RAPID FETAL LUNG MATURITY TESTING: COMMERCIAL VERSUS NBD-PHOSPHATIDYLCHOLINE ASSAY
We compared the TDx Fetal Lung Maturity test and the fluorescence polarization method using l-palmitoyl-2{6-[(7- nitro-2,l,3-benzoxadiazol-4-yl)amino]caproyl}phosphatidylcholine (NBD-phosphatidylcholine). Using 76 paired human amniotic fluid samples, the fluorescence polarization values of the two m...
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| Veröffentlicht in: | Obstetrics and gynecology (New York. 1953) 1992-12, Vol.80 (6), p.1048-1053 |
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| container_title | Obstetrics and gynecology (New York. 1953) |
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| creator | Ashwood, Edward R Palmer, Stephen E Lenke, Roger R |
| description | We compared the TDx Fetal Lung Maturity test and the fluorescence polarization method using l-palmitoyl-2{6-[(7- nitro-2,l,3-benzoxadiazol-4-yl)amino]caproyl}phosphatidylcholine (NBD-phosphatidylcholine). Using 76 paired human amniotic fluid samples, the fluorescence polarization values of the two methods were found to have a strong nonlinear correlation (r2=0.946). Both assays can be completed in less than 1 hour, have excellent precision (between- day variation less than 2%), and indicate the amount of surfactant phospholipid relative to albumin. The FLM assay is calibrated with surfactant/albumin standards; therefore, the reported results (in mg/g) correlate inversely with polarization of NBD-phosphatidylcholine. Strong correlations were seen for both assays with the lecithinsphingomyelin ratio and phosphatidylglycerol. The correlations indicate that the recommended reference range for FLM will have more false predictions of immaturity than the NBD-phosphatidylcholine assay. (Obstet Gynecol 1992;801048-53) |
| format | Article |
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Using 76 paired human amniotic fluid samples, the fluorescence polarization values of the two methods were found to have a strong nonlinear correlation (r2=0.946). Both assays can be completed in less than 1 hour, have excellent precision (between- day variation less than 2%), and indicate the amount of surfactant phospholipid relative to albumin. The FLM assay is calibrated with surfactant/albumin standards; therefore, the reported results (in mg/g) correlate inversely with polarization of NBD-phosphatidylcholine. Strong correlations were seen for both assays with the lecithinsphingomyelin ratio and phosphatidylglycerol. The correlations indicate that the recommended reference range for FLM will have more false predictions of immaturity than the NBD-phosphatidylcholine assay. (Obstet Gynecol 1992;801048-53)</description><identifier>ISSN: 0029-7844</identifier><identifier>EISSN: 1873-233X</identifier><identifier>PMID: 1448250</identifier><identifier>CODEN: OBGNAS</identifier><language>eng</language><publisher>New York, NY: The American College of Obstetricians and Gynecologists</publisher><subject>Albumins - analysis ; Amniotic Fluid - chemistry ; Biological and medical sciences ; Fetal Organ Maturity ; Fluorescence Polarization - instrumentation ; Fluorescent Dyes ; Gynecology. Andrology. Obstetrics ; Lung - embryology ; Management. Prenatal diagnosis ; Medical sciences ; Phosphatidylcholines - analysis ; Phosphatidylglycerols - analysis ; Pregnancy. Fetus. Placenta ; Prenatal Diagnosis - instrumentation ; Prenatal Diagnosis - methods ; Pulmonary Surfactants - analysis ; Sphingomyelins - analysis ; Time Factors</subject><ispartof>Obstetrics and gynecology (New York. 1953), 1992-12, Vol.80 (6), p.1048-1053</ispartof><rights>1992 The American College of Obstetricians and Gynecologists</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4553968$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1448250$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ashwood, Edward R</creatorcontrib><creatorcontrib>Palmer, Stephen E</creatorcontrib><creatorcontrib>Lenke, Roger R</creatorcontrib><title>RAPID FETAL LUNG MATURITY TESTING: COMMERCIAL VERSUS NBD-PHOSPHATIDYLCHOLINE ASSAY</title><title>Obstetrics and gynecology (New York. 1953)</title><addtitle>Obstet Gynecol</addtitle><description>We compared the TDx Fetal Lung Maturity test and the fluorescence polarization method using l-palmitoyl-2{6-[(7- nitro-2,l,3-benzoxadiazol-4-yl)amino]caproyl}phosphatidylcholine (NBD-phosphatidylcholine). Using 76 paired human amniotic fluid samples, the fluorescence polarization values of the two methods were found to have a strong nonlinear correlation (r2=0.946). Both assays can be completed in less than 1 hour, have excellent precision (between- day variation less than 2%), and indicate the amount of surfactant phospholipid relative to albumin. The FLM assay is calibrated with surfactant/albumin standards; therefore, the reported results (in mg/g) correlate inversely with polarization of NBD-phosphatidylcholine. Strong correlations were seen for both assays with the lecithinsphingomyelin ratio and phosphatidylglycerol. The correlations indicate that the recommended reference range for FLM will have more false predictions of immaturity than the NBD-phosphatidylcholine assay. (Obstet Gynecol 1992;801048-53)</description><subject>Albumins - analysis</subject><subject>Amniotic Fluid - chemistry</subject><subject>Biological and medical sciences</subject><subject>Fetal