An inducible expression system for the production of human lactoferrin in Aspergillus nidulans
The production and secretion of human lactoferrin (hLF) in Aspergillus nidulans is described. The hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase ( alcA) promoter. Recombinant hLF (rehLF) is produced at levels up to 5 μg/ml. Approximately 30% of the re-...
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| Veröffentlicht in: | Gene 1992-12, Vol.122 (1), p.219-223 |
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| creator | Ward, Pauline P. May, Gregory S. Headon, Denis R. Conneely, Orla M. |
| description | The production and secretion of human lactoferrin (hLF) in
Aspergillus nidulans is described. The
hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase (
alcA) promoter. Recombinant hLF (rehLF) is produced at levels up to 5 μg/ml. Approximately 30% of the re-hLF produced in this system is secreted into the growth medium. The re-hLF is indistinguishable from native hLF with respect to size and immunoreactivity. Furthermore, re-hLF is functional by the criterion of iron-binding capacity. The
A. nidulans expression system offers an inexpensive, convenient method for the controlled production of mg amounts of biologically active mammalian glycoproteins. |
| doi_str_mv | 10.1016/0378-1119(92)90054-S |
| format | Article |
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Aspergillus nidulans is described. The
hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase (
alcA) promoter. Recombinant hLF (rehLF) is produced at levels up to 5 μg/ml. Approximately 30% of the re-hLF produced in this system is secreted into the growth medium. The re-hLF is indistinguishable from native hLF with respect to size and immunoreactivity. Furthermore, re-hLF is functional by the criterion of iron-binding capacity. The
A. nidulans expression system offers an inexpensive, convenient method for the controlled production of mg amounts of biologically active mammalian glycoproteins.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(92)90054-S</identifier><identifier>PMID: 1452033</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>alcA promoter ; alcohol dehydrogenase ; Aspergillus nidulans ; Aspergillus nidulellus ; Biological and medical sciences ; Blotting, Southern ; Blotting, Western ; Cloning, Molecular - methods ; complementary DNA ; DNA ; filamentous fungi ; functional assay ; Fundamental and applied biological sciences. Psychology ; Gene expression ; gene transfer ; Humans ; Iron - metabolism ; iron-binding glycoprotein ; lactoferrin ; Lactoferrin - biosynthesis ; Lactoferrin - genetics ; Lactoferrin - metabolism ; Molecular and cellular biology ; Molecular genetics ; Plasmids ; promoter regions ; protein secretion ; Recombinant DNA ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; secretion ; structural genes ; vectors</subject><ispartof>Gene, 1992-12, Vol.122 (1), p.219-223</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-cada15d3d8ccb1ef9959a55f4021e9daad9b75fccdb24ba1b18baaa01fbaa0203</citedby><cites>FETCH-LOGICAL-c477t-cada15d3d8ccb1ef9959a55f4021e9daad9b75fccdb24ba1b18baaa01fbaa0203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(92)90054-S$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4432380$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1452033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ward, Pauline P.</creatorcontrib><creatorcontrib>May, Gregory S.</creatorcontrib><creatorcontrib>Headon, Denis R.</creatorcontrib><creatorcontrib>Conneely, Orla M.</creatorcontrib><title>An inducible expression system for the production of human lactoferrin in Aspergillus nidulans</title><title>Gene</title><addtitle>Gene</addtitle><description>The production and secretion of human lactoferrin (hLF) in
Aspergillus nidulans is described. The
hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase (
alcA) promoter. Recombinant hLF (rehLF) is produced at levels up to 5 μg/ml. Approximately 30% of the re-hLF produced in this system is secreted into the growth medium. The re-hLF is indistinguishable from native hLF with respect to size and immunoreactivity. Furthermore, re-hLF is functional by the criterion of iron-binding capacity. The
