A reliable and simplified sj/β-TREC ratio quantification method for human thymic output measurement

Current techniques to peripherally assess thymic function are: the signal-joint T-cell receptor excision circle (sj-TREC) level measurement and the naive T cell and CD31+ TREC-rich subset determination. However, all of them are indirect approaches and none could be considered a direct recent thymic...

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Veröffentlicht in:	Journal of immunological methods 2010-01, Vol.352 (1), p.111-117
Hauptverfasser:	Ferrando-Martínez, Sara, Franco, Jaime M., Ruiz-Mateos, Ezequiel, Hernández, Ana, Ordoñez, Antonio, Gutierrez, Encarnación, Leal, Manuel
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, CD - biosynthesis Antigens, Differentiation - genetics Antigens, Differentiation - immunology Biological and medical sciences Cell Differentiation Cell Proliferation DβJβ-TREC Female Fetal Blood - cytology Fundamental and applied biological sciences. Psychology Fundamental immunology Gene Rearrangement - genetics Gene Rearrangement - immunology HIV Human thymic function Humans Intrathymic proliferation Microbiology Molecular immunology Polymerase Chain Reaction Receptors, Antigen, T-Cell - genetics Receptors, Antigen, T-Cell - immunology Receptors, Antigen, T-Cell - metabolism Reproducibility of Results Sj-TREC Techniques Techniques used in virology Thymus Gland - cytology Thymus Gland - growth & development Thymus Gland - immunology Thymus Gland - metabolism TREC ratio Virology
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