Uptake of 11C-choline in mouse atherosclerotic plaques
The purpose of this study was to explore the feasibility of (11)C-choline in the assessment of the degree of inflammation in atherosclerotic plaques. Uptake of (11)C-choline was studied ex vivo in tissue samples and aortic sections excised from 6 atherosclerotic mice deficient for both low-density l...
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| Veröffentlicht in: | The Journal of nuclear medicine (1978) 2010-05, Vol.51 (5), p.798-802 |
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| container_title | The Journal of nuclear medicine (1978) |
| container_volume | 51 |
| creator | Laitinen, Iina E K Luoto, Pauliina Någren, Kjell Marjamäki, Päivi M Silvola, Johanna M U Hellberg, Sanna Laine, V Jukka O Ylä-Herttuala, Seppo Knuuti, Juhani Roivainen, Anne |
| description | The purpose of this study was to explore the feasibility of (11)C-choline in the assessment of the degree of inflammation in atherosclerotic plaques.
Uptake of (11)C-choline was studied ex vivo in tissue samples and aortic sections excised from 6 atherosclerotic mice deficient for both low-density lipoprotein receptor and apolipoprotein B48 (LDLR(-/-)ApoB(100/100)) and 5 control mice. The autoradiographs were compared with the immunohistology of the arterial sites.
The uptake of (11)C-choline (percentage of the injected activity per gram of tissue) in the atherosclerotic aortas of the LDLR(-/-)ApoB(100/100) mice was significantly higher (1.9-fold, P = 0.0016) than that in the aortas of the control mice. The autoradiography analysis showed significantly higher uptake of (11)C-choline in the plaques than in healthy vessel wall (mean ratio, 2.3 +/- 0.6; P = 0.014), prominently in inflamed plaques, compared with noninflamed plaque areas.
We observed a high (11)C-choline uptake in the aortic plaques of atherosclerotic mice. Our data suggest that macrophages may be responsible for the uptake of (11)C-choline in the plaques. |
| doi_str_mv | 10.2967/jnumed.109.071704 |
| format | Article |
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Uptake of (11)C-choline was studied ex vivo in tissue samples and aortic sections excised from 6 atherosclerotic mice deficient for both low-density lipoprotein receptor and apolipoprotein B48 (LDLR(-/-)ApoB(100/100)) and 5 control mice. The autoradiographs were compared with the immunohistology of the arterial sites.
The uptake of (11)C-choline (percentage of the injected activity per gram of tissue) in the atherosclerotic aortas of the LDLR(-/-)ApoB(100/100) mice was significantly higher (1.9-fold, P = 0.0016) than that in the aortas of the control mice. The autoradiography analysis showed significantly higher uptake of (11)C-choline in the plaques than in healthy vessel wall (mean ratio, 2.3 +/- 0.6; P = 0.014), prominently in inflamed plaques, compared with noninflamed plaque areas.
We observed a high (11)C-choline uptake in the aortic plaques of atherosclerotic mice. Our data suggest that macrophages may be responsible for the uptake of (11)C-choline in the plaques.</description><identifier>EISSN: 1535-5667</identifier><identifier>DOI: 10.2967/jnumed.109.071704</identifier><identifier>PMID: 20395326</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Apolipoproteins B - genetics ; Atherosclerosis - diagnostic imaging ; Atherosclerosis - genetics ; Atherosclerosis - metabolism ; Choline - pharmacokinetics ; Cryopreservation ; Immunohistochemistry ; Inflammation - diagnostic imaging ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Radionuclide Imaging ; Radiopharmaceuticals - pharmacokinetics ; Receptors, LDL - genetics ; Tissue Distribution</subject><ispartof>The Journal of nuclear medicine (1978), 2010-05, Vol.51 (5), p.798-802</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20395326$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laitinen, Iina E K</creatorcontrib><creatorcontrib>Luoto, Pauliina</creatorcontrib><creatorcontrib>Någren, Kjell</creatorcontrib><creatorcontrib>Marjamäki, Päivi M</creatorcontrib><creatorcontrib>Silvola, Johanna M U</creatorcontrib><creatorcontrib>Hellberg, Sanna</creatorcontrib><creatorcontrib>Laine, V Jukka O</creatorcontrib><creatorcontrib>Ylä-Herttuala, Seppo</creatorcontrib><creatorcontrib>Knuuti, Juhani</creatorcontrib><creatorcontrib>Roivainen, Anne</creatorcontrib><title>Uptake of 11C-choline in mouse atherosclerotic plaques</title><title>The Journal of nuclear medicine (1978)</title><addtitle>J Nucl Med</addtitle><description>The purpose of this study was to explore the feasibility of (11)C-choline in the assessment of the degree of inflammation in atherosclerotic plaques.
