Detection and characterization of extended-spectrum beta-lactamase (TEM-52)-producing Salmonella serotype Infantis from broilers in Japan
During 2004 and 2006, multidrug-resistant Salmonella enterica subspecies enterica serovar Infantis (Salmonella Infantis) isolates (n = 120) were recovered from broiler cecal samples collected from a meat-processing plant, and the isolates were examined. The study was conducted to detect and characte...
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| Veröffentlicht in: | Foodborne pathogens and disease 2010-05, Vol.7 (5), p.515-521 |
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| creator | Shahada, Francis Chuma, Takehisa Dahshan, Hesham Akiba, Masato Sueyoshi, Masuo Okamoto, Karoku |
| description | During 2004 and 2006, multidrug-resistant Salmonella enterica subspecies enterica serovar Infantis (Salmonella Infantis) isolates (n = 120) were recovered from broiler cecal samples collected from a meat-processing plant, and the isolates were examined. The study was conducted to detect and characterize extended-spectrum beta-lactamase (ESBL)-producing Salmonella Infantis isolates recovered from broiler chickens and determine the mechanisms of transfer of the resistance traits. Extended-spectrum cephalosporins-resistant Salmonella Infantis isolates producing ESBL TEM-52 were detected. The mutant bla(TEM-52) gene and the wild-type bla(TEM-1) gene that mediated resistance to ampicillin (an extended-spectrum penicillin) and cephalothin (a narrow-spectrum cephalosporin) were located on approximately 50-kb conjugative plasmids among beta-lactam-resistant (n = 29) isolates. The bla(TEM) genes did not cotransfer with aadA1, sul1 (both associated with class 1 integrons), tetA, and dfrA5, signifying a chromosomal location of these non-beta-lactam resistance-encoding genes. This is the first report describing TEM-52-producing S. enterica from food-producing animals in Japan. An emergence of TEM-type ESBL is an important concern to public health because this readily transferable resistance mechanism threatens the value of the third-generation cephalosporins and may reduce the clinical utility of this class of antibiotics against pathogenic Gram-negative bacteria. |
| doi_str_mv | 10.1089/fpd.2009.0454 |
| format | Article |
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The study was conducted to detect and characterize extended-spectrum beta-lactamase (ESBL)-producing Salmonella Infantis isolates recovered from broiler chickens and determine the mechanisms of transfer of the resistance traits. Extended-spectrum cephalosporins-resistant Salmonella Infantis isolates producing ESBL TEM-52 were detected. The mutant bla(TEM-52) gene and the wild-type bla(TEM-1) gene that mediated resistance to ampicillin (an extended-spectrum penicillin) and cephalothin (a narrow-spectrum cephalosporin) were located on approximately 50-kb conjugative plasmids among beta-lactam-resistant (n = 29) isolates. The bla(TEM) genes did not cotransfer with aadA1, sul1 (both associated with class 1 integrons), tetA, and dfrA5, signifying a chromosomal location of these non-beta-lactam resistance-encoding genes. This is the first report describing TEM-52-producing S. enterica from food-producing animals in Japan. An emergence of TEM-type ESBL is an important concern to public health because this readily transferable resistance mechanism threatens the value of the third-generation cephalosporins and may reduce the clinical utility of this class of antibiotics against pathogenic Gram-negative bacteria.</description><identifier>ISSN: 1535-3141</identifier><identifier>EISSN: 1556-7125</identifier><identifier>DOI: 10.1089/fpd.2009.0454</identifier><identifier>PMID: 20039796</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Ampicillin Resistance - genetics ; Animals ; Beta lactamases ; beta-Lactamases - biosynthesis ; beta-Lactamases - chemistry ; beta-Lactamases - genetics ; Cecum - microbiology ; Cephalosporin Resistance - genetics ; Chickens - microbiology ; Diagnosis ; Drug Resistance, Multiple, Bacterial - genetics ; Drug Synergism ; Food Microbiology ; Gene Transfer, Horizontal ; Genetic aspects ; Genotype ; Integrons - genetics ; Japan ; Microbial Sensitivity Tests ; Phenotype ; Physiological aspects ; Plasmids - genetics ; Salmonella ; Salmonella enterica - drug effects ; Salmonella enterica - enzymology ; Salmonella enterica - genetics ; Salmonella enterica - isolation & purification ; Salmonella food poisoning ; Serotyping</subject><ispartof>Foodborne pathogens and disease, 