Inhibitory effect of activated protein C on platelet aggregation induced by the prothrombin-converting reaction
The present study was undertaken to elucidate the effect on platelet aggregation of the prothrombin-converting reaction on platelets with or without activated protein C (APC). A reaction mixture of washed platelets from human individuals, Factor Xa and prothrombin markedly induced platelet aggregati...
Gespeichert in:
| Veröffentlicht in: | Thrombosis research 1992-09, Vol.67 (6), p.705-710 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 710 |
|---|---|
| container_issue | 6 |
| container_start_page | 705 |
| container_title | Thrombosis research |
| container_volume | 67 |
| creator | Komoriya, Keiji Inoue, Kyoko Takahashi, Katsushi Suzuki, Koji |
| description | The present study was undertaken to elucidate the effect on platelet aggregation of the prothrombin-converting reaction on platelets with or without activated protein C (APC). A reaction mixture of washed platelets from human individuals, Factor Xa and prothrombin markedly induced platelet aggregation; maximum aggregation rates, 31.3–92.5%, and times to reach to maximum aggregation, 11.6 to 20.1 min. This aggregation was inhibited by the addition of APC with 50% inhibition concentration (IC
50) value of 14.4 U/ml. APC also inhibited thrombin generation in the reaction mixture in a dose-dependent manner with IC50 value of 0.96 U/ml. However, APC did not inhibit the thrombin (0.1 CU/ml)-induced platelet aggregation at concentrations of up to 30 U/ml. These findings suggest that APC has no direct inhibitory effect on platelet aggregation and that APC inhibits platelet aggregation through inhibition of thrombin generation. |
| doi_str_mv | 10.1016/0049-3848(92)90074-K |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73352122</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>004938489290074K</els_id><sourcerecordid>73352122</sourcerecordid><originalsourceid>FETCH-LOGICAL-c452t-65fe592461dee078efe2a6bce0752b46f687fc6f278059c01c82cbb4d24a369e3</originalsourceid><addsrcrecordid>eNp9kcFuGyEQhlHUKHHTvEEicaiq9rAJsLAsl0iV1TZRIuWSnhHLDjbRGlzAlvz2xbGV3noCzXz_aPQNQleU3FBCu1tCuGranvdfFfumCJG8eTxBM9pL1TAu2Qc0e0fO0cecXwmhkipxhs4o50S0YobiQ1j6wZeYdhicA1twdNjY4remwIjXKRbwAc9xDHg91doEBZvFIsHCFF-LPowbW8lhh8sS3gLLFFeDD42NYQup-LDACfYzY_iETp2ZMlwe3wv0--ePl_l98_T862H-_amxXLDSdMKBUIx3dAQgsgcHzHSDrX_BBt65rpfOdo7JnghlCbU9s8PAR8ZN2yloL9CXw9y6z58N5KJXPluYJhMgbrKWbSsYZayC_ADaFHNO4PQ6-ZVJO02J3nvWe4l6L1Erpt8868cauz7O3wwrGP-FDmJr__Oxb7I1k0smWJ_fMc57JiWp2N0Bg-pi6yHpbD2E6tOnegs9Rv__Pf4C8jWbcw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73352122</pqid></control><display><type>article</type><title>Inhibitory effect of activated protein C on platelet aggregation induced by the prothrombin-converting reaction</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Komoriya, Keiji ; Inoue, Kyoko ; Takahashi, Katsushi ; Suzuki, Koji</creator><creatorcontrib>Komoriya, Keiji ; Inoue, Kyoko ; Takahashi, Katsushi ; Suzuki, Koji</creatorcontrib><description>The present study was undertaken to elucidate the effect on platelet aggregation of the prothrombin-converting reaction on platelets with or without activated protein C (APC). A reaction mixture of washed platelets from human individuals, Factor Xa and prothrombin markedly induced platelet aggregation; maximum aggregation rates, 31.3–92.5%, and times to reach to maximum aggregation, 11.6 to 20.1 min. This aggregation was inhibited by the addition of APC with 50% inhibition concentration (IC
50) value of 14.4 U/ml. APC also inhibited thrombin generation in the reaction mixture in a dose-dependent manner with IC50 value of 0.96 U/ml. However, APC did not inhibit the thrombin (0.1 CU/ml)-induced platelet aggregation at concentrations of up to 30 U/ml. These findings suggest that APC has no direct inhibitory effect on platelet aggregation and that APC inhibits platelet aggregation through inhibition of thrombin generation.