Uptake of (11)C-choline was studied ex vivo in tissue samples and aortic sections excised from 6 atherosclerotic mice deficient for both low-density lipoprotein receptor and apolipoprotein B48 (LDLR(-/-)ApoB(100/100)) and 5 control mice. The autoradiographs were compared with the immunohistology of the arterial sites.
The uptake of (11)C-choline (percentage of the injected activity per gram of tissue) in the atherosclerotic aortas of the LDLR(-/-)ApoB(100/100) mice was significantly higher (1.9-fold, P = 0.0016) than that in the aortas of the control mice. The autoradiography analysis showed significantly higher uptake of (11)C-choline in the plaques than in healthy vessel wall (mean ratio, 2.3 +/- 0.6; P = 0.014), prominently in inflamed plaques, compared with noninflamed plaque areas.
We observed a high (11)C-choline uptake in the aortic plaques of atherosclerotic mice. Our data suggest that macrophages may be responsible for the uptake of (11)C-choline in the plaques.</description><subject>Animals</subject><subject>Apolipoproteins B - genetics</subject><subject>Atherosclerosis - diagnostic imaging</subject><subject>Atherosclerosis - genetics</subject><subject>Atherosclerosis - metabolism</subject><subject>Choline - pharmacokinetics</subject><subject>Cryopreservation</subject><subject>Immunohistochemistry</subject><subject>Inflammation - diagnostic imaging</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Radionuclide Imaging</subject><subject>Radiopharmaceuticals - pharmacokinetics</subject><subject>Receptors, LDL - genetics</subject><subject>Tissue Distribution</subject><issn>1535-5667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1UDtPwzAYtJAQLYUfwIK8MSX4-dkeUQQFqRILnSPbsdWEvIiTgX9PEGW5W073QuiOkpwZUI9Nv3ShyikxOVFUEXGBtlRymUkAtUHXKTWEENBaX6ENI9xIzmCL4DjO9jPgIWJKi8yfhrbuA6573A1LCtjOpzANybcrzrXHY2u_lpBu0GW0bQq3Z96h48vzR_GaHd73b8XTIWs4NXMmJBhlgQqndLTUe-lBaeKh8pFD5VgU1BuhwVgpIzEhMkGk0-CclVUl-Q49_PmO0_CbO5ddnXxoW9uHtV-pOJcMKDOr8v6sXNx6RDlOdWen7_J_Kv8B7vlUQA</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Laitinen, Iina E K</creator><creator>Luoto, Pauliina</creator><creator>Någren, Kjell</creator><creator>Marjamäki, Päivi M</creator><creator>Silvola, Johanna M U</creator><creator>Hellberg, Sanna</creator><creator>Laine, V Jukka O</creator><creator>Ylä-Herttuala, Seppo</creator><creator>Knuuti, Juhani</creator><creator>Roivainen, Anne</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20100501</creationdate><title>Uptake of 11C-choline in mouse atherosclerotic plaques</title><author>Laitinen, Iina E K ; Luoto, Pauliina ; Någren, Kjell ; Marjamäki, Päivi M ; Silvola, Johanna M U ; Hellberg, Sanna ; Laine, V Jukka O ; Ylä-Herttuala, Seppo ; Knuuti, Juhani ; Roivainen, Anne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j319t-45697a614b78fa1cc5c6780c6dcf36db2f41c94869a55f09ef2405b86bba5dd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Apolipoproteins B - genetics</topic><topic>Atherosclerosis - diagnostic imaging</topic><topic>Atherosclerosis - genetics</topic><topic>Atherosclerosis - metabolism</topic><topic>Choline - pharmacokinetics</topic><topic>Cryopreservation</topic><topic>Immunohistochemistry</topic><topic>Inflammation - diagnostic imaging</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Radionuclide