Production of recombinant human antithrombin III on 20-L bioreactor scale: Correlation of supernatant neuraminidase activity, desialylation, and decrease of biological activity of recombinant glycoprotein

Chinese hamster ovary (CHO) cells producing the recombinant glycoprotein human antithrombin III (rhAT III) were batch cultivated in a 20‐L bioreactor for 13 days. Neuraminidase activity in cell‐free supernatant was monitored during cultivation and free sialic acid was determined by HPLC. Neu5Acα(2→3...

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Veröffentlicht in:	Biotechnology and bioengineering 1997-11, Vol.56 (4), p.441-448
Hauptverfasser:	Munzert, Eberhard, Heidemann, Rüdiger, Büntemeyer, Heino, Lehmann, Jürgen, Müthing, Johannes
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cell culture Batch cell culture Bioassay Biological and medical sciences Bioreactors Biotechnology Catalyst activity Chinese hamster ovary (CHO) cells Enzyme kinetics Fundamental and applied biological sciences. Psychology Genetic engineering glycoprotein Growth kinetics Health. Pharmaceutical industry High performance liquid chromatography Industrial applications and implications. Economical aspects neuraminidase activity Other active biomolecules Production of active biomolecules recombinant human antithrombin III (rhAT III)
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