AT1 and AT2 receptors in human glomerular endothelial cells at different passages
In human adult kidney angiotensin II (AngII) effects are mediated by the AT1 receptor, while the functions of AT2 receptors are mostly unknown. Since AngII regulates endothelial cell growth by AT1 and AT2 receptors, we analysed their functional aspects at different passages in human glomerular endot...
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| Veröffentlicht in: | Microvascular research 2003-07, Vol.66 (1), p.22-29 |
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| creator | Cresci, Barbara Giannini, Stefano Pala, Laura Mavilia, Carmelo Manuelli, Cinzia Cappugi, Pietro Magg, Enrico Rotella, Carlo M |
| description | In human adult kidney angiotensin II (AngII) effects are mediated by the AT1 receptor, while the functions of AT2 receptors are mostly unknown. Since AngII regulates endothelial cell growth by AT1 and AT2 receptors, we analysed their functional aspects at different passages in human glomerular endothelial cells (GENC). Semiquantitative reverse transcription-polymerase chain reaction revealed the presence of AT1 and AT2 receptors between 2p and 15p cell passages with different levels of expression. In fact, binding studies of different families of displacement curves using AngII, DUP753 (AT1 antagonist), and PD123177 (AT2 antagonist) showed the presence of AT1a and AT2 receptors at 4p–9p while in GENC 2p only the presence of AT2. In terms of mitogenic activity, AngII was unable to stimulate GENC 2p growth. On the contary, in GENC 4p–9p and 15p a significant thymidine incorporation was observed. This stimulatory effect seemed to be induced also by the concomitant release of PDGF-BB AT1a mediated. In conclusion, AT1a and AT2 receptors are represented in GENC with a different ratio depending upon the cell passage. AngII regulates the mitogenic effect through AT1a receptors (in later cell passages 4p–15p) involving the release of PDGF-BB, while AT2 (in early cell passage 2p) showed a predominant negative growth control. |
| doi_str_mv | 10.1016/S0026-2862(03)00009-8 |
| format | Article |
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Since AngII regulates endothelial cell growth by AT1 and AT2 receptors, we analysed their functional aspects at different passages in human glomerular endothelial cells (GENC). Semiquantitative reverse transcription-polymerase chain reaction revealed the presence of AT1 and AT2 receptors between 2p and 15p cell passages with different levels of expression. In fact, binding studies of different families of displacement curves using AngII, DUP753 (AT1 antagonist), and PD123177 (AT2 antagonist) showed the presence of AT1a and AT2 receptors at 4p–9p while in GENC 2p only the presence of AT2. In terms of mitogenic activity, AngII was unable to stimulate GENC 2p growth. On the contary, in GENC 4p–9p and 15p a significant thymidine incorporation was observed. This stimulatory effect seemed to be induced also by the concomitant release of PDGF-BB AT1a mediated. In conclusion, AT1a and AT2 receptors are represented in GENC with a different ratio depending upon the cell passage. AngII regulates the mitogenic effect through AT1a receptors (in later cell passages 4p–15p) involving the release of PDGF-BB, while AT2 (in early cell passage 2p) showed a predominant negative growth control.</description><identifier>ISSN: 0026-2862</identifier><identifier>EISSN: 1095-9319</identifier><identifier>DOI: 10.1016/S0026-2862(03)00009-8</identifier><identifier>PMID: 12826071</identifier><identifier>CODEN: MIVRA6</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Angiotensin II - antagonists & inhibitors ; Angiotensin II - pharmacology ; Angiotensin II receptor ; Angiotensin II Type 1 Receptor Blockers ; Angiotensin II Type 2 Receptor Blockers ; Antihypertensive Agents - pharmacology ; Biological and medical sciences ; Blotting, Western ; Cell Culture Techniques - methods ; Cell Division ; Dose-Response Relationship, Drug ; Endothelial Cells - metabolism ; Endothelium ; Fundamental and applied biological sciences. Psychology ; Growth factors ; Humans ; Imidazoles - pharmacology ; Kidney ; Kidney Glomerulus - metabolism ; Kinetics ; Losartan - pharmacology ; Platelet-Derived Growth Factor - metabolism ; Protein Binding ; Pyridines - pharmacology ; Receptor, Angiotensin, Type 1 - biosynthesis ; Receptor, Angiotensin, Type 2 - biosynthesis ; Reverse Transcriptase Polymerase Chain Reaction ; Thymidine - metabolism ; Time Factors ; Vertebrates: urinary system</subject><ispartof>Microvascular research, 2003-07, Vol.66 (1), p.22-29</ispartof><rights>2003 Elsevier Science (USA)</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-651ea83a117ca750dac8f78cd85dd51bbcca78c7ee30a55d1abaac1866f7095e3</citedby><cites>FETCH-LOGICAL-c509t-651ea83a117ca750dac8f78cd85dd51bbcca78c7ee30a55d1abaac1866f7095e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0026-2862(03)00009-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15140296$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12826071$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cresci, Barbara</creatorcontrib><creatorcontrib>Giannini, Stefano</creatorcontrib><creatorcontrib>Pala, Laura</creatorcontrib><creatorcontrib>Mavilia, Carmelo</creatorcontrib><creatorcontrib>Manuelli, Cinzia</creatorcontrib><creatorcontrib>Cappugi, Pietro</creatorcontrib><creatorcontrib>Magg, Enrico</creatorcontrib><creatorcontrib>Rotella, Carlo M</creatorcontrib><title>AT1 and AT2 receptors in human glomerular endothelial cells at different passages</title><title>Microvascular research</title><addtitle>Microvasc Res</addtitle><description>In human adult kidney angiotensin II (AngII) effects are mediated by the AT1 receptor, while the functions of AT2 receptors are mostly unknown. Since AngII regulates endothelial cell growth by AT1 and AT2 receptors, we analysed their functional aspects at different passages in human glomerular endothelial cells (GENC). Semiquantitative reverse transcription-polymerase chain reaction revealed the presence of AT1 and AT2 receptors between 2p and 15p cell passages with different levels of expression. In fact, binding studies of different families of displacement curves using AngII, DUP753 (AT1 antagonist), and PD123177 (AT2 antagonist) showed the presence of AT1a and AT2 receptors at 4p–9p while in GENC 2p only the presence of AT2. In terms of mitogenic activity, AngII was unable to stimulate GENC 2p growth. On the contary, in GENC 4p–9p and 15p a significant thymidine incorporation was observed. This stimulatory effect seemed to be induced also by the concomitant release of PDGF-BB AT1a mediated. In conclusion, AT1a and AT2 receptors are represented in GENC with a different ratio depending upon the cell passage. AngII regulates the mitogenic effect through AT1a receptors (in later cell passages 4p–15p) involving the release of PDGF-BB, while AT2 (in early cell passage 2p) showed a predominant negative growth control.</description><subject>Angiotensin II - antagonists & inhibitors</subject><subject>Angiotensin II - pharmacology</subject><subject>Angiotensin II receptor</subject><subject>Angiotensin II Type 1 Receptor Blockers</subject><subject>Angiotensin II Type 2 Receptor Blockers</subject><subject>Antihypertensive Agents - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Division</subject><subject>Dose-Response Relationship, Drug</subject><subject>Endothelial Cells - metabolism</subject><subject>Endothelium</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Imidazoles - pharmacology</subject><subject>Kidney</subject><subject>Kidney Glomerulus - metabolism</subject><subject>Kinetics</subject><subject>Losartan - pharmacology</subject><subject>Platelet-Derived Growth Factor - metabolism</subject><subject>Protein Binding</subject><subject>Pyridines - pharmacology</subject><subject>Receptor, Angiotensin, Type 1 - biosynthesis</subject><subject>Receptor, Angiotensin, Type 2 - biosynthesis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Thymidine - metabolism</subject><subject>Time Factors</subject><subject>Vertebrates: urinary system</subject><issn>0026-2862</issn><issn>1095-9319</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVISTZpfkKLLinJwemMXcnyKSxL8wELoXRzFrPSOFHxx1ayC_339X7QHDMXwfC80qtHiE8INwiov_4EyHWWG51fQXEN01SZORIzhEplVYHVsZj9R07FWUq_ABBVlZ-IU8xNrqHEmfgxX6Gkzsv5KpeRHW-GPiYZOvk6ttTJl6ZvOY4NRcmd74dXbgI10nHTJEmD9KGuOXI3yA2lRC-cPooPNTWJLw7nuXi--75aPGTLp_vHxXyZOQXVkGmFTKYgxNJRqcCTM3VpnDfKe4XrtZvWxpXMBZBSHmlN5NBoXZfTF7k4F1_2925i_3vkNNg2pG0v6rgfky2LQoNWegLVHnSxTylybTcxtBT_WgS7dWl3Lu1WlIXC7lxaM-U-Hx4Y1y37t9RB3gRcHgBKjpo6UudCeuMUfoO82ha43XM86fgTONrkAneOfZiMD9b34Z0q_wCf3ZCh</recordid><startdate>20030701</startdate><enddate>20030701</enddate><creator>Cresci, Barbara</creator><creator>Giannini, Stefano</creator><creator>Pala, Laura</creator><creator>Mavilia, Carmelo</creator><creator>Manuelli, Cinzia</creator><creator>Cappugi, Pietro</creator><creator>Magg, Enrico</creator><creator>Rotella, Carlo M</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030701</creationdate><title>AT1 and AT2 receptors in human glomerular endothelial cells at different passages</title><author>Cresci, Barbara ; Giannini, Stefano ; Pala, Laura ; Mavilia, Carmelo ; Manuelli, Cinzia ; Cappugi, Pietro ; Magg, Enrico ; Rotella, Carlo M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-651ea83a117ca750dac8f78cd85dd51bbcca78c7ee30a55d1abaac1866f7095e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Angiotensin