An enzyme electrode for on-line determination of ethanol and methanol

Since a stable alcohol oxidase with a high specific activity is not commercially available, we propose to produce and purify this enzyme from a strain of the yeast Hansenula polymorpha. This alcohol oxidase was immobilized into a gelatin matrix and its activity was estimated by a pO2 sensor. The enz...

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Veröffentlicht in:Biotechnol. Bioeng.; (United States) 1987-12, Vol.30 (9), p.1001-1005
Hauptverfasser: Belghith, H, Romette, J.L, Thomas, D
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creator Belghith, H
Romette, J.L
Thomas, D
description Since a stable alcohol oxidase with a high specific activity is not commercially available, we propose to produce and purify this enzyme from a strain of the yeast Hansenula polymorpha. This alcohol oxidase was immobilized into a gelatin matrix and its activity was estimated by a pO2 sensor. The enzyme electrode obtained was then used in a continuous flow system to measure methanol or ethanol concentrations. The sample oxygen content dependence of the signal was minimized by the support properties. Measuring time for each sample were less than two minutes including response data treatment and rinsing step. The enzyme electrode response was set for ethanol from 0.5mM to 15mM and for methanol from 10mM to 300mM. On repeated use, the electrode signal for 10mM of ethanol was stable for at least 500 assays. Analysis have been performed in different beverages such as wine and beer, and the results compared to those obtained with classical methods of analysis.
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Bioeng.; (United States)</title><addtitle>Biotechnol. Bioeng</addtitle><description>Since a stable alcohol oxidase with a high specific activity is not commercially available, we propose to produce and purify this enzyme from a strain of the yeast Hansenula polymorpha. This alcohol oxidase was immobilized into a gelatin matrix and its activity was estimated by a pO2 sensor. The enzyme electrode obtained was then used in a continuous flow system to measure methanol or ethanol concentrations. The sample oxygen content dependence of the signal was minimized by the support properties. Measuring time for each sample were less than two minutes including response data treatment and rinsing step. The enzyme electrode response was set for ethanol from 0.5mM to 15mM and for methanol from 10mM to 300mM. On repeated use, the electrode signal for 10mM of ethanol was stable for at least 500 assays. 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Psychology</topic><topic>FUNGI</topic><topic>HANSENULA</topic><topic>Hansenula polymorpha</topic><topic>HYDROXY COMPOUNDS</topic><topic>IMMOBILIZED ENZYMES</topic><topic>Industrial applications and implications. 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This alcohol oxidase was immobilized into a gelatin matrix and its activity was estimated by a pO2 sensor. The enzyme electrode obtained was then used in a continuous flow system to measure methanol or ethanol concentrations. The sample oxygen content dependence of the signal was minimized by the support properties. Measuring time for each sample were less than two minutes including response data treatment and rinsing step. The enzyme electrode response was set for ethanol from 0.5mM to 15mM and for methanol from 10mM to 300mM. On repeated use, the electrode signal for 10mM of ethanol was stable for at least 500 assays. Analysis have been performed in different beverages such as wine and beer, and the results compared to those obtained with classical methods of analysis.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18581543</pmid><doi>10.1002/bit.260300902</doi><tpages>5</tpages></addata></record>
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subjects 09 BIOMASS FUELS
090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
alcohol oxidase
ALCOHOLES
ALCOHOLS
ALCOOL
Biological and medical sciences
Biotechnology
ELECTRODES
ENZYME ACTIVITY
ENZYMES
ENZYMIC ACTIVITY
ETHANOL
FRACTIONATION
Fundamental and applied biological sciences. Psychology
FUNGI
HANSENULA
Hansenula polymorpha
HYDROXY COMPOUNDS
IMMOBILIZED ENZYMES
Industrial applications and implications. Economical aspects
METHANOL
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLANTS
PRODUCCION
PRODUCTION
SEPARATION PROCESSES
YEASTS
YIELDS
title An enzyme electrode for on-line determination of ethanol and methanol
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