Reverse hybridization and sequencing for genotyping the hepatitis C virus
The methods for genotyping the hepatitis C virus have been much discussed. The aim of this study was to compare the methodologies of reverse hybridization and direct sequencing for genotyping the hepatitis C virus. Ninety-one plasma samples from patients attended at the Botucatu Medical School, São...
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| Veröffentlicht in: | Revista da Sociedade Brasileira de Medicina Tropical 2010-03, Vol.43 (2), p.135-138 |
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| container_title | Revista da Sociedade Brasileira de Medicina Tropical |
| container_volume | 43 |
| creator | Levada, Patrícia Martinez Moraes, Camila Fernanda Verdichio de Corvino, Silvia Maria Grotto, Rejane Maria Tommasini Silva, Giovanni Faria Pardini, Maria Inês de Moura Campos |
| description | The methods for genotyping the hepatitis C virus have been much discussed. The aim of this study was to compare the methodologies of reverse hybridization and direct sequencing for genotyping the hepatitis C virus.
Ninety-one plasma samples from patients attended at the Botucatu Medical School, São Paulo State University, were used. Genotyping by reverse hybridization was performed using the INNO-LiPA(R) v.1.0 commercial kit. Direct sequencing was performed in an automated sequencer using in-house protocols.
Genotyping by direct sequencing was shown to be efficient for resolving cases that had remained inconclusive after using the commercial kit. The kit showed erroneous results in relation to virus subtyping. Moreover, direct sequencing revealed an error of the kit regarding the genotypic determination, thereby raising doubts about the efficiency of reverse hybridization for identifying the virus genotype.
Genotyping by direct sequencing allowed greater accuracy of virus classification than did reverse hybridization. |
| doi_str_mv | 10.1590/S0037-86822010000200006 |
| format | Article |
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Ninety-one plasma samples from patients attended at the Botucatu Medical School, São Paulo State University, were used. Genotyping by reverse hybridization was performed using the INNO-LiPA(R) v.1.0 commercial kit. Direct sequencing was performed in an automated sequencer using in-house protocols.
Genotyping by direct sequencing was shown to be efficient for resolving cases that had remained inconclusive after using the commercial kit. The kit showed erroneous results in relation to virus subtyping. Moreover, direct sequencing revealed an error of the kit regarding the genotypic determination, thereby raising doubts about the efficiency of reverse hybridization for identifying the virus genotype.
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Ninety-one plasma samples from patients attended at the Botucatu Medical School, São Paulo State University, were used. Genotyping by reverse hybridization was performed using the INNO-LiPA(R) v.1.0 commercial kit. Direct sequencing was performed in an automated sequencer using in-house protocols.
Genotyping by direct sequencing was shown to be efficient for resolving cases that had remained inconclusive after using the commercial kit. The kit showed erroneous results in relation to virus subtyping. Moreover, direct sequencing revealed an error of the kit regarding the genotypic determination, thereby raising doubts about the efficiency of reverse hybridization for identifying the virus genotype.
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The aim of this study was to compare the methodologies of reverse hybridization and direct sequencing for genotyping the hepatitis C virus.
Ninety-one plasma samples from patients attended at the Botucatu Medical School, São Paulo State University, were used. Genotyping by reverse hybridization was performed using the INNO-LiPA(R) v.1.0 commercial kit. Direct sequencing was performed in an automated sequencer using in-house protocols.
Genotyping by direct sequencing was shown to be efficient for resolving cases that had remained inconclusive after using the commercial kit. The kit showed erroneous results in relation to virus subtyping. Moreover, direct sequencing revealed an error of the kit regarding the genotypic determination, thereby raising doubts about the efficiency of reverse hybridization for identifying the virus genotype.
Genotyping by direct sequencing allowed greater accuracy of virus classification than did reverse hybridization.</abstract><cop>Brazil</cop><pub>Sociedade Brasileira de Medicina Tropical</pub><pmid>20464141</pmid><doi>10.1590/S0037-86822010000200006</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 5' Untranslated Regions - genetics Genome, Viral - genetics Genotype Hepacivirus - classification Hepacivirus - genetics Hepatitis Humans Hybridization Nucleic Acid Hybridization - genetics Oligonucleotide Array Sequence Analysis - methods Viral Nonstructural Proteins - genetics |
| title | Reverse hybridization and sequencing for genotyping the hepatitis C virus |
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