Serum-protein effects on the detection of the β-blocker propranolol by ion-transfer voltammetry at a micro-ITIES array

In this work, the effect of the serum protein, bovine serum albumin (BSA), on the detection of propranolol in artificial serum by ion-transfer voltammetry at an array of micro-interfaces between two immiscible electrolyte solutions (μITIES) is presented. Cyclic voltammetry (CV), differential pulse v...

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Veröffentlicht in:Talanta (Oxford) 2010-03, Vol.80 (5), p.1993-1998
Hauptverfasser: Collins, Courtney J., Lyons, Conor, Strutwolf, Jörg, Arrigan, Damien W.M.
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container_end_page 1998
container_issue 5
container_start_page 1993
container_title Talanta (Oxford)
container_volume 80
creator Collins, Courtney J.
Lyons, Conor
Strutwolf, Jörg
Arrigan, Damien W.M.
description In this work, the effect of the serum protein, bovine serum albumin (BSA), on the detection of propranolol in artificial serum by ion-transfer voltammetry at an array of micro-interfaces between two immiscible electrolyte solutions (μITIES) is presented. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and differential pulse stripping voltammetry (DPSV) were examined for the detection of low concentrations of propranolol. Both CV and DPV had an interference effect from BSA, manifested as lower currents in the presence of the protein. DPSV proved to be the most effective technique, enabling the detection of 0.05 μM propranolol in the presence of BSA. The DPSV method employed a preconditioning step as well as a preconcentration step followed by the analytical signal generation step. The latter was based on the back-transfer of the drug across the μITIES. The preconcentration step was crucial to prevention of the adverse effects of BSA on the voltammetric detection. These results demonstrate that serum-protein effects on drug detection at low concentrations can be eliminated by use of DPSV at arrays of μITIES. CVs of propranolol with increasing concentrations of BSA revealed the influence of the drug–protein binding interaction, with decreases in current but no change in transfer potential. Therapeutic concentrations of propranolol were detected, demonstrating the viability of this approach for bioanalytical investigations.
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subjects Adrenergic beta-Antagonists - blood
Adrenergic beta-Antagonists - metabolism
Analytical chemistry
Animals
Bioanalytical
Cattle
Chemistry
Drug monitoring
Electrochemical methods
Electrochemical Techniques - methods
Exact sciences and technology
Immiscible electrolyte solutions
Liquid/liquid interface
Propranolol - blood
Propranolol - metabolism
Protein Binding
Serum Albumin, Bovine - metabolism
Voltammetry
title Serum-protein effects on the detection of the β-blocker propranolol by ion-transfer voltammetry at a micro-ITIES array
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