Microspheres of Mixed Proteins
This paper describes the synthesis of mixed proteinaceous microspheres (MPMs) by the sonochemical method. The current fundamental research follows the research of Suslick and co‐workers who have developed a method by which high‐intensity ultrasound is used to make aqueous suspensions of proteinaceou...
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| Veröffentlicht in: | Chemistry : a European journal 2010-02, Vol.16 (7), p.2108-2114 |
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| creator | Angel (Shimanovich), Ulyana Matas, Devorah Michaeli, Shulamit Cavaco-Paulo, Artur Gedanken, Aharon |
| description | This paper describes the synthesis of mixed proteinaceous microspheres (MPMs) by the sonochemical method. The current fundamental research follows the research of Suslick and co‐workers who have developed a method by which high‐intensity ultrasound is used to make aqueous suspensions of proteinaceous microcapsules filled with water‐insoluble liquids.1 By using high‐intensity ultrasound, we have synthesized microspheres made of a few different proteins. The three proteins used in the current experiments are bovine serum albumin (BSA), green fluorescent protein (GFP), and cyan fluorescent protein–glucose binding protein–yellow fluorescent fused protein (CFP‐GBP‐YFP). The two synthesized microspheres made of mixed proteins are BSA‐GFP and BSA‐(CFP‐GBP‐YFP). This paper presents the characterization of the sonochemically produced microspheres of mixed proteins. It also provides an estimate of the efficiency of the sonochemical process in converting the native proteins to microspheres.
Mix it up! By using high‐intensity ultrasound, mixed protein microspheres (MPMs) were synthesized (see figure). The three proteins used in the current experiments are bovine serum albumin (BSA), green fluorescent protein (GFP), and cyan fluorescent protein–glucose binding protein–yellow fluorescent fused protein (CFP‐GBP‐YFP). The two synthesized microspheres made of mixed proteins are BSA‐GFP and BSA‐(CFP‐GBP‐YFP). |
| doi_str_mv | 10.1002/chem.200902098 |
| format | Article |
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Mix it up! By using high‐intensity ultrasound, mixed protein microspheres (MPMs) were synthesized (see figure). The three proteins used in the current experiments are bovine serum albumin (BSA), green fluorescent protein (GFP), and cyan fluorescent protein–glucose binding protein–yellow fluorescent fused protein (CFP‐GBP‐YFP). The two synthesized microspheres made of mixed proteins are BSA‐GFP and BSA‐(CFP‐GBP‐YFP).</description><identifier>ISSN: 0947-6539</identifier><identifier>EISSN: 1521-3765</identifier><identifier>DOI: 10.1002/chem.200902098</identifier><identifier>PMID: 20066692</identifier><identifier>CODEN: CEUJED</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Animals ; Binding ; Cattle ; Chemistry ; core-shell structures ; Escherichia coli - chemistry ; Escherichia coli Proteins - chemistry ; Fluorescence ; Green Fluorescent Proteins - chemistry ; Green Fluorescent Proteins - metabolism ; Humans ; Liquids ; Micelles ; Microscopy, Fluorescence ; Microspheres ; Molecular Structure ; Particle Size ; Protein Binding ; Proteins ; Proteins - chemistry ; Serum albumin ; Serum Albumin - chemistry ; Serum Albumin - metabolism ; Serum Albumin, Bovine - chemistry ; Serum Albumin, Bovine - metabolism ; sonochemistry ; surface modification ; Surface Properties ; Synthesis ; Ultrasonic imaging ; Ultrasound</subject><ispartof>Chemistry : a European journal, 2010-02, Vol.16 (7), p.2108-2114</ispartof><rights>Copyright © 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5088-7db8faaa3214d9eb70bb88ff54f83aa4fdd223a6aaf64216673878a70712dd863</citedby><cites>FETCH-LOGICAL-c5088-7db8faaa3214d9eb70bb88ff54f83aa4fdd223a6aaf64216673878a70712dd863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fchem.200902098$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fchem.200902098$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20066692$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Angel (Shimanovich), Ulyana</creatorcontrib><creatorcontrib>Matas, Devorah</creatorcontrib><creatorcontrib>Michaeli, Shulamit</creatorcontrib><creatorcontrib>Cavaco-Paulo, Artur</creatorcontrib><creatorcontrib>Gedanken, Aharon</creatorcontrib><title>Microspheres of Mixed Proteins</title><title>Chemistry : a European journal</title><addtitle>Chemistry - A European Journal</addtitle><description>This paper describes the synthesis of mixed proteinaceous microspheres (MPMs) by the sonochemical method. The current fundamental research follows the research of Suslick and co‐workers who have developed a method by which high‐intensity ultrasound is used to make aqueous suspensions of proteinaceous microcapsules filled with water‐insoluble liquids.1 By using high‐intensity ultrasound, we have synthesized microspheres made of a few different proteins. The three proteins used in the current experiments are bovine serum albumin (BSA), green fluorescent protein (GFP), and cyan fluorescent protein–glucose binding protein–yellow fluorescent fused protein (CFP‐GBP‐YFP). The two synthesized microspheres made of mixed proteins are BSA‐GFP and BSA‐(CFP‐GBP‐YFP). This paper presents the characterization of the sonochemically produced microspheres of mixed proteins. It also provides an estimate of the efficiency of the sonochemical process in converting the native proteins to microspheres.