Organ Maturity</subject><subject>Fluorescence Polarization - instrumentation</subject><subject>Fluorescent Dyes</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Lung - embryology</subject><subject>Management. Prenatal diagnosis</subject><subject>Medical sciences</subject><subject>Phosphatidylcholines - analysis</subject><subject>Phosphatidylglycerols - analysis</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>Prenatal Diagnosis - instrumentation</subject><subject>Prenatal Diagnosis - methods</subject><subject>Pulmonary Surfactants - analysis</subject><subject>Sphingomyelins - analysis</subject><subject>Time Factors</subject><issn>0029-7844</issn><issn>1873-233X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1Kw0AYRQdRaq0-gpCFuAvM_0zcxTQ2gTQp-RG7CtN0QquprZmG4ts70OLi4_JxD2dxr8AYSUFcTMjHNRhDiD1XSEpvwZ0xnxBCxD0yAiNEqcQMjkGe-4t46ryFpZ84SZXOnLlfVnlcLp0yLMo4nb04QTafh3kQW-I9zIuqcNLXqbuIsmIR-WU8XSZBlCVxGjp-UfjLe3DTqs7oh0tOQGX1QeQm2SwO_MQ9YAmh23KGVogiRgRhTErSCqaZENhDlGMu10Qr5XHNW6iggFRrzqBs-Io2jCJByQQ8n72Hfv8zaHOsd1vT6K5T33o_mFoQwqAgyIKPF3BY7fS6PvTbnep_68sItn-69Mo0qmt79d1szT9GGSMelxajZ-y07466N1_dcNJ9vdGqO25quy3k1uYiz8MI28-1RyD5A8jibD0</recordid><startdate>199212</startdate><enddate>199212</enddate><creator>Ashwood, Edward R</creator><creator>Palmer, Stephen E</creator><creator>Lenke, Roger R</creator><general>The American College of Obstetricians and Gynecologists</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199212</creationdate><title>RAPID FETAL LUNG MATURITY TESTING: COMMERCIAL VERSUS NBD-PHOSPHATIDYLCHOLINE ASSAY</title><author>Ashwood, Edward R ; Palmer, Stephen E ; Lenke, Roger R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2800-f651b141537355883f75e57729146268d3eaa96e6f0a0704ee6508c6b4c541743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Albumins - analysis</topic><topic>Amniotic Fluid - chemistry</topic><topic>Biological and medical sciences</topic><topic>Fetal Organ Maturity</topic><topic>Fluorescence Polarization - instrumentation</topic><topic>Fluorescent Dyes</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Lung - embryology</topic><topic>Management. Prenatal diagnosis</topic><topic>Medical sciences</topic><topic>Phosphatidylcholines - analysis</topic><topic>Phosphatidylglycerols - analysis</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>Prenatal Diagnosis - instrumentation</topic><topic>Prenatal Diagnosis - methods</topic><topic>Pulmonary Surfactants - analysis</topic><topic>Sphingomyelins - analysis</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ashwood, Edward R</creatorcontrib><creatorcontrib>Palmer, Stephen E</creatorcontrib><creatorcontrib>Lenke, Roger R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Obstetrics and gynecology (New York. 1953)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ashwood, Edward R</au><au>Palmer, Stephen E</au><au>Lenke, Roger R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RAPID FETAL LUNG MATURITY TESTING: COMMERCIAL VERSUS NBD-PHOSPHATIDYLCHOLINE ASSAY</atitle><jtitle>Obstetrics and gynecology (New York. 1953)</jtitle><addtitle>Obstet Gynecol</addtitle><date>1992-12</date><risdate>1992</risdate><volume>80</volume><issue>6</issue><spage>1048</spage><epage>1053</epage><pages>1048-1053</pages><issn>0029-7844</issn><eissn>1873-233X</eissn><coden>OBGNAS</coden><abstract>We compared the TDx Fetal Lung Maturity test and the fluorescence polarization method using l-palmitoyl-2{6-[(7- nitro-2,l,3-benzoxadiazol-4-yl)amino]caproyl}phosphatidylcholine (NBD-phosphatidylcholine). Using 76 paired human amniotic fluid samples, the fluorescence polarization values of the two methods were found to have a strong nonlinear correlation (r2=0.946). Both assays can be completed in less than 1 hour, have excellent precision (between- day variation less than 2%), and indicate the amount of surfactant phospholipid relative to albumin. The FLM assay is calibrated with surfactant/albumin standards; therefore, the reported results (in mg/g) correlate inversely with polarization of NBD-phosphatidylcholine. Strong correlations were seen for both assays with the lecithinsphingomyelin ratio and phosphatidylglycerol. The correlations indicate that the recommended reference range for FLM will have more false predictions of immaturity than the NBD-phosphatidylcholine assay. (Obstet Gynecol 1992;801048-53)</abstract><cop>New York, NY</cop><pub>The American College of Obstetricians and Gynecologists</pub><pmid>1448250</pmid><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0029-7844 |
| ispartof | Obstetrics and gynecology (New York. 1953), 1992-12, Vol.80 (6), p.1048-1053 |
| issn | 0029-7844 1873-233X |
| language | eng |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Albumins - analysis Amniotic Fluid - chemistry Biological and medical sciences Fetal Organ Maturity Fluorescence Polarization - instrumentation Fluorescent Dyes Gynecology. Andrology. Obstetrics Lung - embryology Management. Prenatal diagnosis Medical sciences Phosphatidylcholines - analysis Phosphatidylglycerols - analysis Pregnancy. Fetus. Placenta Prenatal Diagnosis - instrumentation Prenatal Diagnosis - methods Pulmonary Surfactants - analysis Sphingomyelins - analysis Time Factors |
| title | RAPID FETAL LUNG MATURITY TESTING: COMMERCIAL VERSUS NBD-PHOSPHATIDYLCHOLINE ASSAY |
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