Expression profile of cathepsin B in the fat body of Bombyx mori during metamorphosis
Proteolytic enzymes are involved in insect molting and metamorphosis, and play a vital role in the programmed cell death of obsolete organs. Here we show the expression profile of cathepsin B in the fat body of the silkworm Bombyx mori during development. We also compare the expression profiles of B...
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| Veröffentlicht in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2009-10, Vol.154 (2), p.188-194 |
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| container_title | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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| creator | Lee, Kwang Sik Kim, Bo Yeon Choo, Young Moo Yoon, Hyung Joo Kang, Pil Don Woo, Soo Dong Sohn, Hung Dae Roh, Jong Yul Gui, Zhong Zheng Je, Yeon Ho Jin, Byung Rae |
| description | Proteolytic enzymes are involved in insect molting and metamorphosis, and play a vital role in the programmed cell death of obsolete organs. Here we show the expression profile of cathepsin B in the fat body of the silkworm
Bombyx mori during development. We also compare the expression profiles of
B. mori cathepsins B (
BmCatB) and D (
BmCatD) during normal development and after RNA interference (RNAi)-mediated inhibition.
BmCatB is induced by 20-OH-ecdysone, and is expressed in the fat body of
B. mori during molting and the larval–pupal and pupal–adult transformations, where its expression leads to programmed cell death. In particular,
BmCatB is highly expressed in the fat body of
B. mori during the larval–pupal transformation, and
BmCatB RNAi treatment resulted in an arrest of the larval–pupal transformation. RNAi-mediated
BmCatB knockdown sustained the expression of
BmCatD during the larval–pupal transformation. On the other hand, when
BmCatD was inhibited via RNAi, the expression of
BmCatB was upregulated. Based on these results, we conclude that
BmCatB is involved in the programmed cell death of the fat body during
B. mori metamorphosis, and that
BmCatB and
BmCatD contribute to
B. mori metamorphosis. |
| doi_str_mv | 10.1016/j.cbpb.2009.06.002 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733572157</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1096495909001596</els_id><sourcerecordid>20800350</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-d740eec52a32a8cbf1b540daf4c3ab1467cd776aa04d9cf02dc8b2d07e37df953</originalsourceid><addsrcrecordid>eNp9kE1P3DAQhi1EVSj0D_RQ-URPCWM7iROJS0EUkJB6gbPljzF4tYmDnUXsv69Xu1JvXDwezTOv7IeQHwxqBqy7XNXWzKbmAEMNXQ3Aj8gp6-VQMQbyuNxh6KpmaIcT8i3nFYDomWBfyQkbWjFI2ZyS59uPOWHOIU50TtGHNdLoqdXLK845TPSalqM01OuFmui2u_F1HM32g44xBeo2KUwvdMRFl35-jTnkc_LF63XG74d6Rp7_3D7d3FePf-8ebn4_Vlb03VI52QCibbkWXPfWeGbaBpz2jRXasKaT1knZaQ2NG6wH7mxvuAOJQjpf_nBGfu1zy9PfNpgXNYZscb3WE8ZNVlKIVnLWykJefEpy6IueFgrI96BNMeeEXs0pjDptFQO1065Waqdd7bQr6FTRXpZ-HtI3ZkT3f-XguQBXewCLjfeASWUbcLLoQkK7KBfDZ_n_AMz3lGg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20800350</pqid></control><display><type>article</type><title>Expression profile of cathepsin B in the fat body of Bombyx mori during metamorphosis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Lee, Kwang Sik ; Kim, Bo Yeon ; Choo, Young Moo ; Yoon, Hyung Joo ; Kang, Pil Don ; Woo, Soo Dong ; Sohn, Hung Dae ; Roh, Jong Yul ; Gui, Zhong Zheng ; Je, Yeon Ho ; Jin, Byung Rae</creator><creatorcontrib>Lee, Kwang Sik ; Kim, Bo Yeon ; Choo, Young Moo ; Yoon, Hyung Joo ; Kang, Pil Don ; Woo, Soo Dong ; Sohn, Hung Dae ; Roh, Jong Yul ; Gui, Zhong Zheng ; Je, Yeon Ho ; Jin, Byung Rae</creatorcontrib><description>Proteolytic enzymes are involved in insect molting and metamorphosis, and play a vital role in the programmed cell death of obsolete organs. Here we show the expression profile of cathepsin B in the fat body of the silkworm
Bombyx mori during development. We also compare the expression profiles of
B. mori cathepsins B (
BmCatB) and D (
BmCatD) during normal development and after RNA interference (RNAi)-mediated inhibition.