</description><identifier>ISSN: 0049-3848</identifier><identifier>EISSN: 1879-2472</identifier><identifier>DOI: 10.1016/0049-3848(92)90074-K</identifier><identifier>PMID: 1440535</identifier><identifier>CODEN: THBRAA</identifier><language>eng</language><publisher>New York, NY: Elsevier Ltd</publisher><subject>activated protein C ; Biological and medical sciences ; Blood coagulation. Blood cells ; Blood Platelets - drug effects ; Blood Platelets - metabolism ; Coumarins ; Factor Xa - metabolism ; Factor Xa - pharmacology ; Fluorescent Dyes ; Fundamental and applied biological sciences. Psychology ; Humans ; In Vitro Techniques ; Molecular and cellular biology ; Oligopeptides ; Platelet ; platelet aggregation ; Platelet Aggregation - drug effects ; Platelet Aggregation - physiology ; Platelet Aggregation Inhibitors - pharmacology ; Protein C - metabolism ; Protein C - pharmacology ; Prothrombin - metabolism ; Prothrombin - pharmacology ; thrombin ; Thrombin - biosynthesis ; Thrombin - pharmacology</subject><ispartof>Thrombosis research, 1992-09, Vol.67 (6), p.705-710</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-65fe592461dee078efe2a6bce0752b46f687fc6f278059c01c82cbb4d24a369e3</citedby><cites>FETCH-LOGICAL-c452t-65fe592461dee078efe2a6bce0752b46f687fc6f278059c01c82cbb4d24a369e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/004938489290074K$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4482770$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1440535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Komoriya, Keiji</creatorcontrib><creatorcontrib>Inoue, Kyoko</creatorcontrib><creatorcontrib>Takahashi, Katsushi</creatorcontrib><creatorcontrib>Suzuki, Koji</creatorcontrib><title>Inhibitory effect of activated protein C on platelet aggregation induced by the prothrombin-converting reaction</title><title>Thrombosis research</title><addtitle>Thromb Res</addtitle><description>The present study was undertaken to elucidate the effect on platelet aggregation of the prothrombin-converting reaction on platelets with or without activated protein C (APC). A reaction mixture of washed platelets from human individuals, Factor Xa and prothrombin markedly induced platelet aggregation; maximum aggregation rates, 31.3–92.5%, and times to reach to maximum aggregation, 11.6 to 20.1 min. This aggregation was inhibited by the addition of APC with 50% inhibition concentration (IC
50) value of 14.4 U/ml. APC also inhibited thrombin generation in the reaction mixture in a dose-dependent manner with IC50 value of 0.96 U/ml. However, APC did not inhibit the thrombin (0.1 CU/ml)-induced platelet aggregation at concentrations of up to 30 U/ml. These findings suggest that APC has no direct inhibitory effect on platelet aggregation and that APC inhibits platelet aggregation through inhibition of thrombin generation.</description><subject>activated protein C</subject><subject>Biological and medical sciences</subject><subject>Blood coagulation. Blood cells</subject><subject>Blood Platelets - drug effects</subject><subject>Blood Platelets - metabolism</subject><subject>Coumarins</subject><subject>Factor Xa - metabolism</subject><subject>Factor Xa - pharmacology</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Molecular and cellular biology</subject><subject>Oligopeptides</subject><subject>Platelet</subject><subject>platelet aggregation</subject><subject>Platelet Aggregation - drug effects</subject><subject>Platelet Aggregation - physiology</subject><subject>Platelet Aggregation Inhibitors - pharmacology</subject><subject>Protein C - metabolism</subject><subject>Protein C - pharmacology</subject><subject>Prothrombin - metabolism</subject><subject>Prothrombin - pharmacology</subject><subject>thrombin</subject><subject>Thrombin - biosynthesis</subject><subject>Thrombin - pharmacology</subject><issn>0049-3848</issn><issn>1879-2472</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFuGyEQhlHUKHHTvEEicaiq9rAJsLAsl0iV1TZRIuWSnhHLDjbRGlzAlvz2xbGV3noCzXz_aPQNQleU3FBCu1tCuGranvdfFfumCJG8eTxBM9pL1TAu2Qc0e0fO0cecXwmhkipxhs4o50S0YobiQ1j6wZeYdhicA1twdNjY4remwIjXKRbwAc9xDHg91doEBZvFIsHCFF-LPowbW8lhh8sS3gLLFFeDD42NYQup-LDACfYzY_iETp2ZMlwe3wv0--ePl_l98_T862H-_amxXLDSdMKBUIx3dAQgsgcHzHSDrX_BBt65rpfOdo7JnghlCbU9s8PAR8ZN2yloL9CXw9y6z58N5KJXPluYJhMgbrKWbSsYZayC_ADaFHNO4PQ6-ZVJO02J3nvWe4l6L1Erpt8868cauz7O3wwrGP-FDmJr__Oxb7I1k0smWJ_fMc57JiWp2N0Bg-pi6yHpbD2E6tOnegs9Rv__Pf4C8jWbcw</recordid><startdate>19920915</startdate><enddate>19920915</enddate><creator>Komoriya, Keiji</creator><creator>Inoue, Kyoko</creator><creator>Takahashi, Katsushi</creator><creator>Suzuki, Koji</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920915</creationdate><title>Inhibitory effect of activated protein C on platelet aggregation induced by the prothrombin-converting reaction</title><author>Komoriya, Keiji ; Inoue, Kyoko ; Takahashi, Katsushi ; Suzuki, Koji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-65fe592461dee078efe2a6bce0752b46f687fc6f278059c01c82cbb4d24a369e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>activated