Serum-protein effects on the detection of the β-blocker propranolol by ion-transfer voltammetry at a micro-ITIES array
In this work, the effect of the serum protein, bovine serum albumin (BSA), on the detection of propranolol in artificial serum by ion-transfer voltammetry at an array of micro-interfaces between two immiscible electrolyte solutions (μITIES) is presented. Cyclic voltammetry (CV), differential pulse v...
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Veröffentlicht in: | Talanta (Oxford) 2010-03, Vol.80 (5), p.1993-1998 |
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creator | Collins, Courtney J. Lyons, Conor Strutwolf, Jörg Arrigan, Damien W.M. |
description | In this work, the effect of the serum protein, bovine serum albumin (BSA), on the detection of propranolol in artificial serum by ion-transfer voltammetry at an array of micro-interfaces between two immiscible electrolyte solutions (μITIES) is presented. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and differential pulse stripping voltammetry (DPSV) were examined for the detection of low concentrations of propranolol. Both CV and DPV had an interference effect from BSA, manifested as lower currents in the presence of the protein. DPSV proved to be the most effective technique, enabling the detection of 0.05
μM propranolol in the presence of BSA. The DPSV method employed a preconditioning step as well as a preconcentration step followed by the analytical signal generation step. The latter was based on the back-transfer of the drug across the μITIES. The preconcentration step was crucial to prevention of the adverse effects of BSA on the voltammetric detection. These results demonstrate that serum-protein effects on drug detection at low concentrations can be eliminated by use of DPSV at arrays of μITIES. CVs of propranolol with increasing concentrations of BSA revealed the influence of the drug–protein binding interaction, with decreases in current but no change in transfer potential. Therapeutic concentrations of propranolol were detected, demonstrating the viability of this approach for bioanalytical investigations. |
doi_str_mv | 10.1016/j.talanta.2009.10.060 |
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μM propranolol in the presence of BSA. The DPSV method employed a preconditioning step as well as a preconcentration step followed by the analytical signal generation step. The latter was based on the back-transfer of the drug across the μITIES. The preconcentration step was crucial to prevention of the adverse effects of BSA on the voltammetric detection. These results demonstrate that serum-protein effects on drug detection at low concentrations can be eliminated by use of DPSV at arrays of μITIES. CVs of propranolol with increasing concentrations of BSA revealed the influence of the drug–protein binding interaction, with decreases in current but no change in transfer potential. Therapeutic concentrations of propranolol were detected, demonstrating the viability of this approach for bioanalytical investigations.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2009.10.060</identifier><identifier>PMID: 20152444</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adrenergic beta-Antagonists - blood ; Adrenergic beta-Antagonists - metabolism ; Analytical chemistry ; Animals ; Bioanalytical ; Cattle ; Chemistry ; Drug monitoring ; Electrochemical methods ; Electrochemical Techniques - methods ; Exact sciences and technology ; Immiscible electrolyte solutions ; Liquid/liquid interface ; Propranolol - blood ; Propranolol - metabolism ; Protein Binding ; Serum Albumin, Bovine - metabolism ; Voltammetry</subject><ispartof>Talanta (Oxford), 2010-03, Vol.80 (5), p.1993-1998</ispartof><rights>2009 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright (c) 2009 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-881fbbb0e7fd12a42348a160c359d82d16d13141629dbddcbdb7607b85bd53093</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2009.10.060$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22551056$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20152444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Collins, Courtney J.</creatorcontrib><creatorcontrib>Lyons, Conor</creatorcontrib><creatorcontrib>Strutwolf, Jörg</creatorcontrib><creatorcontrib>Arrigan, Damien W.M.</creatorcontrib><title>Serum-protein effects on the detection of the β-blocker propranolol by ion-transfer voltammetry at a micro-ITIES array</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>In this work, the effect of the serum protein, bovine serum albumin (BSA), on the detection of propranolol in artificial serum by ion-transfer voltammetry at an array of micro-interfaces between two immiscible electrolyte solutions (μITIES) is presented. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and differential pulse stripping voltammetry (DPSV) were examined for the detection of low concentrations of propranolol. Both CV and DPV had an interference effect from BSA, manifested as lower currents in the presence of the protein. DPSV proved to be the most effective technique, enabling the detection of 0.05