BmCatB is induced by 20-OH-ecdysone, and is expressed in the fat body of
B. mori during molting and the larval–pupal and pupal–adult transformations, where its expression leads to programmed cell death. In particular,
BmCatB is highly expressed in the fat body of
B. mori during the larval–pupal transformation, and
BmCatB RNAi treatment resulted in an arrest of the larval–pupal transformation. RNAi-mediated
BmCatB knockdown sustained the expression of
BmCatD during the larval–pupal transformation. On the other hand, when
BmCatD was inhibited via RNAi, the expression of
BmCatB was upregulated. Based on these results, we conclude that
BmCatB is involved in the programmed cell death of the fat body during
B. mori metamorphosis, and that
BmCatB and
BmCatD contribute to
B. mori metamorphosis.</description><identifier>ISSN: 1096-4959</identifier><identifier>EISSN: 1879-1107</identifier><identifier>DOI: 10.1016/j.cbpb.2009.06.002</identifier><identifier>PMID: 19539774</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Animals ; Apoptosis ; Apoptosis - drug effects ; Apoptosis - genetics ; Bombyx - drug effects ; Bombyx - genetics ; Bombyx - growth & development ; Bombyx mori ; Cathepsin ; Cathepsin B ; Cathepsin B - deficiency ; Cathepsin B - genetics ; Cathepsin B - metabolism ; Cathepsin D - deficiency ; Cathepsin D - genetics ; Cathepsin D - metabolism ; Ecdysterone - pharmacology ; Fat Body - drug effects ; Fat Body - metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Developmental - drug effects ; Gene Knockdown Techniques ; Insects ; Metamorphosis ; Metamorphosis, Biological - genetics ; Molt ; Proteolysis ; RNA Interference ; RNAi ; Silk worm</subject><ispartof>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2009-10, Vol.154 (2), p.188-194</ispartof><rights>2009 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-d740eec52a32a8cbf1b540daf4c3ab1467cd776aa04d9cf02dc8b2d07e37df953</citedby><cites>FETCH-LOGICAL-c386t-d740eec52a32a8cbf1b540daf4c3ab1467cd776aa04d9cf02dc8b2d07e37df953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cbpb.2009.06.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19539774$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Kwang Sik</creatorcontrib><creatorcontrib>Kim, Bo Yeon</creatorcontrib><creatorcontrib>Choo, Young Moo</creatorcontrib><creatorcontrib>Yoon, Hyung Joo</creatorcontrib><creatorcontrib>Kang, Pil Don</creatorcontrib><creatorcontrib>Woo, Soo Dong</creatorcontrib><creatorcontrib>Sohn, Hung Dae</creatorcontrib><creatorcontrib>Roh, Jong Yul</creatorcontrib><creatorcontrib>Gui, Zhong Zheng</creatorcontrib><creatorcontrib>Je, Yeon Ho</creatorcontrib><creatorcontrib>Jin, Byung Rae</creatorcontrib><title>Expression profile of cathepsin B in the fat body of Bombyx mori during metamorphosis</title><title>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</title><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><description>Proteolytic enzymes are involved in insect molting and metamorphosis, and play a vital role in the programmed cell death of obsolete organs. Here we show the expression profile of cathepsin B in the fat body of the silkworm
Bombyx mori during development. We also compare the expression profiles of
B. mori cathepsins B (
BmCatB) and D (
BmCatD) during normal development and after RNA interference (RNAi)-mediated inhibition.
