Automated magnetic preparation of DNA templates for solid phase sequencing

An integrated protocol for solid‐phase DNA sequencing using a robotic work station is described involving magnetic separation of DNA and analysis of the sequencing product by electrophoresis with automated detection of the fluorescently labeled fragments. The method, which is based on magnetic beads...

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Veröffentlicht in:	Electrophoresis 1992, Vol.13 (1), p.547-551
Hauptverfasser:	Wahlberg, Johan, Holmberg, Anders, Bergh, Staffan, Hultman, Thomas, Uhlén, Mathias
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Analytical, structural and metabolic biochemistry Automation Base Sequence Biological and medical sciences DNA - chemistry DNA, Single-Stranded - isolation & purification Electrophoresis Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Magnetics Microspheres Molecular Sequence Data Nucleic acids Oligonucleotides - chemical synthesis Plasmids - genetics Polymerase Chain Reaction Robotics Templates, Genetic
Online-Zugang:	Volltext
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