Na(+):aspartate coupling stoichiometry in the glutamate transporter homologue Glt(Ph)
The Na(+) aspartate symporter Glt(Ph) from Pyrococcus horikoshii is the only member of the glutamate transporter family for which crystal structures have been determined. The cation:aspartate coupling stoichiometry is unknown, thus hampering the elucidation of the ion coupling mechanism. Here we mea...
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| Veröffentlicht in: | Biochemistry (Easton) 2010-05, Vol.49 (17), p.3511-3513 |
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| container_issue | 17 |
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| container_title | Biochemistry (Easton) |
| container_volume | 49 |
| creator | Groeneveld, Maarten Slotboom, Dirk-Jan |
| description | The Na(+) aspartate symporter Glt(Ph) from Pyrococcus horikoshii is the only member of the glutamate transporter family for which crystal structures have been determined. The cation:aspartate coupling stoichiometry is unknown, thus hampering the elucidation of the ion coupling mechanism. Here we measure transport of (22)Na(+) and [(14)C]aspartate in proteoliposomes containing purified Glt(Ph) and demonstrate that three Na(+) ions are symported with aspartate. |
| doi_str_mv | 10.1021/bi100430s |
| format | Article |
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The cation:aspartate coupling stoichiometry is unknown, thus hampering the elucidation of the ion coupling mechanism. 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| fulltext | fulltext |
| identifier | EISSN: 1520-4995 |
| ispartof | Biochemistry (Easton), 2010-05, Vol.49 (17), p.3511-3513 |
| issn | 1520-4995 |
| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Amino Acid Transport System X-AG - chemistry Aspartic Acid - chemistry Aspartic Acid - metabolism Biological Transport Protein Conformation Proteolipids - chemistry Pyrococcus horikoshii - chemistry Sodium - chemistry Sodium - metabolism |
| title | Na(+):aspartate coupling stoichiometry in the glutamate transporter homologue Glt(Ph) |
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