo9QGfrvR_efY4QBtnZoOM7qgc3BlmkKaelEBHka1B7F4IHIz-87ZT_lpTIpTm5MiejuVjKpbRViIPHmwtj3UHzN7ZWFfunm74KWrXGq17b8ItFqhA0j9jlGoNo48uCl0Fb6DU01oMeZOPsf5_8AD4HjC4</recordid><startdate>19921201</startdate><enddate>19921201</enddate><creator>Feo-Zuppardi, Francisco J.</creator><creator>Taylor, Charles W.</creator><creator>Iwato, Koji</creator><creator>Lopez, Marialouisa H.A.</creator><creator>Grogan, Thomas M.</creator><creator>Odeleye, Abiodun</creator><creator>Hersh, Evan M.</creator><creator>Salmon, Sydney E.</creator><general>Elsevier Inc</general><general>The Americain Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19921201</creationdate><title>Long-Term Engraftment of Fresh Human Myeloma Cells in SCID Mice</title><author>Feo-Zuppardi, Francisco J. ; Taylor, Charles W. ; Iwato, Koji ; Lopez, Marialouisa H.A. ; Grogan, Thomas M. ; Odeleye, Abiodun ; Hersh, Evan M. ; Salmon, Sydney E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-8206f69ff28bd1e3b61e9962c70e8b860bcec68316271dcbd4ebca6a7a39fdfc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Animal tumors. Experimental tumors</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Experimental malignant blood diseases</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Immunoglobulin G - analysis</topic><topic>Immunoglobulin kappa-Chains - analysis</topic><topic>Immunoglobulin Light Chains - analysis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, SCID</topic><topic>Middle Aged</topic><topic>Multiple Myeloma - immunology</topic><topic>Multiple Myeloma - pathology</topic><topic>Neoplasm Staging</topic><topic>Neoplasm Transplantation</topic><topic>Transplantation, Heterologous</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feo-Zuppardi, Francisco J.</creatorcontrib><creatorcontrib>Taylor, Charles W.</creatorcontrib><creatorcontrib>Iwato, Koji</creatorcontrib><creatorcontrib>Lopez, Marialouisa H.A.</creatorcontrib><creatorcontrib>Grogan, Thomas M.</creatorcontrib><creatorcontrib>Odeleye, Abiodun</creatorcontrib><creatorcontrib>Hersh, Evan M.</creatorcontrib><creatorcontrib>Salmon, Sydney E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feo-Zuppardi, Francisco J.</au><au>Taylor, Charles W.</au><au>Iwato, Koji</au><au>Lopez, Marialouisa H.A.</au><au>Grogan, Thomas M.</au><au>Odeleye, Abiodun</au><au>Hersh, Evan M.</au><au>Salmon, Sydney E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long-Term Engraftment of Fresh Human Myeloma Cells in SCID Mice</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1992-12-01</date><risdate>1992</risdate><volume>80</volume><issue>11</issue><spage>2843</spage><epage>2850</epage><pages>2843-2850</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>Using highly purified myeloma cells from patient bone marrow, we established human-murine myeloma chimeras in severe combined immunodeficiency (SCID) mice and documented secretion of monoclonal human immunoglobulins (HuIgs) in the mice for up to 299 days. Monoclonality of circulating HuIgs was found only when highly purified myeloma cells were injected intraperitoneally. In contrast, injection of unfractionated myeloma marrow led to the development of polyclonal HuIgs in the SCID mice. The criteria for myeloma engraftment included prolonged presence of monoclonal HuIgs in the sera of SCID mice and/or detection of human myeloma cells in their tissues by immunohistochemical examination. Ninety-one percent (10/11) of the fresh purified myeloma specimens engrafted in the SCID mice. Fifty-five percent (6/11) of the patient samples resulted in human B-cell grafts, and 45% (5/11) were identifiable as human myeloma chimeras. Pathologic studies showed that most human plasmacytes were located in the peritoneal cavity but metastatic infiltrates were also found in other organs in 69% of the SCID-human myeloma chimeras. 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| ispartof | Blood, 1992-12, Vol.80 (11), p.2843-2850 |
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| language | eng |
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| subjects | Adult Aged Animal tumors. Experimental tumors Animals Biological and medical sciences Enzyme-Linked Immunosorbent Assay Experimental malignant blood diseases Female Humans Immunoenzyme Techniques Immunoglobulin G - analysis Immunoglobulin kappa-Chains - analysis Immunoglobulin Light Chains - analysis Male Medical sciences Mice Mice, SCID Middle Aged Multiple Myeloma - immunology Multiple Myeloma - pathology Neoplasm Staging Neoplasm Transplantation Transplantation, Heterologous Tumors |
| title | Long-Term Engraftment of Fresh Human Myeloma Cells in SCID Mice |
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