Mathematical analysis of two-phase mass transfer in a batch reactor for the chemical transformation of a steroid

A reactor is described for the conversion of the slightly water‐soluble steroid testosterone (T) to 4‐androstene‐3, 17‐dione (4‐AD) by enzyme in the presence of excess cofactor. Since the enzyme is subject to substrate inhibition, reaction rates are strong functions of aqueous substrate concentratio...

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Veröffentlicht in:	Biotechnology and bioengineering 1987-09, Vol.30 (4), p.505-513
Hauptverfasser:	Pereira, Vincent, Tigli, Husevin, Gryte, Carl C.
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Sprache:	eng
Schlagworte:	4-androstene-3,17-dione bioreactors mass transfer mathematical models testosterone
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container_title	Biotechnology and bioengineering
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creator	Pereira, Vincent Tigli, Husevin Gryte, Carl C.
description	A reactor is described for the conversion of the slightly water‐soluble steroid testosterone (T) to 4‐androstene‐3, 17‐dione (4‐AD) by enzyme in the presence of excess cofactor. Since the enzyme is subject to substrate inhibition, reaction rates are strong functions of aqueous substrate concentration. High concentrations of the substrate, testosterone, per unit reactor volume are maintained within poly(dimethylsiloxane) beads that are suspended in the aqueous enzyme solution. Mass transfer (controlled by bead size, polymer to water volume ratio, enzyme loading) is used to control the degree and rate of conversion. The reactor dynamics are predicted over a wide range of reaction conditions. The product steroid is recovered in the polymeric beads from the enzyme solution.
doi_str_mv	10.1002/bit.260300407
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Bioeng</addtitle><date>1987-09</date><risdate>1987</risdate><volume>30</volume><issue>4</issue><spage>505</spage><epage>513</epage><pages>505-513</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><abstract>A reactor is described for the conversion of the slightly water‐soluble steroid testosterone (T) to 4‐androstene‐3, 17‐dione (4‐AD) by enzyme in the presence of excess cofactor. Since the enzyme is subject to substrate inhibition, reaction rates are strong functions of aqueous substrate concentration. High concentrations of the substrate, testosterone, per unit reactor volume are maintained within poly(dimethylsiloxane) beads that are suspended in the aqueous enzyme solution. Mass transfer (controlled by bead size, polymer to water volume ratio, enzyme loading) is used to control the degree and rate of conversion. The reactor dynamics are predicted over a wide range of reaction conditions. 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subjects	4-androstene-3,17-dione bioreactors mass transfer mathematical models testosterone
title	Mathematical analysis of two-phase mass transfer in a batch reactor for the chemical transformation of a steroid
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