Mix it up! By using high‐intensity ultrasound, mixed protein microspheres (MPMs) were synthesized (see figure). The three proteins used in the current experiments are bovine serum albumin (BSA), green fluorescent protein (GFP), and cyan fluorescent protein–glucose binding protein–yellow fluorescent fused protein (CFP‐GBP‐YFP). The two synthesized microspheres made of mixed proteins are BSA‐GFP and BSA‐(CFP‐GBP‐YFP).</description><subject>Animals</subject><subject>Binding</subject><subject>Cattle</subject><subject>Chemistry</subject><subject>core-shell structures</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli Proteins - chemistry</subject><subject>Fluorescence</subject><subject>Green Fluorescent Proteins - chemistry</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Humans</subject><subject>Liquids</subject><subject>Micelles</subject><subject>Microscopy, Fluorescence</subject><subject>Microspheres</subject><subject>Molecular Structure</subject><subject>Particle Size</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Proteins - chemistry</subject><subject>Serum albumin</subject><subject>Serum Albumin - chemistry</subject><subject>Serum Albumin - metabolism</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Serum Albumin, Bovine - metabolism</subject><subject>sonochemistry</subject><subject>surface modification</subject><subject>Surface Properties</subject><subject>Synthesis</subject><subject>Ultrasonic imaging</subject><subject>Ultrasound</subject><issn>0947-6539</issn><issn>1521-3765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAURi0EoqWwMlaVGGBJ8SN-ZERRaUENMPAYLSex1ZSmKXYj2n-Po5QKMcB0l_Md6R4AzhEcIgjxdTbT5RBDGEEMI3EAuohiFBDO6CHowijkAaMk6oAT5-bQY4yQY9DxA8ZYhLugnxSZrdxqpq12g8oMkmKj88GTrda6WLpTcGTUwumz3e2Bl9vRczwJpo_ju_hmGmQUChHwPBVGKUUwCvNIpxymqRDG0NAIolRo8hxjophShoUYMcaJ4EJxyBHOc8FID1y23pWtPmrt1rIsXKYXC7XUVe0kJ4RSyBny5NWfJBIQhhEnnHr04hc6r2q79H9IxP3_PkLYCIct1XRwVhu5skWp7FYiKJvGsmks9439oL_T1mmp8z3-HdUDUQt8Fgu9_Ucn48ko-SkP2m3h1nqz3yr7LlnzlHx7GEuW3E9j8splQr4Af9iUag</recordid><startdate>20100215</startdate><enddate>20100215</enddate><creator>Angel (Shimanovich), Ulyana</creator><creator>Matas, Devorah</creator><creator>Michaeli, Shulamit</creator><creator>Cavaco-Paulo, Artur</creator><creator>Gedanken, Aharon</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20100215</creationdate><title>Microspheres of Mixed Proteins</title><author>Angel (Shimanovich), Ulyana ; Matas, Devorah ; Michaeli, Shulamit ; Cavaco-Paulo, Artur ; Gedanken, Aharon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5088-7db8faaa3214d9eb70bb88ff54f83aa4fdd223a6aaf64216673878a70712dd863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Binding</topic><topic>Cattle</topic><topic>Chemistry</topic><topic>core-shell structures</topic><topic>Escherichia coli - chemistry</topic><topic>Escherichia coli Proteins - chemistry</topic><topic>Fluorescence</topic><topic>Green Fluorescent Proteins - chemistry</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Humans</topic><topic>Liquids</topic><topic>Micelles</topic><topic>Microscopy, Fluorescence</topic><topic>Microspheres</topic><topic>Molecular Structure</topic><topic>Particle Size</topic><topic>Protein Binding</topic><topic>Proteins</topic><topic>Proteins - chemistry</topic><topic>Serum albumin</topic><topic>Serum Albumin - chemistry</topic><topic>Serum Albumin - metabolism</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Serum