Imaging</topic><topic>Radiopharmaceuticals - pharmacokinetics</topic><topic>Receptors, LDL - genetics</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laitinen, Iina E K</creatorcontrib><creatorcontrib>Luoto, Pauliina</creatorcontrib><creatorcontrib>Någren, Kjell</creatorcontrib><creatorcontrib>Marjamäki, Päivi M</creatorcontrib><creatorcontrib>Silvola, Johanna M U</creatorcontrib><creatorcontrib>Hellberg, Sanna</creatorcontrib><creatorcontrib>Laine, V Jukka O</creatorcontrib><creatorcontrib>Ylä-Herttuala, Seppo</creatorcontrib><creatorcontrib>Knuuti, Juhani</creatorcontrib><creatorcontrib>Roivainen, Anne</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nuclear medicine (1978)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laitinen, Iina E K</au><au>Luoto, Pauliina</au><au>Någren, Kjell</au><au>Marjamäki, Päivi M</au><au>Silvola, Johanna M U</au><au>Hellberg, Sanna</au><au>Laine, V Jukka O</au><au>Ylä-Herttuala, Seppo</au><au>Knuuti, Juhani</au><au>Roivainen, Anne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Uptake of 11C-choline in mouse atherosclerotic plaques</atitle><jtitle>The Journal of nuclear medicine (1978)</jtitle><addtitle>J Nucl Med</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>51</volume><issue>5</issue><spage>798</spage><epage>802</epage><pages>798-802</pages><eissn>1535-5667</eissn><abstract>The purpose of this study was to explore the feasibility of (11)C-choline in the assessment of the degree of inflammation in atherosclerotic plaques.
Uptake of (11)C-choline was studied ex vivo in tissue samples and aortic sections excised from 6 atherosclerotic mice deficient for both low-density lipoprotein receptor and apolipoprotein B48 (LDLR(-/-)ApoB(100/100)) and 5 control mice. The autoradiographs were compared with the immunohistology of the arterial sites.
The uptake of (11)C-choline (percentage of the injected activity per gram of tissue) in the atherosclerotic aortas of the LDLR(-/-)ApoB(100/100) mice was significantly higher (1.9-fold, P = 0.0016) than that in the aortas of the control mice. The autoradiography analysis showed significantly higher uptake of (11)C-choline in the plaques than in healthy vessel wall (mean ratio, 2.3 +/- 0.6; P = 0.014), prominently in inflamed plaques, compared with noninflamed plaque areas.
We observed a high (11)C-choline uptake in the aortic plaques of atherosclerotic mice. Our data suggest that macrophages may be responsible for the uptake of (11)C-choline in the plaques.</abstract><cop>United States</cop><pmid>20395326</pmid><doi>10.2967/jnumed.109.071704</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of nuclear medicine (1978), 2010-05, Vol.51 (5), p.798-802 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Apolipoproteins B - genetics Atherosclerosis - diagnostic imaging Atherosclerosis - genetics Atherosclerosis - metabolism Choline - pharmacokinetics Cryopreservation Immunohistochemistry Inflammation - diagnostic imaging Male Mice Mice, Inbred C57BL Mice, Knockout Radionuclide Imaging Radiopharmaceuticals - pharmacokinetics Receptors, LDL - genetics Tissue Distribution |
| title | Uptake of 11C-choline in mouse atherosclerotic plaques |
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