BmCatB is induced by 20-OH-ecdysone, and is expressed in the fat body of
B. mori during molting and the larval–pupal and pupal–adult transformations, where its expression leads to programmed cell death. In particular,
BmCatB is highly expressed in the fat body of
B. mori during the larval–pupal transformation, and
BmCatB RNAi treatment resulted in an arrest of the larval–pupal transformation. RNAi-mediated
BmCatB knockdown sustained the expression of
BmCatD during the larval–pupal transformation. On the other hand, when
BmCatD was inhibited via RNAi, the expression of
BmCatB was upregulated. Based on these results, we conclude that
BmCatB is involved in the programmed cell death of the fat body during
B. mori metamorphosis, and that
BmCatB and
BmCatD contribute to
B. mori metamorphosis.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - genetics</subject><subject>Bombyx - drug effects</subject><subject>Bombyx - genetics</subject><subject>Bombyx - growth & development</subject><subject>Bombyx mori</subject><subject>Cathepsin</subject><subject>Cathepsin B</subject><subject>Cathepsin B - deficiency</subject><subject>Cathepsin B - genetics</subject><subject>Cathepsin B - metabolism</subject><subject>Cathepsin D - deficiency</subject><subject>Cathepsin D - genetics</subject><subject>Cathepsin D - metabolism</subject><subject>Ecdysterone - pharmacology</subject><subject>Fat Body - drug effects</subject><subject>Fat Body - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Gene Knockdown Techniques</subject><subject>Insects</subject><subject>Metamorphosis</subject><subject>Metamorphosis, Biological - genetics</subject><subject>Molt</subject><subject>Proteolysis</subject><subject>RNA Interference</subject><subject>RNAi</subject><subject>Silk worm</subject><issn>1096-4959</issn><issn>1879-1107</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1P3DAQhi1EVSj0D_RQ-URPCWM7iROJS0EUkJB6gbPljzF4tYmDnUXsv69Xu1JvXDwezTOv7IeQHwxqBqy7XNXWzKbmAEMNXQ3Aj8gp6-VQMQbyuNxh6KpmaIcT8i3nFYDomWBfyQkbWjFI2ZyS59uPOWHOIU50TtGHNdLoqdXLK845TPSalqM01OuFmui2u_F1HM32g44xBeo2KUwvdMRFl35-jTnkc_LF63XG74d6Rp7_3D7d3FePf-8ebn4_Vlb03VI52QCibbkWXPfWeGbaBpz2jRXasKaT1knZaQ2NG6wH7mxvuAOJQjpf_nBGfu1zy9PfNpgXNYZscb3WE8ZNVlKIVnLWykJefEpy6IueFgrI96BNMeeEXs0pjDptFQO1065Waqdd7bQr6FTRXpZ-HtI3ZkT3f-XguQBXewCLjfeASWUbcLLoQkK7KBfDZ_n_AMz3lGg</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Lee, Kwang Sik</creator><creator>Kim, Bo Yeon</creator><creator>Choo, Young Moo</creator><creator>Yoon, Hyung Joo</creator><creator>Kang, Pil Don</creator><creator>Woo, Soo Dong</creator><creator>Sohn, Hung Dae</creator><creator>Roh, Jong Yul</creator><creator>Gui, Zhong Zheng</creator><creator>Je, Yeon Ho</creator><creator>Jin, Byung Rae</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20091001</creationdate><title>Expression profile of cathepsin B in the fat body of Bombyx mori during metamorphosis</title><author>Lee, Kwang Sik ; Kim, Bo Yeon ; Choo, Young Moo ; Yoon, Hyung Joo ; Kang, Pil Don ; Woo, Soo Dong ; Sohn, Hung Dae ; Roh, Jong Yul ; Gui, Zhong Zheng ; Je, Yeon Ho ; Jin, Byung Rae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-d740eec52a32a8cbf1b540daf4c3ab1467cd776aa04d9cf02dc8b2d07e37df953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - genetics</topic><topic>Bombyx - drug effects</topic><topic>Bombyx - genetics</topic><topic>Bombyx - growth & development</topic><topic>Bombyx mori</topic><topic>Cathepsin</topic><topic>Cathepsin B</topic><topic>Cathepsin B - deficiency</topic><topic>Cathepsin B - genetics</topic><topic>Cathepsin B - metabolism</topic><topic>Cathepsin D - deficiency</topic><topic>Cathepsin D - genetics</topic><topic>Cathepsin D - metabolism</topic><topic>Ecdysterone - pharmacology</topic><topic>Fat Body - drug effects</topic><topic>Fat Body - metabolism</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Gene Knockdown Techniques</topic><topic>Insects</topic><topic>Metamorphosis</topic><topic>Metamorphosis, Biological - genetics</topic><topic>Molt</topic><topic>Proteolysis</topic><topic>RNA Interference</topic><topic>RNAi</topic><topic>Silk worm</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Kwang Sik</creatorcontrib><creatorcontrib>Kim, Bo Yeon</creatorcontrib><creatorcontrib>Choo, Young Moo</creatorcontrib><creatorcontrib>Yoon, Hyung Joo</creatorcontrib><creatorcontrib>Kang, Pil Don</creatorcontrib><creatorcontrib>Woo, Soo Dong</creatorcontrib><creatorcontrib>Sohn, Hung Dae</creatorcontrib><creatorcontrib>Roh, Jong Yul</creatorcontrib><creatorcontrib>Gui, Zhong Zheng</creatorcontrib><creatorcontrib>Je, Yeon Ho</creatorcontrib><creatorcontrib>Jin, Byung Rae</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Kwang Sik</au><au>Kim, Bo Yeon</au><au>Choo, Young Moo</au><au>Yoon, Hyung Joo</au><au>Kang, Pil Don</au><au>Woo, Soo Dong</au><au>Sohn, Hung Dae</au><au>Roh, Jong Yul</au><au>Gui, Zhong Zheng</au><au>Je, Yeon Ho</au><au>Jin, Byung Rae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression profile of cathepsin B in the fat body of Bombyx mori during metamorphosis</atitle><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>154</volume><issue>2</issue><spage>188</spage><epage>194</epage><pages>188-194</pages><issn>1096-4959</issn><eissn>1879-1107</eissn><abstract>Proteolytic enzymes are involved in insect molting and metamorphosis, and play a vital role in the programmed cell death of obsolete organs. Here we show the expression profile of cathepsin B in the fat body of the silkworm
Bombyx mori during development. We also compare the expression profiles of
B. mori cathepsins B (
BmCatB) and D (
BmCatD) during normal development and after RNA interference (RNAi)-mediated inhibition.
BmCatB is induced by 20-OH-ecdysone, and is expressed in the fat body of
B. mori during molting and the larval–pupal and pupal–adult transformations, where its expression leads to programmed cell death. In particular,
BmCatB is highly expressed in the fat body of
B. mori during the larval–pupal transformation, and
BmCatB RNAi treatment resulted in an arrest of the larval–pupal transformation. RNAi-mediated
BmCatB knockdown sustained the expression of
BmCatD during the larval–pupal transformation. On the other hand, when
BmCatD was inhibited via RNAi, the expression of
BmCatB was upregulated. Based on these results, we conclude that
BmCatB is involved in the programmed cell death of the fat body during
B. mori metamorphosis, and that
BmCatB and
BmCatD contribute to
B. mori metamorphosis.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>19539774</pmid><doi>10.1016/j.cbpb.2009.06.002</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1096-4959 |
| ispartof | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2009-10, Vol.154 (2), p.188-194 |
| issn | 1096-4959 1879-1107 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Apoptosis Apoptosis - drug effects Apoptosis - genetics Bombyx - drug effects Bombyx - genetics Bombyx - growth & development Bombyx mori Cathepsin Cathepsin B Cathepsin B - deficiency Cathepsin B - genetics Cathepsin B - metabolism Cathepsin D - deficiency Cathepsin D - genetics Cathepsin D - metabolism Ecdysterone - pharmacology Fat Body - drug effects Fat Body - metabolism Gene Expression Profiling Gene Expression Regulation, Developmental - drug effects Gene Knockdown Techniques Insects Metamorphosis Metamorphosis, Biological - genetics Molt Proteolysis RNA Interference RNAi Silk worm |
| title | Expression profile of cathepsin B in the fat body of Bombyx mori during metamorphosis |
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