A. nidulans expression system offers an inexpensive, convenient method for the controlled production of mg amounts of biologically active mammalian glycoproteins.</description><subject>alcA promoter</subject><subject>alcohol dehydrogenase</subject><subject>Aspergillus nidulans</subject><subject>Aspergillus nidulellus</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Blotting, Western</subject><subject>Cloning, Molecular - methods</subject><subject>complementary DNA</subject><subject>DNA</subject><subject>filamentous fungi</subject><subject>functional assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>gene transfer</subject><subject>Humans</subject><subject>Iron - metabolism</subject><subject>iron-binding glycoprotein</subject><subject>lactoferrin</subject><subject>Lactoferrin - biosynthesis</subject><subject>Lactoferrin - genetics</subject><subject>Lactoferrin - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Plasmids</subject><subject>promoter regions</subject><subject>protein secretion</subject><subject>Recombinant DNA</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>secretion</subject><subject>structural genes</subject><subject>vectors</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEuP1DAQhC0EWoaFfwDCB4TgELBjO44vSKMVL2klDsNesTp2e9coj8FOEPvvcchouYEvfaivy9VFyFPO3nDGm7dM6LbinJtXpn5tGFOyOtwjO95qUzEm2vtkd4c8JI9y_s7KU6o-I2dcqpoJsSPf9iONo19c7Hqk-OuYMOc4jTTf5hkHGqZE5xukxzQVaF6VKdCbZYCR9uDmKWBKcfWg-3zEdB37fsl0jH7pYcyPyYMAfcYnp3lOrj68_3rxqbr88vHzxf6yclLruXLggSsvfOtcxzEYowwoFSSrORoP4E2nVXDOd7XsgHe87QCA8VAGK5eck5ebb8n5Y8E82yFmh33JgNOSrRZCCW3kf0HeaFbLZnWUG-jSlHPCYI8pDpBuLWd27d-u5dq1XGtq-6d_eyhrz07-Szeg_7u0FV70FycdsoM-JBhdzHeYlKIW7fr78w0LMFm4TgW5OtSMC8Z1Sdg0hXi3EVhq_Rkx2ewijg59TOhm66f476S_AbSNrQk</recordid><startdate>19921201</startdate><enddate>19921201</enddate><creator>Ward, Pauline P.</creator><creator>May, Gregory S.</creator><creator>Headon, Denis R.</creator><creator>Conneely, Orla M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19921201</creationdate><title>An inducible expression system for the production of human lactoferrin in Aspergillus nidulans</title><author>Ward, Pauline P. ; May, Gregory S. ; Headon, Denis R. ; Conneely, Orla M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-cada15d3d8ccb1ef9959a55f4021e9daad9b75fccdb24ba1b18baaa01fbaa0203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>alcA promoter</topic><topic>alcohol dehydrogenase</topic><topic>Aspergillus nidulans</topic><topic>Aspergillus nidulellus</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Blotting, Western</topic><topic>Cloning, Molecular - methods</topic><topic>complementary DNA</topic><topic>DNA</topic><topic>filamentous fungi</topic><topic>functional assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>gene transfer</topic><topic>Humans</topic><topic>Iron - metabolism</topic><topic>iron-binding glycoprotein</topic><topic>lactoferrin</topic><topic>Lactoferrin - biosynthesis</topic><topic>Lactoferrin - genetics</topic><topic>Lactoferrin - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Plasmids</topic><topic>promoter regions</topic><topic>protein secretion</topic><topic>Recombinant DNA</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>secretion</topic><topic>structural genes</topic><topic>vectors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ward, Pauline P.</creatorcontrib><creatorcontrib>May, Gregory S.</creatorcontrib><creatorcontrib>Headon, Denis R.</creatorcontrib><creatorcontrib>Conneely, Orla M.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ward, Pauline P.</au><au>May, Gregory S.</au><au>Headon, Denis R.</au><au>Conneely, Orla M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An inducible expression system for the production of human lactoferrin in Aspergillus nidulans</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1992-12-01</date><risdate>1992</risdate><volume>122</volume><issue>1</issue><spage>219</spage><epage>223</epage><pages>219-223</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>The production and secretion of human lactoferrin (hLF) in
Aspergillus nidulans is described. The
hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase (
alcA) promoter. Recombinant hLF (rehLF) is produced at levels up to 5 μg/ml. Approximately 30% of the re-hLF produced in this system is secreted into the growth medium. The re-hLF is indistinguishable from native hLF with respect to size and immunoreactivity. Furthermore, re-hLF is functional by the criterion of iron-binding capacity. The
A. nidulans expression system offers an inexpensive, convenient method for the controlled production of mg amounts of biologically active mammalian glycoproteins.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>1452033</pmid><doi>10.1016/0378-1119(92)90054-S</doi><tpages>5</tpages></addata></record> |
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| subjects | alcA promoter alcohol dehydrogenase Aspergillus nidulans Aspergillus nidulellus Biological and medical sciences Blotting, Southern Blotting, Western Cloning, Molecular - methods complementary DNA DNA filamentous fungi functional assay Fundamental and applied biological sciences. Psychology Gene expression gene transfer Humans Iron - metabolism iron-binding glycoprotein lactoferrin Lactoferrin - biosynthesis Lactoferrin - genetics Lactoferrin - metabolism Molecular and cellular biology Molecular genetics Plasmids promoter regions protein secretion Recombinant DNA Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - metabolism secretion structural genes vectors |
| title | An inducible expression system for the production of human lactoferrin in Aspergillus nidulans |
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