Ionic binding of biologically active proteins on cross-linked acrylic macromolecular supports
The immobilization process of some biologically active proteins on acrylic ion exchange supports was studied. The cross‐linking and swelling coefficient, as well as the molecular weight of the enzyme, were proved to influence the immobilization process. The kinetic data indicate mainly a binding pro...
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| Veröffentlicht in: | Biotechnology and bioengineering 1987-10, Vol.30 (5), p.593-597 |
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| container_end_page | 597 |
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| container_title | Biotechnology and bioengineering |
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| creator | Maxim, Steliana Flondor, Aurelia Carpov, Adrian |
| description | The immobilization process of some biologically active proteins on acrylic ion exchange supports was studied. The cross‐linking and swelling coefficient, as well as the molecular weight of the enzyme, were proved to influence the immobilization process. The kinetic data indicate mainly a binding process controlled by diffusion. The uptake coefficient values of different proteins ranged between 35.0 and 98.0%. The laboratory experiments per formed with some of the obtained enzymic preparation certify the preservation of the catalytic activity following the immobilization process. |
| doi_str_mv | 10.1002/bit.260300502 |
| format | Article |
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The cross‐linking and swelling coefficient, as well as the molecular weight of the enzyme, were proved to influence the immobilization process. The kinetic data indicate mainly a binding process controlled by diffusion. The uptake coefficient values of different proteins ranged between 35.0 and 98.0%. The laboratory experiments per formed with some of the obtained enzymic preparation certify the preservation of the catalytic activity following the immobilization process.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.260300502</identifier><identifier>PMID: 18581445</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Biological and medical sciences ; Biotechnology ; Enzyme engineering ; Fundamental and applied biological sciences. 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Bioeng</addtitle><description>The immobilization process of some biologically active proteins on acrylic ion exchange supports was studied. The cross‐linking and swelling coefficient, as well as the molecular weight of the enzyme, were proved to influence the immobilization process. The kinetic data indicate mainly a binding process controlled by diffusion. The uptake coefficient values of different proteins ranged between 35.0 and 98.0%. The laboratory experiments per formed with some of the obtained enzymic preparation certify the preservation of the catalytic activity following the immobilization process.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Enzyme engineering</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immobilization of enzymes and other molecules</subject><subject>Immobilization techniques</subject><subject>Methods. Procedures. 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| ispartof | Biotechnology and bioengineering, 1987-10, Vol.30 (5), p.593-597 |
| issn | 0006-3592 1097-0290 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733619472 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences Biotechnology Enzyme engineering Fundamental and applied biological sciences. Psychology Immobilization of enzymes and other molecules Immobilization techniques Methods. Procedures. Technologies |
| title | Ionic binding of biologically active proteins on cross-linked acrylic macromolecular supports |
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