Albumin, Bovine - metabolism</topic><topic>sonochemistry</topic><topic>surface modification</topic><topic>Surface Properties</topic><topic>Synthesis</topic><topic>Ultrasonic imaging</topic><topic>Ultrasound</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Angel (Shimanovich), Ulyana</creatorcontrib><creatorcontrib>Matas, Devorah</creatorcontrib><creatorcontrib>Michaeli, Shulamit</creatorcontrib><creatorcontrib>Cavaco-Paulo, Artur</creatorcontrib><creatorcontrib>Gedanken, Aharon</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry : a European journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Angel (Shimanovich), Ulyana</au><au>Matas, Devorah</au><au>Michaeli, Shulamit</au><au>Cavaco-Paulo, Artur</au><au>Gedanken, Aharon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microspheres of Mixed Proteins</atitle><jtitle>Chemistry : a European journal</jtitle><addtitle>Chemistry - A European Journal</addtitle><date>2010-02-15</date><risdate>2010</risdate><volume>16</volume><issue>7</issue><spage>2108</spage><epage>2114</epage><pages>2108-2114</pages><issn>0947-6539</issn><eissn>1521-3765</eissn><coden>CEUJED</coden><abstract>This paper describes the synthesis of mixed proteinaceous microspheres (MPMs) by the sonochemical method. The current fundamental research follows the research of Suslick and co‐workers who have developed a method by which high‐intensity ultrasound is used to make aqueous suspensions of proteinaceous microcapsules filled with water‐insoluble liquids.1 By using high‐intensity ultrasound, we have synthesized microspheres made of a few different proteins. The three proteins used in the current experiments are bovine serum albumin (BSA), green fluorescent protein (GFP), and cyan fluorescent protein–glucose binding protein–yellow fluorescent fused protein (CFP‐GBP‐YFP). The two synthesized microspheres made of mixed proteins are BSA‐GFP and BSA‐(CFP‐GBP‐YFP). This paper presents the characterization of the sonochemically produced microspheres of mixed proteins. It also provides an estimate of the efficiency of the sonochemical process in converting the native proteins to microspheres.
Mix it up! By using high‐intensity ultrasound, mixed protein microspheres (MPMs) were synthesized (see figure). The three proteins used in the current experiments are bovine serum albumin (BSA), green fluorescent protein (GFP), and cyan fluorescent protein–glucose binding protein–yellow fluorescent fused protein (CFP‐GBP‐YFP). The two synthesized microspheres made of mixed proteins are BSA‐GFP and BSA‐(CFP‐GBP‐YFP).</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>20066692</pmid><doi>10.1002/chem.200902098</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Binding Cattle Chemistry core-shell structures Escherichia coli - chemistry Escherichia coli Proteins - chemistry Fluorescence Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - metabolism Humans Liquids Micelles Microscopy, Fluorescence Microspheres Molecular Structure Particle Size Protein Binding Proteins Proteins - chemistry Serum albumin Serum Albumin - chemistry Serum Albumin - metabolism Serum Albumin, Bovine - chemistry Serum Albumin, Bovine - metabolism sonochemistry surface modification Surface Properties Synthesis Ultrasonic imaging Ultrasound |
| title | Microspheres of Mixed Proteins |
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