An enzyme electrode for on-line determination of ethanol and methanol
Since a stable alcohol oxidase with a high specific activity is not commercially available, we propose to produce and purify this enzyme from a strain of the yeast Hansenula polymorpha. This alcohol oxidase was immobilized into a gelatin matrix and its activity was estimated by a pO2 sensor. The enz...
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Veröffentlicht in: | Biotechnol. Bioeng.; (United States) 1987-12, Vol.30 (9), p.1001-1005 |
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creator | Belghith, H Romette, J.L Thomas, D |
description | Since a stable alcohol oxidase with a high specific activity is not commercially available, we propose to produce and purify this enzyme from a strain of the yeast Hansenula polymorpha. This alcohol oxidase was immobilized into a gelatin matrix and its activity was estimated by a pO2 sensor. The enzyme electrode obtained was then used in a continuous flow system to measure methanol or ethanol concentrations. The sample oxygen content dependence of the signal was minimized by the support properties. Measuring time for each sample were less than two minutes including response data treatment and rinsing step. The enzyme electrode response was set for ethanol from 0.5mM to 15mM and for methanol from 10mM to 300mM. On repeated use, the electrode signal for 10mM of ethanol was stable for at least 500 assays. Analysis have been performed in different beverages such as wine and beer, and the results compared to those obtained with classical methods of analysis. |
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This alcohol oxidase was immobilized into a gelatin matrix and its activity was estimated by a pO2 sensor. The enzyme electrode obtained was then used in a continuous flow system to measure methanol or ethanol concentrations. The sample oxygen content dependence of the signal was minimized by the support properties. Measuring time for each sample were less than two minutes including response data treatment and rinsing step. The enzyme electrode response was set for ethanol from 0.5mM to 15mM and for methanol from 10mM to 300mM. On repeated use, the electrode signal for 10mM of ethanol was stable for at least 500 assays. Analysis have been performed in different beverages such as wine and beer, and the results compared to those obtained with classical methods of analysis.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.260300902</identifier><identifier>PMID: 18581543</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>09 BIOMASS FUELS ; 090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989) ; ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; alcohol oxidase ; ALCOHOLES ; ALCOHOLS ; ALCOOL ; Biological and medical sciences ; Biotechnology ; ELECTRODES ; ENZYME ACTIVITY ; ENZYMES ; ENZYMIC ACTIVITY ; ETHANOL ; FRACTIONATION ; Fundamental and applied biological sciences. Psychology ; FUNGI ; HANSENULA ; Hansenula polymorpha ; HYDROXY COMPOUNDS ; IMMOBILIZED ENZYMES ; Industrial applications and implications. Economical aspects ; METHANOL ; MICROORGANISMS ; ORGANIC COMPOUNDS ; OXIDOREDUCTASES ; PLANTS ; PRODUCCION ; PRODUCTION ; SEPARATION PROCESSES ; YEASTS ; YIELDS</subject><ispartof>Biotechnol. Bioeng.; (United States), 1987-12, Vol.30 (9), p.1001-1005</ispartof><rights>Copyright © 1987 John Wiley & Sons, Inc.</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4652-6bc7437a1e4506e679a6bbc4d068dbab6b4771e058cbb82dbf5bc3111c7b1a973</citedby><cites>FETCH-LOGICAL-c4652-6bc7437a1e4506e679a6bbc4d068dbab6b4771e058cbb82dbf5bc3111c7b1a973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.260300902$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.260300902$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,885,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7539576$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18581543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/7015389$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Belghith, H</creatorcontrib><creatorcontrib>Romette, J.L</creatorcontrib><creatorcontrib>Thomas, D</creatorcontrib><creatorcontrib>Universite de Technologie de Compiegne (France)</creatorcontrib><title>An enzyme electrode for on-line determination of ethanol and methanol</title><title>Biotechnol. Bioeng.; (United States)</title><addtitle>Biotechnol. Bioeng</addtitle><description>Since a stable alcohol oxidase with a high specific activity is not commercially available, we propose to produce and purify this enzyme from a strain of the yeast Hansenula polymorpha. This alcohol oxidase was immobilized into a gelatin matrix and its activity was estimated by a pO2 sensor. The enzyme electrode obtained was then used in a continuous flow system to measure methanol or ethanol concentrations. The sample oxygen content dependence of the signal was minimized by the support properties. Measuring time for each sample were less than two minutes including response data treatment and rinsing step. The enzyme electrode response was set for ethanol from 0.5mM to 15mM and for methanol from 10mM to 300mM. On repeated use, the electrode signal for 10mM of ethanol was stable for at least 500 assays. Analysis have been performed in different beverages such as wine and beer, and the results compared to those obtained with classical methods of analysis.