protein C</topic><topic>Biological and medical sciences</topic><topic>Blood coagulation. Blood cells</topic><topic>Blood Platelets - drug effects</topic><topic>Blood Platelets - metabolism</topic><topic>Coumarins</topic><topic>Factor Xa - metabolism</topic><topic>Factor Xa - pharmacology</topic><topic>Fluorescent Dyes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Molecular and cellular biology</topic><topic>Oligopeptides</topic><topic>Platelet</topic><topic>platelet aggregation</topic><topic>Platelet Aggregation - drug effects</topic><topic>Platelet Aggregation - physiology</topic><topic>Platelet Aggregation Inhibitors - pharmacology</topic><topic>Protein C - metabolism</topic><topic>Protein C - pharmacology</topic><topic>Prothrombin - metabolism</topic><topic>Prothrombin - pharmacology</topic><topic>thrombin</topic><topic>Thrombin - biosynthesis</topic><topic>Thrombin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Komoriya, Keiji</creatorcontrib><creatorcontrib>Inoue, Kyoko</creatorcontrib><creatorcontrib>Takahashi, Katsushi</creatorcontrib><creatorcontrib>Suzuki, Koji</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Thrombosis research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Komoriya, Keiji</au><au>Inoue, Kyoko</au><au>Takahashi, Katsushi</au><au>Suzuki, Koji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effect of activated protein C on platelet aggregation induced by the prothrombin-converting reaction</atitle><jtitle>Thrombosis research</jtitle><addtitle>Thromb Res</addtitle><date>1992-09-15</date><risdate>1992</risdate><volume>67</volume><issue>6</issue><spage>705</spage><epage>710</epage><pages>705-710</pages><issn>0049-3848</issn><eissn>1879-2472</eissn><coden>THBRAA</coden><abstract>The present study was undertaken to elucidate the effect on platelet aggregation of the prothrombin-converting reaction on platelets with or without activated protein C (APC). A reaction mixture of washed platelets from human individuals, Factor Xa and prothrombin markedly induced platelet aggregation; maximum aggregation rates, 31.3–92.5%, and times to reach to maximum aggregation, 11.6 to 20.1 min. This aggregation was inhibited by the addition of APC with 50% inhibition concentration (IC
50) value of 14.4 U/ml. APC also inhibited thrombin generation in the reaction mixture in a dose-dependent manner with IC50 value of 0.96 U/ml. However, APC did not inhibit the thrombin (0.1 CU/ml)-induced platelet aggregation at concentrations of up to 30 U/ml. These findings suggest that APC has no direct inhibitory effect on platelet aggregation and that APC inhibits platelet aggregation through inhibition of thrombin generation.</abstract><cop>New York, NY</cop><pub>Elsevier Ltd</pub><pmid>1440535</pmid><doi>10.1016/0049-3848(92)90074-K</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0049-3848 |
| ispartof | Thrombosis research, 1992-09, Vol.67 (6), p.705-710 |
| issn | 0049-3848 1879-2472 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73352122 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | activated protein C Biological and medical sciences Blood coagulation. Blood cells Blood Platelets - drug effects Blood Platelets - metabolism Coumarins Factor Xa - metabolism Factor Xa - pharmacology Fluorescent Dyes Fundamental and applied biological sciences. Psychology Humans In Vitro Techniques Molecular and cellular biology Oligopeptides Platelet platelet aggregation Platelet Aggregation - drug effects Platelet Aggregation - physiology Platelet Aggregation Inhibitors - pharmacology Protein C - metabolism Protein C - pharmacology Prothrombin - metabolism Prothrombin - pharmacology thrombin Thrombin - biosynthesis Thrombin - pharmacology |
| title | Inhibitory effect of activated protein C on platelet aggregation induced by the prothrombin-converting reaction |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T04%3A44%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inhibitory%20effect%20of%20activated%20protein%20C%20on%20platelet%20aggregation%20induced%20by%20the%20prothrombin-converting%20reaction&rft.jtitle=Thrombosis%20research&rft.au=Komoriya,%20Keiji&rft.date=1992-09-15&rft.volume=67&rft.issue=6&rft.spage=705&rft.epage=710&rft.pages=705-710&rft.issn=0049-3848&rft.eissn=1879-2472&rft.coden=THBRAA&rft_id=info:doi/10.1016/0049-3848(92)90074-K&rft_dat=%3Cproquest_cross%3E73352122%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73352122&rft_id=info:pmid/1440535&rft_els_id=004938489290074K&rfr_iscdi=true |