μM propranolol in the presence of BSA. The DPSV method employed a preconditioning step as well as a preconcentration step followed by the analytical signal generation step. The latter was based on the back-transfer of the drug across the μITIES. The preconcentration step was crucial to prevention of the adverse effects of BSA on the voltammetric detection. These results demonstrate that serum-protein effects on drug detection at low concentrations can be eliminated by use of DPSV at arrays of μITIES. CVs of propranolol with increasing concentrations of BSA revealed the influence of the drug–protein binding interaction, with decreases in current but no change in transfer potential. Therapeutic concentrations of propranolol were detected, demonstrating the viability of this approach for bioanalytical investigations.</description><subject>Adrenergic beta-Antagonists - blood</subject><subject>Adrenergic beta-Antagonists - metabolism</subject><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Bioanalytical</subject><subject>Cattle</subject><subject>Chemistry</subject><subject>Drug monitoring</subject><subject>Electrochemical methods</subject><subject>Electrochemical Techniques - methods</subject><subject>Exact sciences and technology</subject><subject>Immiscible electrolyte solutions</subject><subject>Liquid/liquid interface</subject><subject>Propranolol - blood</subject><subject>Propranolol - metabolism</subject><subject>Protein Binding</subject><subject>Serum Albumin, Bovine - metabolism</subject><subject>Voltammetry</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuOEzEQhi0EYsLAEUDeIFYdyna7HyuERgNEGonFDGvLj7Jw6G4H25lRrsVBOBMOCbBkZVX5K9evz4S8ZLBmwLq323XRk16KXnOAsfbW0MEjsmJDLxohe_GYrADE2IyshQvyLOctAHAB4im54MAkb9t2RR5uMe3nZpdiwbBQ9B5tyTQutHxF6rDUMtQq-t-Nnz8aM0X7DROtI7uklzjFiZoDrVBTap19vbuPU9HzjCUdqC5U0znYFJvN3eb6luqU9OE5eeL1lPHF-bwkXz5c3119am4-f9xcvb9prBjb0gwD88YYwN47xnXLRTto1oEVcnQDd6xzTLCWdXx0xjlrnOk76M0gjZMCRnFJ3pzerXG_7zEXNYdscarqMO6z6oWQfOxHVkl5ImvSnBN6tUth1umgGKijcrVVZ-XqqPzYrsrr3Kvzhr2Z0f2d-uO4Aq_PgM5WT75KsiH_47iUDGRXuXcnDquP-4BJZRtwsehCqr-gXAz_ifIL216jpw</recordid><startdate>20100315</startdate><enddate>20100315</enddate><creator>Collins, Courtney J.</creator><creator>Lyons, Conor</creator><creator>Strutwolf, Jörg</creator><creator>Arrigan, Damien W.M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100315</creationdate><title>Serum-protein effects on the detection of the β-blocker propranolol by ion-transfer voltammetry at a micro-ITIES array</title><author>Collins, Courtney J. ; Lyons, Conor ; Strutwolf, Jörg ; Arrigan, Damien W.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-881fbbb0e7fd12a42348a160c359d82d16d13141629dbddcbdb7607b85bd53093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adrenergic beta-Antagonists - blood</topic><topic>Adrenergic beta-Antagonists - metabolism</topic><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Bioanalytical</topic><topic>Cattle</topic><topic>Chemistry</topic><topic>Drug monitoring</topic><topic>Electrochemical methods</topic><topic>Electrochemical Techniques - methods</topic><topic>Exact sciences and technology</topic><topic>Immiscible electrolyte solutions</topic><topic>Liquid/liquid interface</topic><topic>Propranolol - blood</topic><topic>Propranolol - metabolism</topic><topic>Protein Binding</topic><topic>Serum Albumin, Bovine - metabolism</topic><topic>Voltammetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Collins, Courtney J.</creatorcontrib><creatorcontrib>Lyons, Conor</creatorcontrib><creatorcontrib>Strutwolf, Jörg</creatorcontrib><creatorcontrib>Arrigan, Damien W.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Collins, Courtney J.</au><au>Lyons, Conor</au><au>Strutwolf, Jörg</au><au>Arrigan, Damien W.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum-protein effects on the detection of the β-blocker propranolol by ion-transfer voltammetry at a micro-ITIES array</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2010-03-15</date><risdate>2010</risdate><volume>80</volume><issue>5</issue><spage>1993</spage><epage>1998</epage><pages>1993-1998</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>In this work, the effect of the serum protein, bovine serum albumin (BSA), on the detection of propranolol in artificial serum by ion-transfer voltammetry at an array of micro-interfaces between two immiscible electrolyte solutions (μITIES) is presented. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and differential pulse stripping voltammetry (DPSV) were examined for the detection of low concentrations of propranolol. Both CV and DPV had an interference effect from BSA, manifested as lower currents in the presence of the protein. DPSV proved to be the most effective technique, enabling the detection of 0.05
μM propranolol in the presence of BSA. The DPSV method employed a preconditioning step as well as a preconcentration step followed by the analytical signal generation step. The latter was based on the back-transfer of the drug across the μITIES. The preconcentration step was crucial to prevention of the adverse effects of BSA on the voltammetric detection. These results demonstrate that serum-protein effects on drug detection at low concentrations can be eliminated by use of DPSV at arrays of μITIES. CVs of propranolol with increasing concentrations of BSA revealed the influence of the drug–protein binding interaction, with decreases in current but no change in transfer potential. Therapeutic concentrations of propranolol were detected, demonstrating the viability of this approach for bioanalytical investigations.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20152444</pmid><doi>10.1016/j.talanta.2009.10.060</doi><tpages>6</tpages></addata></record> |
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subjects | Adrenergic beta-Antagonists - blood Adrenergic beta-Antagonists - metabolism Analytical chemistry Animals Bioanalytical Cattle Chemistry Drug monitoring Electrochemical methods Electrochemical Techniques - methods Exact sciences and technology Immiscible electrolyte solutions Liquid/liquid interface Propranolol - blood Propranolol - metabolism Protein Binding Serum Albumin, Bovine - metabolism Voltammetry |
title | Serum-protein effects on the detection of the β-blocker propranolol by ion-transfer voltammetry at a micro-ITIES array |
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