</description><subject>09 BIOMASS FUELS</subject><subject>090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)</subject><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>alcohol oxidase</subject><subject>ALCOHOLES</subject><subject>ALCOHOLS</subject><subject>ALCOOL</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>ELECTRODES</subject><subject>ENZYME ACTIVITY</subject><subject>ENZYMES</subject><subject>ENZYMIC ACTIVITY</subject><subject>ETHANOL</subject><subject>FRACTIONATION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>FUNGI</subject><subject>HANSENULA</subject><subject>Hansenula polymorpha</subject><subject>HYDROXY COMPOUNDS</subject><subject>IMMOBILIZED ENZYMES</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>METHANOL</subject><subject>MICROORGANISMS</subject><subject>ORGANIC COMPOUNDS</subject><subject>OXIDOREDUCTASES</subject><subject>PLANTS</subject><subject>PRODUCCION</subject><subject>PRODUCTION</subject><subject>SEPARATION PROCESSES</subject><subject>YEASTS</subject><subject>YIELDS</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNp90U1v1DAQBuAIgehSOHJDKEKonFLGcfx1LFW7VCpwoBXcLNuZUENit3ZWsPx6jDZaOPVkjfR4_MpvVT0ncEwA2rfWz8ctBwqgoH1QrQgo0UCr4GG1AgDeUKbag-pJzt_LKCTnj6sDIpkkrKOr6uwk1Bh-byescUQ3p9hjPcRUx9CMPmDd44xp8sHMPoY6DjXONybEsTahr6dleFo9GsyY8dlyHlbX52dXp--by0_ri9OTy8Z1nLUNt050VBiCHQOOXCjDrXVdD1z21lhuOyEIApPOWtn2dmDWUUKIE5YYJehh9Wq3N-bZ6-z8jO7GxRBKci2AMCpVQW926DbFuw3mWU8-OxxHEzBushaUcqI6gCKP7pWkUyCA0wKbHXQp5pxw0LfJTyZtNQH9twZdatD7Gop_uSze2An7f3r59wJeL8BkZ8YhmeB83jvBqGKCFyZ27KcfcXv_o_rdxdX_CZbEPs_4a3_TpB-aCyqY_vJxrTtJ1px_YPpr8S92fjBRm2-phLn-LGVLJBAp6R_-CLXq</recordid><startdate>19871220</startdate><enddate>19871220</enddate><creator>Belghith, H</creator><creator>Romette, J.L</creator><creator>Thomas, D</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19871220</creationdate><title>An enzyme electrode for on-line determination of ethanol and methanol</title><author>Belghith, H ; Romette, J.L ; Thomas, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4652-6bc7437a1e4506e679a6bbc4d068dbab6b4771e058cbb82dbf5bc3111c7b1a973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>09 BIOMASS FUELS</topic><topic>090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)</topic><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>alcohol oxidase</topic><topic>ALCOHOLES</topic><topic>ALCOHOLS</topic><topic>ALCOOL</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>ELECTRODES</topic><topic>ENZYME ACTIVITY</topic><topic>ENZYMES</topic><topic>ENZYMIC ACTIVITY</topic><topic>ETHANOL</topic><topic>FRACTIONATION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>FUNGI</topic><topic>HANSENULA</topic><topic>Hansenula polymorpha</topic><topic>HYDROXY COMPOUNDS</topic><topic>IMMOBILIZED ENZYMES</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>METHANOL</topic><topic>MICROORGANISMS</topic><topic>ORGANIC COMPOUNDS</topic><topic>OXIDOREDUCTASES</topic><topic>PLANTS</topic><topic>PRODUCCION</topic><topic>PRODUCTION</topic><topic>SEPARATION PROCESSES</topic><topic>YEASTS</topic><topic>YIELDS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Belghith, H</creatorcontrib><creatorcontrib>Romette, J.L</creatorcontrib><creatorcontrib>Thomas, D</creatorcontrib><creatorcontrib>Universite de Technologie de Compiegne (France)</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biotechnol. Bioeng.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Belghith, H</au><au>Romette, J.L</au><au>Thomas, D</au><aucorp>Universite de Technologie de Compiegne (France)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An enzyme electrode for on-line determination of ethanol and methanol</atitle><jtitle>Biotechnol. Bioeng.; (United States)</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>1987-12-20</date><risdate>1987</risdate><volume>30</volume><issue>9</issue><spage>1001</spage><epage>1005</epage><pages>1001-1005</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>Since a stable alcohol oxidase with a high specific activity is not commercially available, we propose to produce and purify this enzyme from a strain of the yeast Hansenula polymorpha. This alcohol oxidase was immobilized into a gelatin matrix and its activity was estimated by a pO2 sensor. The enzyme electrode obtained was then used in a continuous flow system to measure methanol or ethanol concentrations. The sample oxygen content dependence of the signal was minimized by the support properties. Measuring time for each sample were less than two minutes including response data treatment and rinsing step. The enzyme electrode response was set for ethanol from 0.5mM to 15mM and for methanol from 10mM to 300mM. On repeated use, the electrode signal for 10mM of ethanol was stable for at least 500 assays. Analysis have been performed in different beverages such as wine and beer, and the results compared to those obtained with classical methods of analysis.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18581543</pmid><doi>10.1002/bit.260300902</doi><tpages>5</tpages></addata></record> |
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subjects | 09 BIOMASS FUELS 090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989) ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE alcohol oxidase ALCOHOLES ALCOHOLS ALCOOL Biological and medical sciences Biotechnology ELECTRODES ENZYME ACTIVITY ENZYMES ENZYMIC ACTIVITY ETHANOL FRACTIONATION Fundamental and applied biological sciences. Psychology FUNGI HANSENULA Hansenula polymorpha HYDROXY COMPOUNDS IMMOBILIZED ENZYMES Industrial applications and implications. Economical aspects METHANOL MICROORGANISMS ORGANIC COMPOUNDS OXIDOREDUCTASES PLANTS PRODUCCION PRODUCTION SEPARATION PROCESSES YEASTS YIELDS |
title | An enzyme electrode for on-line determination of ethanol and methanol |
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