II - antagonists & inhibitors</topic><topic>Angiotensin II - pharmacology</topic><topic>Angiotensin II receptor</topic><topic>Angiotensin II Type 1 Receptor Blockers</topic><topic>Angiotensin II Type 2 Receptor Blockers</topic><topic>Antihypertensive Agents - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Division</topic><topic>Dose-Response Relationship, Drug</topic><topic>Endothelial Cells - metabolism</topic><topic>Endothelium</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Imidazoles - pharmacology</topic><topic>Kidney</topic><topic>Kidney Glomerulus - metabolism</topic><topic>Kinetics</topic><topic>Losartan - pharmacology</topic><topic>Platelet-Derived Growth Factor - metabolism</topic><topic>Protein Binding</topic><topic>Pyridines - pharmacology</topic><topic>Receptor, Angiotensin, Type 1 - biosynthesis</topic><topic>Receptor, Angiotensin, Type 2 - biosynthesis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Thymidine - metabolism</topic><topic>Time Factors</topic><topic>Vertebrates: urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cresci, Barbara</creatorcontrib><creatorcontrib>Giannini, Stefano</creatorcontrib><creatorcontrib>Pala, Laura</creatorcontrib><creatorcontrib>Mavilia, Carmelo</creatorcontrib><creatorcontrib>Manuelli, Cinzia</creatorcontrib><creatorcontrib>Cappugi, Pietro</creatorcontrib><creatorcontrib>Magg, Enrico</creatorcontrib><creatorcontrib>Rotella, Carlo M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microvascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cresci, Barbara</au><au>Giannini, Stefano</au><au>Pala, Laura</au><au>Mavilia, Carmelo</au><au>Manuelli, Cinzia</au><au>Cappugi, Pietro</au><au>Magg, Enrico</au><au>Rotella, Carlo M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AT1 and AT2 receptors in human glomerular endothelial cells at different passages</atitle><jtitle>Microvascular research</jtitle><addtitle>Microvasc Res</addtitle><date>2003-07-01</date><risdate>2003</risdate><volume>66</volume><issue>1</issue><spage>22</spage><epage>29</epage><pages>22-29</pages><issn>0026-2862</issn><eissn>1095-9319</eissn><coden>MIVRA6</coden><abstract>In human adult kidney angiotensin II (AngII) effects are mediated by the AT1 receptor, while the functions of AT2 receptors are mostly unknown. Since AngII regulates endothelial cell growth by AT1 and AT2 receptors, we analysed their functional aspects at different passages in human glomerular endothelial cells (GENC). Semiquantitative reverse transcription-polymerase chain reaction revealed the presence of AT1 and AT2 receptors between 2p and 15p cell passages with different levels of expression. In fact, binding studies of different families of displacement curves using AngII, DUP753 (AT1 antagonist), and PD123177 (AT2 antagonist) showed the presence of AT1a and AT2 receptors at 4p–9p while in GENC 2p only the presence of AT2. In terms of mitogenic activity, AngII was unable to stimulate GENC 2p growth. On the contary, in GENC 4p–9p and 15p a significant thymidine incorporation was observed. This stimulatory effect seemed to be induced also by the concomitant release of PDGF-BB AT1a mediated. In conclusion, AT1a and AT2 receptors are represented in GENC with a different ratio depending upon the cell passage. AngII regulates the mitogenic effect through AT1a receptors (in later cell passages 4p–15p) involving the release of PDGF-BB, while AT2 (in early cell passage 2p) showed a predominant negative growth control.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>12826071</pmid><doi>10.1016/S0026-2862(03)00009-8</doi><tpages>8</tpages></addata></record> |
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| subjects | Angiotensin II - antagonists & inhibitors Angiotensin II - pharmacology Angiotensin II receptor Angiotensin II Type 1 Receptor Blockers Angiotensin II Type 2 Receptor Blockers Antihypertensive Agents - pharmacology Biological and medical sciences Blotting, Western Cell Culture Techniques - methods Cell Division Dose-Response Relationship, Drug Endothelial Cells - metabolism Endothelium Fundamental and applied biological sciences. Psychology Growth factors Humans Imidazoles - pharmacology Kidney Kidney Glomerulus - metabolism Kinetics Losartan - pharmacology Platelet-Derived Growth Factor - metabolism Protein Binding Pyridines - pharmacology Receptor, Angiotensin, Type 1 - biosynthesis Receptor, Angiotensin, Type 2 - biosynthesis Reverse Transcriptase Polymerase Chain Reaction Thymidine - metabolism Time Factors Vertebrates: urinary system |
| title | AT1 and AT2 receptors in human glomerular endothelial cells at different passages |
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