The suitability of gastropods as biomarkers: Induction of ethoxy-, pentoxy- and benzoxyresorufin-O-dealkylase in limnetic gastropods via aroclor 1254
The measurement and induction of mixed function oxyigenases (MFOs) of limnetic gastropods were investigated to estimate their suitability as biomarkers. A determination of MFO activities was performed through the measurement of ethoxyresorufin-O-dealkylase (EROD), pentoxy-(PROD) and benzoxyresorufin...
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| Veröffentlicht in: | Environmental science and pollution research international 1997-01, Vol.4 (4), p.183-188 |
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| creator | Meimberg, H Schrenk, C Steinberg, C Klarenberg, A Kettrup, A |
| description | The measurement and induction of mixed function oxyigenases (MFOs) of limnetic gastropods were investigated to estimate their suitability as biomarkers. A determination of MFO activities was performed through the measurement of ethoxyresorufin-O-dealkylase (EROD), pentoxy-(PROD) and benzoxyresorufin-O-dealkylase-activity (BROD). Optimal measuring conditions of these activities were investigated in preparations of the digestive gland of 4 species of limnetic gastropods. Results indicate that MFO-activity inhibiting substances are accumulating in the microsomal pellet, the fraction commonly used for the measurement of MFO-activities. Therefore, the fraction used for induction studies was the postmitochondrial supernatant (PMS). EROD, PROD, and BROD activity of Planorbis planorbis and Planorbis carinatus were measured after a treatment with Aroclor 1254 for 1-17 days. Maximal induction of EROD and PROD were 6 and 10 times the value in the control group of P. carinatus, respectively induction in P. planorbis was lower. BROD-activity could not been measured in P. planorbis. In P. carinatus, BROD-activity increased to a maximum of 2 times after treatment with Aroclor 1254. The level of induction of EROD-activity is comparable to results described in analog studies with fish. |
| doi_str_mv | 10.1007/BF02986340 |
| format | Article |
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A determination of MFO activities was performed through the measurement of ethoxyresorufin-O-dealkylase (EROD), pentoxy-(PROD) and benzoxyresorufin-O-dealkylase-activity (BROD). Optimal measuring conditions of these activities were investigated in preparations of the digestive gland of 4 species of limnetic gastropods. Results indicate that MFO-activity inhibiting substances are accumulating in the microsomal pellet, the fraction commonly used for the measurement of MFO-activities. Therefore, the fraction used for induction studies was the postmitochondrial supernatant (PMS). EROD, PROD, and BROD activity of Planorbis planorbis and Planorbis carinatus were measured after a treatment with Aroclor 1254 for 1-17 days. Maximal induction of EROD and PROD were 6 and 10 times the value in the control group of P. carinatus, respectively induction in P. planorbis was lower. BROD-activity could not been measured in P. planorbis. 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| ispartof | Environmental science and pollution research international, 1997-01, Vol.4 (4), p.183-188 |
| issn | 0944-1344 1614-7499 |
| language | eng |
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| subjects | Gastropoda Planorbis Planorbis planorbis |
| title | The suitability of gastropods as biomarkers: Induction of ethoxy-, pentoxy- and benzoxyresorufin-O-dealkylase in limnetic gastropods via aroclor 1254 |
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