Effective solubilization and single-step purification of Bacillus licheniformis α-amylase from insoluble aggregates

A high level expression of thermostable α-amylase gene from Bacillus licheniformis in Escherichia coli was obtained. The recombinant enzyme was mainly produced in the form of insoluble aggregates. The enzyme was solubilized without using denaturing agents and purified to homogeneity in a single step...

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Veröffentlicht in:	Folia microbiologica 2010-03, Vol.55 (2), p.133-136
Hauptverfasser:	Rashid, N., Ahmed, N., Saleem Haider, M., Haque, I.
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Amylases - chemistry alpha-Amylases - isolation & purification Applied Microbiology Bacillus - chemistry Bacillus - enzymology Bacterial Proteins - chemistry Bacterial Proteins - isolation & purification Biomedical and Life Sciences Chromatography, Ion Exchange - methods Environmental Engineering/Biotechnology Immunology Life Sciences Microbiology Solubility
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