2010-05, Vol.7 (5), p.515-521</ispartof><rights>COPYRIGHT 2010 Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-a33ab228c21685c9a844d3c18be768bed60c54c493cb644eff3ff1ddb040affe3</citedby><cites>FETCH-LOGICAL-c359t-a33ab228c21685c9a844d3c18be768bed60c54c493cb644eff3ff1ddb040affe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20039796$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shahada, Francis</creatorcontrib><creatorcontrib>Chuma, Takehisa</creatorcontrib><creatorcontrib>Dahshan, Hesham</creatorcontrib><creatorcontrib>Akiba, Masato</creatorcontrib><creatorcontrib>Sueyoshi, Masuo</creatorcontrib><creatorcontrib>Okamoto, Karoku</creatorcontrib><title>Detection and characterization of extended-spectrum beta-lactamase (TEM-52)-producing Salmonella serotype Infantis from broilers in Japan</title><title>Foodborne pathogens and disease</title><addtitle>Foodborne Pathog Dis</addtitle><description>During 2004 and 2006, multidrug-resistant Salmonella enterica subspecies enterica serovar Infantis (Salmonella Infantis) isolates (n = 120) were recovered from broiler cecal samples collected from a meat-processing plant, and the isolates were examined. The study was conducted to detect and characterize extended-spectrum beta-lactamase (ESBL)-producing Salmonella Infantis isolates recovered from broiler chickens and determine the mechanisms of transfer of the resistance traits. Extended-spectrum cephalosporins-resistant Salmonella Infantis isolates producing ESBL TEM-52 were detected. The mutant bla(TEM-52) gene and the wild-type bla(TEM-1) gene that mediated resistance to ampicillin (an extended-spectrum penicillin) and cephalothin (a narrow-spectrum cephalosporin) were located on approximately 50-kb conjugative plasmids among beta-lactam-resistant (n = 29) isolates. The bla(TEM) genes did not cotransfer with aadA1, sul1 (both associated with class 1 integrons), tetA, and dfrA5, signifying a chromosomal location of these non-beta-lactam resistance-encoding genes. This is the first report describing TEM-52-producing S. enterica from food-producing animals in Japan. An emergence of TEM-type ESBL is an important concern to public health because this readily transferable resistance mechanism threatens the value of the third-generation cephalosporins and may reduce the clinical utility of this class of antibiotics against pathogenic Gram-negative bacteria.</description><subject>Ampicillin Resistance - genetics</subject><subject>Animals</subject><subject>Beta lactamases</subject><subject>beta-Lactamases - biosynthesis</subject><subject>beta-Lactamases - chemistry</subject><subject>beta-Lactamases - genetics</subject><subject>Cecum - microbiology</subject><subject>Cephalosporin Resistance - genetics</subject><subject>Chickens - microbiology</subject><subject>Diagnosis</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>Drug Synergism</subject><subject>Food Microbiology</subject><subject>Gene Transfer, Horizontal</subject><subject>Genetic aspects</subject><subject>Genotype</subject><subject>Integrons - genetics</subject><subject>Japan</subject><subject>Microbial Sensitivity Tests</subject><subject>Phenotype</subject><subject>Physiological aspects</subject><subject>Plasmids - genetics</subject><subject>Salmonella</subject><subject>Salmonella enterica - drug effects</subject><subject>Salmonella enterica - enzymology</subject><subject>Salmonella enterica - genetics</subject><subject>Salmonella enterica - isolation & purification</subject><subject>Salmonella food poisoning</subject><subject>Serotyping</subject><issn>1535-3141</issn><issn>1556-7125</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU9vFSEUxSdGY2t16daQuKguePJ35rFsatWaGhfWNWHgUjEzMAKTWL-B31rGV01MDAkQ8jv3Hu7puqeU7CjZq1d-cTtGiNoRIcW97phK2eOBMnl_u3OJORX0qHtUyldCmGJyeNgdNQFXg-qPu5-voYKtIUVkokP2i8nGVsjhh_n9mDyC7xWiA4fL0si8zmiEavDUODObAujF9cUHLNlLvOTkVhviDfpkpjlFmCaDCuRUbxdAl9GbWENBPqdWI6cwQS4oRPTeLCY-7h54MxV4cneedJ_fXFyfv8NXH99enp9dYculqthwbkbG9pbRfi-tMnshHLd0P8LQt831xEphheJ27IUA77n31LmRCGK8B37SnR7qNrffVihVz6HYzWqEtBY98NZHMCUa-fxA3pgJdIg-1TacjdZnjA1sUIT3jdr9h2rLwRxsG4Jv__xXgA8Cm1MpGbxecphNvtWU6C1T3TLVW6Z6y7Txz-4Mr-MM7i_9J0T-CyBoniU</recordid><startdate>201005</startdate><enddate>201005</enddate><creator>Shahada, Francis</creator><creator>Chuma, Takehisa</creator><creator>Dahshan, Hesham</creator><creator>Akiba, Masato</creator><creator>Sueyoshi, Masuo</creator><creator>Okamoto, Karoku</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201005</creationdate><title>Detection and characterization of extended-spectrum beta-lactamase (TEM-52)-producing Salmonella serotype Infantis from broilers in Japan</title><author>Shahada, Francis ; Chuma, Takehisa ; Dahshan, Hesham ; Akiba, Masato ; Sueyoshi, Masuo ; Okamoto, Karoku</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-a33ab228c21685c9a844d3c18be768bed60c54c493cb644eff3ff1ddb040affe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Ampicillin Resistance - genetics</topic><topic>Animals</topic><topic>Beta lactamases</topic><topic>beta-Lactamases - biosynthesis</topic><topic>beta-Lactamases - chemistry</topic><topic>beta-Lactamases - genetics</topic><topic>Cecum - microbiology</topic><topic>Cephalosporin Resistance - genetics</topic><topic>Chickens - microbiology</topic><topic>Diagnosis</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>Drug Synergism</topic><topic>Food Microbiology</topic><topic>Gene Transfer, Horizontal</topic><topic>Genetic aspects</topic><topic>Genotype</topic><topic>Integrons - genetics</topic><topic>Japan</topic><topic>Microbial Sensitivity Tests</topic><topic>Phenotype</topic><topic>Physiological aspects</topic><topic>Plasmids - genetics</topic><topic>Salmonella</topic><topic>Salmonella enterica - drug effects</topic><topic>Salmonella enterica - enzymology</topic><topic>Salmonella enterica - genetics</topic><topic>Salmonella enterica - isolation & purification</topic><topic>Salmonella food poisoning</topic><topic>Serotyping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shahada, Francis</creatorcontrib><creatorcontrib>Chuma, Takehisa</creatorcontrib><creatorcontrib>Dahshan, Hesham</creatorcontrib><creatorcontrib>Akiba, Masato</creatorcontrib><creatorcontrib>Sueyoshi, Masuo</creatorcontrib><creatorcontrib>Okamoto, Karoku</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Foodborne pathogens and disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shahada, Francis</au><au>Chuma, Takehisa</au><au>Dahshan, Hesham</au><au>Akiba, Masato</au><au>Sueyoshi, Masuo</au><au>Okamoto, Karoku</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and characterization of extended-spectrum beta-lactamase (TEM-52)-producing Salmonella serotype Infantis from broilers in Japan</atitle><jtitle>Foodborne pathogens and disease</jtitle><addtitle>Foodborne Pathog Dis</addtitle><date>2010-05</date><risdate>2010</risdate><volume>7</volume><issue>5</issue><spage>515</spage><epage>521</epage><pages>515-521</pages><issn>1535-3141</issn><eissn>1556-7125</eissn><abstract>During 2004 and 2006, multidrug-resistant Salmonella enterica subspecies enterica serovar Infantis (Salmonella Infantis) isolates (n = 120) were recovered from broiler cecal samples collected from a meat-processing plant, and the isolates were examined. The study was conducted to detect and characterize extended-spectrum beta-lactamase (ESBL)-producing Salmonella Infantis isolates recovered from broiler chickens and determine the mechanisms of transfer of the resistance traits. Extended-spectrum cephalosporins-resistant Salmonella Infantis isolates producing ESBL TEM-52 were detected. The mutant bla(TEM-52) gene and the wild-type bla(TEM-1) gene that mediated resistance to ampicillin (an extended-spectrum penicillin) and cephalothin (a narrow-spectrum cephalosporin) were located on approximately 50-kb conjugative plasmids among beta-lactam-resistant (n = 29) isolates. The bla(TEM) genes did not cotransfer with aadA1, sul1 (both associated with class 1 integrons), tetA, and dfrA5, signifying a chromosomal location of these non-beta-lactam resistance-encoding genes. This is the first report describing TEM-52-producing S. enterica from food-producing animals in Japan. 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| subjects | Ampicillin Resistance - genetics Animals Beta lactamases beta-Lactamases - biosynthesis beta-Lactamases - chemistry beta-Lactamases - genetics Cecum - microbiology Cephalosporin Resistance - genetics Chickens - microbiology Diagnosis Drug Resistance, Multiple, Bacterial - genetics Drug Synergism Food Microbiology Gene Transfer, Horizontal Genetic aspects Genotype Integrons - genetics Japan Microbial Sensitivity Tests Phenotype Physiological aspects Plasmids - genetics Salmonella Salmonella enterica - drug effects Salmonella enterica - enzymology Salmonella enterica - genetics Salmonella enterica - isolation & purification Salmonella food poisoning Serotyping |
| title | Detection and characterization of extended-spectrum beta-lactamase (TEM-52)-producing Salmonella serotype Infantis from broilers in Japan |
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